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2368AOS1-genefunctiongenesinaction2
2368AOS1-genefunctiongenesinaction2

...  Some genes are only active during the embryonic period whilst others such as Huntington’s disease are only expressed in the phenotype only when the individual is well into adulthood.  Some genes are only active in certain tissues (eg. Genes that produce insulin are only active in the pancreas).  ...
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MBP 1022, LECTURE 1 – Oct 27, 2000

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... “To do” list and tips for studying: Form a small study group and discuss all of the following.  Understand all terms above and be able to put them into context. Pick three words (at random) and be able to form one sentence, incorporating all ideas together.  Review all journal entries, notes, and ...
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... but it will be helpful for the future). Also you can output on the screen any comments and remarks about the task, like some observations that you made. For example: "Only two out of the three polypeptide sequences would provide a complete protein for the third one contains stop codons within its se ...
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... From DNA to proteins – information flow • when the cell divides DNA is replicated – DNA polymerase • - DNA contains genes that code for proteins • - when activated, RNA polymerase builds an RNA copy, messenger RNA (mRNA) – transcription • - mRNA goes to the ribosome – there transfer RNA (tRNA) help ...
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... For twenty years now it has been possible to get DNA to evolve in a test tube, Dr. Benner explained. A process known as the "polymerase chain reaction" (of PCR) allows DNA to make copies of itself, and then to have the copies copied, and then to have the copies of the copies copied, and so on. The c ...
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... There was a time when to amplify DNA, You had to grow tons and tons of tiny cells. Then along came a guy named Dr. Kary Mullis, Said you can amplify in vitro just as well. Just mix your template with a buffer and some primers, Nucleotides and polymerases, too. Denaturing, annealing, and extending. W ...
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... Transcription(in the nucleus) RNA polymerase unzips DNA and copies it into RNA. A’s connect with U’s and G’s connect with C’s. The starting point of transcription is known as the Promoter, the end is known as the terminal signal. After transcription the mRNA moves into the cytosol for protein synth ...
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Nucleic Acids and Protein Synthesis - Liceo da Vinci

... molecules containing anywhere from several hundred to several thousand ribonucleotides, depending on the size of the protein to be made. Each of the 100,000 or so proteins in the human body is synthesized from a different mRNA that has been transcribed from a specific gene on DNA. "Why do we need mR ...
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... irradiated DNA samples were analysed with HPLC, LC MS/MS and XPS to compare the yield of DNA damage and find out new types of DNA damage. 3. Results and discussion We confirm that LEEs can indirectly generate DNA damage through DEA resonant process and HEE can directly generate DNA damage through on ...
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... The process of DNA replication is termed semi-conservative, as the two strands of the original DNA molecule separate and each gives rise to a new complementary strand. This mechanism ensures that the genetic material is copied exactly. DNA replication begins when a region of double-stranded DNA unwi ...
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... U3 region is the binding site for a number of cellular transcription factors A TATA box is present upstream (U3/R segments) allowing transcription initiation to begin by RNA Pol II Transcription begins at the junction of U3/R and proceeds through the whole genome A Poly(A) signal directs cleavage of ...
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7. One gene one protein

... 20 amino acids = 20 triplets How many bases code for a protein of 20 amino acids? ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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