DNA Fingerprinting: The Code to Identification
... bones found in a shared grave in Russia. The victims turned out to be members of the royal family, the Romanovs, who had been executed in 1918. Because the Y chromosome, part of the nuclear genome, is passed largely intact from father to son for many generations, DNA fingerprinting of the Y chromoso ...
... bones found in a shared grave in Russia. The victims turned out to be members of the royal family, the Romanovs, who had been executed in 1918. Because the Y chromosome, part of the nuclear genome, is passed largely intact from father to son for many generations, DNA fingerprinting of the Y chromoso ...
Homework Assignment #1
... Answer: SL-1, TFIIIB, and TFIID all contain the TATA binding protein (TBP). Each of these transcription factors determine where the start site of transcription will be and thus must help either directly or indirectly position RNA polymerase over the start site. SL-1 and TFIIIB are both known to dir ...
... Answer: SL-1, TFIIIB, and TFIID all contain the TATA binding protein (TBP). Each of these transcription factors determine where the start site of transcription will be and thus must help either directly or indirectly position RNA polymerase over the start site. SL-1 and TFIIIB are both known to dir ...
What happened? Conjugation requires Plasmids
... Conjugation requires Plasmids • Conversion was not efficient (about 1 in 10,000,000 cells) • Physical contact between the two auxotrophic cell types was necessary • Simply allowing free flow of the medium, but keeping the cells apart, did not produce prototrophs; so this was not a kind of transforma ...
... Conjugation requires Plasmids • Conversion was not efficient (about 1 in 10,000,000 cells) • Physical contact between the two auxotrophic cell types was necessary • Simply allowing free flow of the medium, but keeping the cells apart, did not produce prototrophs; so this was not a kind of transforma ...
TOPIC 4.4 Genetic Engineering Worksheet
... This link is to the Wellcome Institute Sanger Centre’s Genome website:http://www.yourgenome.org This link is to the US Government National Human Genome Website: http://www.genome.gov 1. What is the the Human Genome project? When did it begin, and when was the ‘first draft’ published? ...
... This link is to the Wellcome Institute Sanger Centre’s Genome website:http://www.yourgenome.org This link is to the US Government National Human Genome Website: http://www.genome.gov 1. What is the the Human Genome project? When did it begin, and when was the ‘first draft’ published? ...
19. IMG-ER Curation Environment
... EC number and PUBMED ID – see explanation Notes are free text (goes to “note” in GenBank submission) Gene symbol is “gene name” – 4 letter abbreviation; goes to “gene” in GenBank submission ...
... EC number and PUBMED ID – see explanation Notes are free text (goes to “note” in GenBank submission) Gene symbol is “gene name” – 4 letter abbreviation; goes to “gene” in GenBank submission ...
Bio 104 Exam 2 Review
... Viruses – Considered non-living (why?) Can infect any cell type – those that infect bacteria are called bacteriophage Outer capsid – composed of protein Inner core – composed of DNA or RNA Two replication cycles – Lytic and Lysogenic (know differences), prophage Prion diseases – infectious protein ( ...
... Viruses – Considered non-living (why?) Can infect any cell type – those that infect bacteria are called bacteriophage Outer capsid – composed of protein Inner core – composed of DNA or RNA Two replication cycles – Lytic and Lysogenic (know differences), prophage Prion diseases – infectious protein ( ...
Genome Editing Slides
... • Discovered as what prokaryotes have as an immune system • Pallindromic Repeats of 20-40 bases, separated by short sequences that turn out to be leftover from bacterial viruses that had previously infected the cell – Pallindromic DNA, when transcribed make RNA’s that can base pair with themselves t ...
... • Discovered as what prokaryotes have as an immune system • Pallindromic Repeats of 20-40 bases, separated by short sequences that turn out to be leftover from bacterial viruses that had previously infected the cell – Pallindromic DNA, when transcribed make RNA’s that can base pair with themselves t ...
The Cell Cycle - Issaquah Connect
... These cells are often: dying being worn away or the organism may be growing and needs more cells… ...
... These cells are often: dying being worn away or the organism may be growing and needs more cells… ...
Slide 1
... • Nucleotides are bound together to form nucleic acids. – The sequence of bases is like an instruction manual that can be read by the cell. ...
... • Nucleotides are bound together to form nucleic acids. – The sequence of bases is like an instruction manual that can be read by the cell. ...
In the „restriction endonucleases”
... to be competent. Experiments have shown that if the cells are exposed to high concentrations of bivalent cations (e.g. Ca2+), the cell membranes become leaky. The DNA to transform (or a few µl from the ligation reaction) has to be added to the cells kept on ice, a brief heat shock (42°C) is needed a ...
... to be competent. Experiments have shown that if the cells are exposed to high concentrations of bivalent cations (e.g. Ca2+), the cell membranes become leaky. The DNA to transform (or a few µl from the ligation reaction) has to be added to the cells kept on ice, a brief heat shock (42°C) is needed a ...
Biology 3 Questions 1. Which is found in prokaryotic cell? (Cell)
... 70. Consider a DNA undergoes 4 rounds of replication. What percent of the double strand DNA produced contains part of the original DNA? (DNA/RNA) 71. Which is false about enzyme? (Enzyme) a) A competitive inhibitor resembles the substrate and competes for the active site b) The active site may conta ...
... 70. Consider a DNA undergoes 4 rounds of replication. What percent of the double strand DNA produced contains part of the original DNA? (DNA/RNA) 71. Which is false about enzyme? (Enzyme) a) A competitive inhibitor resembles the substrate and competes for the active site b) The active site may conta ...
problem set
... a repression domain. Some TFs also contain a ligand binding domain that regulates activity. Domains typically are joined together in a single polypeptide by flexible linker sequences that serve as hinges and allow conformational changes needed for activation/repression. Some examples of transcriptio ...
... a repression domain. Some TFs also contain a ligand binding domain that regulates activity. Domains typically are joined together in a single polypeptide by flexible linker sequences that serve as hinges and allow conformational changes needed for activation/repression. Some examples of transcriptio ...
Slide 1
... The amino acids join together to form a protein molecule. Each gene contains the sequence of bases for one protein. Why is the sequence of bases in DNA called the genetic code? © Boardworks Ltd2004 ...
... The amino acids join together to form a protein molecule. Each gene contains the sequence of bases for one protein. Why is the sequence of bases in DNA called the genetic code? © Boardworks Ltd2004 ...
Lecture 2: Overview of biochemistry
... nucleus to the ribosomes Ribosomal RNA (rRNA): Key parts (including all the catalytic functions) of ribosomes Transfer RNA (tRNA): Recognize complementary sequences on mRNA and carry amino acids for the synthesis of proteins in the ribosome Regulation: Some RNAs, including some very small ones, have ...
... nucleus to the ribosomes Ribosomal RNA (rRNA): Key parts (including all the catalytic functions) of ribosomes Transfer RNA (tRNA): Recognize complementary sequences on mRNA and carry amino acids for the synthesis of proteins in the ribosome Regulation: Some RNAs, including some very small ones, have ...
Biotechnology - MRS PITOC
... 1.1. Give at least two examples of agricultural crops and at least two examples of farm animals that have been genetically modified and the improved characteristics that have been imported on them. 1.2. Cite the major points for and against genetically modified organisms ((GMOs). 2. Recognize potent ...
... 1.1. Give at least two examples of agricultural crops and at least two examples of farm animals that have been genetically modified and the improved characteristics that have been imported on them. 1.2. Cite the major points for and against genetically modified organisms ((GMOs). 2. Recognize potent ...
Genomics Glossary - College of American Pathologists
... would not otherwise be found in biological organisms. Recombinant DNA is possible because DNA molecules from all organisms share the same chemical structure; they differ only in the sequence of nucleotides within that identical overall structure. Consequently, when DNA from a foreign source is linke ...
... would not otherwise be found in biological organisms. Recombinant DNA is possible because DNA molecules from all organisms share the same chemical structure; they differ only in the sequence of nucleotides within that identical overall structure. Consequently, when DNA from a foreign source is linke ...
The Cell Cycle
... Living things are often made of TRILLIONS of cells These cells are often: dying being worn away or the organism may be growing and needs more cells… ...
... Living things are often made of TRILLIONS of cells These cells are often: dying being worn away or the organism may be growing and needs more cells… ...
Protocol for inserting transgene using Tn7 (6/1/06) This system uses
... Protocol for inserting transgene using Tn7 (6/1/06) This system uses Tn7 to insert transgenes at a defined neutral site in the chromosome (attTn7). The site is highly conserved and is known to work as a Tn7 attachment site in E. coli and its relatives. The attTn7 sequence is conserved in most (all) ...
... Protocol for inserting transgene using Tn7 (6/1/06) This system uses Tn7 to insert transgenes at a defined neutral site in the chromosome (attTn7). The site is highly conserved and is known to work as a Tn7 attachment site in E. coli and its relatives. The attTn7 sequence is conserved in most (all) ...
Slide 1 - Indiana University–Purdue University Indianapolis
... The material of inheritance within the nucleus of a cell is arranged in long strands called chromosomes. On the molecular level the chromosomes are actually nothing more than two long strings of DNA wound together in a spiral-like structure called a double helix. ...
... The material of inheritance within the nucleus of a cell is arranged in long strands called chromosomes. On the molecular level the chromosomes are actually nothing more than two long strings of DNA wound together in a spiral-like structure called a double helix. ...
Final Review
... ○ i. Replication is a semiconservative process; one strand = the template for new, complementary strand. ○ ii. Replication requires DNA polymerase plus many other enzymes, occurs bidirectionally, and differs in the production of the leading and lagging strands. ● Genetic information in retroviruses ...
... ○ i. Replication is a semiconservative process; one strand = the template for new, complementary strand. ○ ii. Replication requires DNA polymerase plus many other enzymes, occurs bidirectionally, and differs in the production of the leading and lagging strands. ● Genetic information in retroviruses ...
Chapter 15
... protein synthesis to occur. This is accomplished by activating enzymes called aminoacyl-tRNA synthetases. There is one enzyme for each of the 20 amino acids. • tRNA molecules are bifunctional; they need to interact with amino acids and mRNA molecules. • There is an acceptor stem, where amino acid bi ...
... protein synthesis to occur. This is accomplished by activating enzymes called aminoacyl-tRNA synthetases. There is one enzyme for each of the 20 amino acids. • tRNA molecules are bifunctional; they need to interact with amino acids and mRNA molecules. • There is an acceptor stem, where amino acid bi ...
Key Concepts Select the term that best completes the
... 4 pointsfor a response that correctly describes the chromosomes at the end of each stage Sample: After meiosis I, each of two daughter cells has one set of doubled homologs. After meiosis II is complete, there are four daughter cells, each of which has four chromosomes-one set of homologs. 3 points: ...
... 4 pointsfor a response that correctly describes the chromosomes at the end of each stage Sample: After meiosis I, each of two daughter cells has one set of doubled homologs. After meiosis II is complete, there are four daughter cells, each of which has four chromosomes-one set of homologs. 3 points: ...
Cre-Lox recombination
In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.