• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Last Name: First Name: Per. _____ Parent Signature: Pre
Last Name: First Name: Per. _____ Parent Signature: Pre

... A mutation is a random change in DNA code that occurs during DNA replication or through a failure in repair mechanisms. The significance is that mutation is the primary source of genetic diversity – it is how we get brand new alleles. Genetic diversity is necessary for evolution through natural sele ...
I-Modified Nucleosides as DNA-Sugar Centered Radical Precursors
I-Modified Nucleosides as DNA-Sugar Centered Radical Precursors

... C1’ radicals The C1’-centered radical 1.1 can be generated by hydrogen abstraction through the interaction of DNA, oligonucleotides or nucleosides with ionizing radiation (γ-radiolysis), metal complexes (Mn-TMPyP, Cu(oP)2 and anticancer drugs such as enediynes). Photolysis of photoreactive groups th ...
The fate of transgenes in the human gut
The fate of transgenes in the human gut

Transgenic plant Herbicide Resistance
Transgenic plant Herbicide Resistance

Biological Diversity Section 3 Student Notes
Biological Diversity Section 3 Student Notes

... Organisms have two copies of each gene which may be the same or different. Different versions of a gene are called alleles ...
Mortlock_lab_Nucleobond_maxiprep
Mortlock_lab_Nucleobond_maxiprep

... DNA. The 260/280 absorbance ratio should be approx. 1.85, and the 260/230 absorbance ratio should be greater than 2.0. It is also critical to check that the DNA really is BAC DNA by agarose gel analysis of a restriction digest. ...
Supplemental Data
Supplemental Data

SBI3U Genetics Review
SBI3U Genetics Review

... -be able to briefly describe the technology and its use / purpose -cloning (p 119) -in-vitro fertilization, surrogate mothers (p134-5) -amniocentesis, ultrasound, chorionic villi sampling, X-rays (p131) ...
DNA STRUCTURE - Teachers Network
DNA STRUCTURE - Teachers Network

... Sugar (What does the D stand for? What does the R Bases used (A,T,G,C,U) 8. How do you use the amino acid table? The RNA sequence AUG codes for the amino acid ________________________________. The RNA sequence UGC codes for the amino acid ________________________________. The RNA sequence AAG codes ...
03/24
03/24

... Minimal medium contains only inorganic salts, simple carbon source, and water. Auxotrophs require nutritional supplement(s) for growth. ...
Chapter 5A
Chapter 5A

... (ts) phenotypes are used. ts mutations often result from substitution mutations that cause an essential protein to be unstable and inactive at high (nonpermissive), but not low (permissive) temperatures. A number of yeast cell-division cycle (cdc) mutants have been isolated via this technique (Fig. ...
Protein Synthesis SG
Protein Synthesis SG

... 18. Draw and label the parts of charged tRNAs given a DNA sequence. 19. Describe the different base substitution mutations and the results of each. 20. What is a frameshift mutation and how does it affect the outcome of the protein? 21. Is the result of a point or frameshift mutation more harmful to ...
Protein/Nucleic acid
Protein/Nucleic acid

... Dehydration synthesis -> covalent bond aka phosphodiester between hydroxyl & phosphate groups polymer ‘backbone’ ...
second of four for Chapter 9
second of four for Chapter 9

... DNA fragment gets incorporated into the recipient chromosome. • This will result in the F- cell becoming recombinant, but the Hfr cell stays the same. ...
ADP: adenine diphosphate. The low-energy form of ATP. Contains
ADP: adenine diphosphate. The low-energy form of ATP. Contains

... hydrogen atom) are transferred from one molecule (which is thus oxidized) to another molecule (which is thus reduced). Ribosomal RNA: The component of the Ribosome made of RNA Ribosome: a complex found in cells, made up of several subunits, each composed of proteins and RNA. Ribosomes are the site o ...
DNA FINGERPRINTING
DNA FINGERPRINTING

Lab 8
Lab 8

... In our cells, DNA is found inside the nucleus, wrapped around basic protein molecules called histones (kind of like thread wrapped around a spool). This combination of DNA and protein is called a nucleosome. The DNA does not leave the nucleus, so when new proteins or other structures need to be made ...
dna - Kowenscience.com
dna - Kowenscience.com

... They proposed that when the time came for DNA to be replicated, the two strands of the molecule: 1. separated from each other but 2. remained intact as each served as the template for the synthesis of 3. a complementary strand. • When the replication process is complete, two DNA molecules — identica ...
IB104 - Lecture 15
IB104 - Lecture 15

... methyl group (-CH3), is attached to the single-ring base of the cytosine when it occurs as a CpG (not a base pair, but a sequential pair of nucleotides along a strand – p means phosphate). This “mark” is present on the DNA of many different kinds of organisms, and has several diverse roles, but the ...
LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

... 11. A property of normal cell to give rise to a complete individual is called ------------. 12. A fusion product with cytoplasm of both the cells involved and nucleus of only one cell is called ---------. 13. Ri- plasmid causes --------------------- disease in plants. 14. A process by which proteins ...
Recombinant DNA and Genetic Engineering
Recombinant DNA and Genetic Engineering

... 2 Virus enters host cell. host cell 3 Virus releases its DNA into host cell; some viral DNA (red) may be incorporated into the host cell’s DNA (blue). ...
DNA - Midlakes
DNA - Midlakes

... Under each DNA sequence in your answer packet, write the complimentary messenger RNA base sequence. Note: Unlike during DNA replication, in the production of messenger RNA, the DNA base “A” specifies the RNA base “U”. Write the complimentary sequence in your answer packet. ...
DNA to Protein - Seabreeze High School
DNA to Protein - Seabreeze High School

... Things to think About & Discuss 1. What if a mutation occurs in the DNA? Explain how could that affect the organism’s protein? 2. What if a mutation occurs in 3rd base of the codon? Will it always code for a different amino acid? Explain. ...
DNA upgrade supplement WITH PICS
DNA upgrade supplement WITH PICS

... purines are adenine and guanine. These bases are commonly abbreviated as C, T, A, and G. Also, it helps to remember which nitrogenous bases are pyrimidines if you note that thymine and cytosine are spelled with a "Y," and so is pyrimidine. Scene 13: Levene also discovered one phosphate group and one ...
MCAS Biology Review
MCAS Biology Review

... organism with a dominant allele for a particular form of a trait will always exhibit that form of the trait. An organism with a recessive allele for a particular form of a trait will exhibit that form only ...
< 1 ... 492 493 494 495 496 497 498 499 500 ... 766 >

Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
  • studyres.com © 2026
  • DMCA
  • Privacy
  • Terms
  • Report