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... The same technology, which is so helpful in one application, can be a cause of concern when applied in a different way. In 1996, scientists produced the first clone of a mammal, a sheep named Dolly. All of Dolly’s DNA came from a single body cell of another sheep. The ability to clone such a complex ...
Genetic Engineering
Genetic Engineering

... • Reproductive cloning: making animals that are genetically identical one organism with useful ...
Techniques
Techniques

... _________________________ used for RNA and DNA separation ________________________ gel electrophoresis is used for protein separation ...
16Discovery Of DNA
16Discovery Of DNA

... • Hershey and Chase found that when the bacteria had been infected with T2 phages that contained radiolabeled proteins, most of the radioactivity was in the supernatant (shell), not in the pellet (core material. • When they examined the bacterial cultures with T2 phage that had radio-labeled DNA, m ...
Ch. 12 Quiz! Get Out A Piece of Paper!
Ch. 12 Quiz! Get Out A Piece of Paper!

... DNA backbone b) hydrogen bonds hold base pairs together and the DNA backbone c) covalent bonds hold base pairs together and hydrogen bonds hold the backbone together d) hydrogen bonds hold the base pairs together and covalent bonds hold the backbone together ...
16.7 Screening for clinically important genes
16.7 Screening for clinically important genes

... • If complementary fragments are not present, the DNA probe will not be taken up and the X-ray film will not be unexposed. ...
View Slide Presentation - Association for Pathology Informatics
View Slide Presentation - Association for Pathology Informatics

... Example: cancer cell line database “stripped” down version of SIGMA  database of pre-processed data  Poster #104  Case #1: Examining a single sample for copy number aberrations  Case #2: Identifying recurrent alterations in lung adenocarcinoma ...
Document
Document

... genes to turn them on or off. Stay tuned. ...
PART III. PROTEIN SYNTHESIS SATISFIES: How DNA Makes It A
PART III. PROTEIN SYNTHESIS SATISFIES: How DNA Makes It A

... along with all the blue mRNA (messenger-RNA) nucleotides scattered next to it. This represents the contents of the nucleus. 4. Now, on the left side of the membrane (in the "cytoplasm"), place the "ribosome" surface in a horizontal position across the bottom of that area, and scatter the yellow tRNA ...
article in press
article in press

... difficult to fit into classical models, led us to consider the hypothesis that the genome might be organized in loops maintained at their bases by DNA crossings (Fig. 1). The first characteristic of this model is that non-protein-coding sequences are not considered as functionless, but instead play a f ...
Discovery of DNA
Discovery of DNA

... ● Mice injected with... ○ heat-killed smooth bacteria lived ○ heat-killed smooth and living rough bacteria died ...
Slide 1
Slide 1

... rearranged VDJ regions and the Cµ gene and another switch site before one of the other heavy chain constant region genes. • This recombination event brings the VDJ region close to one of the other constant region genes and allows expression of a new class of heavy chain. ...
Genetic Engineering
Genetic Engineering

... How do we do mix genes? • Genetic engineering – find gene – cut DNA in both organisms – paste gene from one creature into other creature’s DNA – insert new chromosome into organism – organism copies new gene as if it were its own – organism reads gene as if it were its own – organism produces NEW p ...
07 NucleicAcids-06b
07 NucleicAcids-06b

...  Nucleoside -- portion of a nucleotide without the phosphate group ...
powerpoint show
powerpoint show

... eg. Grant’s disease is a genetic disease that affects breathing The Grant’s disease mutation is on Chromosome 7 A mutation in a potassium pump is linked to Grant’s disease (The two above will be the main sections) Expression of wild type potassium pump reverts Grant’s disease effects in cultured cel ...
Slide 1
Slide 1

... A. encode transcription factors that control the expression of genes responsible for specific anatomical structures. B. are found only in Drosophila and other arthropods. C. encode proteins that form anatomical structures in the fly. D. are responsible for patterning during plant development. ...
sg 13
sg 13

... to submit these for grading – I am expecting you to work through these problems. If you understand and can complete these questions, you are well on your way to understand the material from this chapter. ...
DNA/RNA.lecture
DNA/RNA.lecture

... b. peptide bond formation c. translocation d. repeat 3. chain termination stop codon release factor E. polyribosomes/polysomes F. proteins are folding into their final shape G. post-translational modifications 1. modified 2. signal sequences of protein a. proteins for the RER 1) signal peptide/seque ...
15-Work-Experience - College Admissions Strategies
15-Work-Experience - College Admissions Strategies

The sequence of amino acids
The sequence of amino acids

Ch 12 Molecular Genetics
Ch 12 Molecular Genetics

... following base pairing rules, bond on the leading strand of DNA  Like DNA replication controlled by many enzymes Occurs in the nucleus ...
Exam III 1710 F '01 Sample.doc
Exam III 1710 F '01 Sample.doc

... A human autosomal recessive lethal genetic disease whose defective allele has been maintained at a relatively high level in certain population groups because it gives the heterozygote resistance to an infectious disease is: a. ...
BIOL1003 Sample
BIOL1003 Sample

... o The   phosphate   group   (attached   to   the   5'-­‐C   of   the   sugar)   joins   with   the   hydroxyl  (OH)  group  attached  to  the  3'-­‐C  of  the  sugar.   o This   results   in   a   phosphodiester  bond   (type   of   coval ...
The GC-content is very variable in different geneome regions
The GC-content is very variable in different geneome regions

... Amino acids coding table evolved to minimize mutations therefore this codons organization suggests C↔T and A↔G mutations to be more frequent. In his storic paper “Codon—anticodon pairing: The wobble hypothesis” [16], Crick suggests that, due to the degeneracy of the genetic code, wobble base pairs a ...
DNA Fingerprinting: The Code to Identification
DNA Fingerprinting: The Code to Identification

... bones found in a shared grave in Russia. The victims turned out to be members of the royal family, the Romanovs, who had been executed in 1918. Because the Y chromosome, part of the nuclear genome, is passed largely intact from father to son for many generations, DNA fingerprinting of the Y chromoso ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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