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DNA Replication
DNA Replication

DNA Sequence Analysis
DNA Sequence Analysis

... DNA sequences 1. DNA sequence databases contain genomic sequence data,which includes information at the level of the untranslated sequence, introns and exons, mRNA, cDNA , and translations. 2. Untranslated regions(UTRs): occur in both DNA and RNA; they are portions of the sequence flanking the CDS t ...
Prof. Emmanuelle Charpentier (France) Dr. Jennifer A. Doudna (USA)
Prof. Emmanuelle Charpentier (France) Dr. Jennifer A. Doudna (USA)

... sections, with one section cutting one of the strand and the other section cutting the opposite strand. The markers used for incision are short sequences approximately three-base-long called PAM (Proto-spacer Adjacent Motif) located throughout the intruder’s DNA. The Cas9 enzyme coming in contact wi ...
Pathogenic bacteria Genomic DNA extracted from
Pathogenic bacteria Genomic DNA extracted from

Biology Second Semester Study Guide Molecular Genetics (Chapter
Biology Second Semester Study Guide Molecular Genetics (Chapter

... formed some of the amino acids which are used to make proteins. Perhaps most importantly, Miller's experiment showed that organic compounds such as amino acids, which are essential to cellular life, could be made easily under the conditions that scientists believed to be present on the early earth. ...
DNA Deoxyribonucleic Acid
DNA Deoxyribonucleic Acid

... (asp) ...
Nucleotide Functions Nucleic Acid Structure Nucleic Acid Sequence
Nucleotide Functions Nucleic Acid Structure Nucleic Acid Sequence

... Ribosomal RNA • “Scaffold” for proteins involved in protein synthesis • RNA has catalytic activity as the “peptidyl transferase” which forms the peptide bond • Prokaryotes and Eukaryotes have slightly different ribosomal structures (See Figure 11.25) • Ribosomal RNA contains some modified nucleoside ...
Taxonomy
Taxonomy

... http://www.youtube.com/watch?v=76eKnmCtCTU ...
Viroids - Danziger
Viroids - Danziger

... information, which might help you, cope with this challenge. A viroid is a cell-invading plant pathogen 80 times smaller than a virus. An ARS plant pathologist Theodor O. Diener discovered it in 1971 and named it a ‘viroid’, since it acts “like a virus”. Up until then, the scientific dogma was that ...
Bio Medical Syllabus - Wilson School District
Bio Medical Syllabus - Wilson School District

... biotechnology, physical therapy, genetics, pharmacy, and related biological sciences. The course includes college level laboratory activities taught at a high school level. These hands-on activities include extensive interaction with the instructor. A high school level textbook on biotechnology is u ...
BBVCh1,2012
BBVCh1,2012

...  Requires an organism to take in material from the environment and transform it into its own structures  To accomplish this, the organism must use some of the energy it has acquired during metabolism  Instructions for growth and development: encoded in DNA VIII. Reproduction  Biogenesis, which m ...
BIOL 5870 - East Carolina University
BIOL 5870 - East Carolina University

... of DNA Organization, induction of DNA Damage and Mutagenesis, DNA Repair, Replication, Transcription, Translation, Recombination, Gene Regulation and protein expression primarily in eukaryotic organisms. At the same time a brief description will be given on several current molecular biology techniqu ...
9.4 DNA-Binding Proteins
9.4 DNA-Binding Proteins

... • R-O complex DNA deviates from normal shape • DNA bends to accommodate base /aa contacts • Central part of helix is wound extra tightly • Outer parts are wound more loosely than normal • DNA sequence of operator facilitates bending ...
Revised 2015 15.2 PowerPoint
Revised 2015 15.2 PowerPoint

... A clone is a member of a population of genetically identical cells produced from a single cell The technique of cloning uses a single cell from an adult organism to grow an entirely new individual that is genetically identical to the organism from which the cell was taken. Clones of animals were fir ...
What is a gene? - World of Teaching
What is a gene? - World of Teaching

... • But when lzs/lzg females are crossed to lzs or lzg males, about 0.2% of the progeny are wild-type! • These must result from recombination between lzs and lzg , because the wild-type progeny always had recombinant flanking markers. Also, the frequency of 0.2% is much higher than the reversion rate ...
Green, Ed, NEADERTHAL DNA, UC Santa Cruz, June
Green, Ed, NEADERTHAL DNA, UC Santa Cruz, June

... every chimp does knuckle-walking and all us walk on two feet... we were all a fertilized egg at one point one set of dna from mom and one set from dad... that is what DIPLOID means. where in that genome are these uniquely human characteristictics encoded... what makes humans unique genetically?. 35M ...
Lecture 14
Lecture 14

... PSYC 3102: Introduction to Behavioral Genetics Lecture 14 ...
By Michael Harwood This article was catalysed
By Michael Harwood This article was catalysed

... from the Newtonian stage of understanding DNA to the Einsteinian stage. We won’t know how many stages there are until we proceed to the next one and clearly look at what exactly it is that we still don’t understand. Yes, the Newtonian stage of Watson and Crick is an earth-shattering discovery, but i ...
Types of Mutations
Types of Mutations

Word file (24 KB )
Word file (24 KB )

... targeting vector, containing a promoter-less lacZ and neomycin-resistance gene under control of the RNA polymerase II promoter (gift from R. Palmiter). A 7-kb Bam HI-Xho I fragment was used as a long arm. The targeting vector was electroporated into 129 SvEv-derived SM-1 ES cells and selected under ...
Genetics = science of heredity - Suffolk County Community College
Genetics = science of heredity - Suffolk County Community College

... leading strand is synthesized continuously by the enzyme DNA polymerase in the direction of the replication fork. New tri-phosphate nucleotides from the cytoplasm/nucleoplasm are complementarily base paired with the parental strand and chemically bonded to the 3’end of the RNA primer and subsequentl ...
Heredity
Heredity

... What are the potential dangers of biotechnology? (A) Vocabulary: translation, transcription, mRNA, tRNA, anticodon, amino acid, protein, rRNA, codon, protein synthesis, intron, exon, RNA polymerase, gene expression, gene mutation, chromosomal mutation, inversion, point mutation, frameshift mutation, ...
Biology DNA and Protein Syn
Biology DNA and Protein Syn

... • How do genes work? How do they determine the characteristics of organisms? • To truly understand genetics, biologists first had to learn the chemical composition of a gene. • It took the work of many scientists over several years to identify DNA as the genetic material, and to discover its structu ...
From Bugs to Barcodes: Using Molecular Tools to Study
From Bugs to Barcodes: Using Molecular Tools to Study

... • Thus universal primers are not species specific • This means that the primers are probably not totally complementary to the sequence in your insect sample but they are similar enough to hybridize at a low annealing temperature sample – The sequence in the CO1 gene in between where the primers hybr ...
What is a gene?
What is a gene?

... • But when lzs/lzg females are crossed to lzs or lzg males, about 0.2% of the progeny are wild-type! • These must result from recombination between lzs and lzg , because the wild-type progeny always had recombinant flanking markers. Also, the frequency of 0.2% is much higher than the reversion rate ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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