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cells
cells

... 62. How many alleles of each gene are passed on from each parent to make a child’s genotype? -Half from each parent -ex hair color, eye color ...
Chap 8 Recombinant DNA technology Fall 2012
Chap 8 Recombinant DNA technology Fall 2012

... – Medical diagnosis – Patient specimens can be examined for presence of gene sequences unique to certain pathogens ...
Slide 1
Slide 1

... 20. In mice gray fur (G) is always dominant over creamcolored fur (g). Create a Punnet square to show the cross between a homozygous gray mouse and a cream-colored mouse. Illustrate on promethean board. 21. What percentage of the mice in the F1 generation would have gray fur? ...
Terminator
Terminator

... Types of Mutations • Point mutations (substitutions) – change in a single nucleotide – Due to redundancy of the genetic code it may change the amino acid, it may not “wobble” – Silent mutations do not change the protein Normal hemoglobin DNA ...
PROTEIN SYNTHESIS What is a gene?
PROTEIN SYNTHESIS What is a gene?

... Types of Mutations • Point mutations (substitutions) – change in a single nucleotide – Due to redundancy of the genetic code it may change the amino acid, it may not “wobble” – Silent mutations do not change the protein Normal hemoglobin DNA ...
(Submitted) Genetic Synthesis of Periodic Protein Materials M. J.
(Submitted) Genetic Synthesis of Periodic Protein Materials M. J.

... comprising odd numbers of amino acids) do not allow adjoining @-strand stems to align in complete register. The results with repetitive peptide 4 indicate that this requirement can be satisfied by at least some two-amino acid turns. 5. CONCLUSIONS We consider that the biological feasibility of our g ...
Advanced Higher Cells and Proteins
Advanced Higher Cells and Proteins

... • Proteins including enzymes are three-dimensional and have a specific shape or conformation. • As a ligand binds to a protein binding site, or a substrate binds to an enzyme’s active site, the conformation of the protein changes. • This change in conformation causes a functional change in the prote ...
Nucleotides and Nuclic Acids
Nucleotides and Nuclic Acids

... •Most DNA in the cell is in the B form. •A small amount of the DNA in the cell may locally adopt the Z conformation (where sequence has alternating purine and pyrimidine bases). •The A form of DNA is found in dehydrated samples of DNA but not normally in the cell. However, double-stranded RNA and DN ...
Microbial Genetics - University of Montana
Microbial Genetics - University of Montana

... • Lederberg & Tatum, 1947 ...
Human Genetic Mutations
Human Genetic Mutations

... (add this to the bottom of your page) ...
Genetic engineering
Genetic engineering

... allowed them to eat almost anything and convert it into the food, milk, clothing, and fertilizer that humans need, also made them essential to the rise of civilization. As cattle became items of production, they were genetically bred to yield the maximum amount of meat and milk. The recent use of as ...
Nutrigenomics – taking Nutritional Medicine to the next
Nutrigenomics – taking Nutritional Medicine to the next

... unless it is bound to biopolymers of gliadin. The tiny amounts of gliadin are essential for two reasons: 1) The gliadin protects the SOD against degradation of the enzyme in the gut and 2) the gliadin is responsible for the nutrigenomic activation of the genes which code for the cell’s three Antioxi ...
DNA and the Genome - Speyside High School
DNA and the Genome - Speyside High School

... Multi-cellular organisms are comprised of a large number of cells. These are specialised to carry out specific roles in the body. Differentiation is the process by which unspecialised cells become altered and adapted to form a special function in the body. CFE Higher Biology ...
DNA: the indispensable forensic science tool
DNA: the indispensable forensic science tool

... • many randomly repeated sequences (VNTR’s and STR’s) are located within genes or coding , functional DNA sites(loci) • number of randomly repeated sequences varies between individuals at each site ...
DNA Barcoding
DNA Barcoding

... for naming and classifying organisms in 1735. We still use this system today, and call it taxonomy. In Linnaean taxonomy, all the different kinds of living organisms can be organized practically into groupings with shared characteristics, where every species can be given a unique name. Biologists of ...
Biology DA Review
Biology DA Review

... combat disease ...
Transcription and Translation Candy Activity
Transcription and Translation Candy Activity

... of the 4 RNA nucleotides clearly labeling: ribose, base, phosphate group and nucleotide name. ...
Electrophoresis
Electrophoresis

... Double Stranded DNA is denatured by heat into single strands. Short Primers for DNA replication are added to the mixture. ...
How do we find a knockout for AT4G37790 and what is this
How do we find a knockout for AT4G37790 and what is this

... primers, then t-DNA primer+RV primer because orientation is complementary. Plants homozygous for the mutant allele prove that knocking out AT4G37790 is not embryo lethal. We can phenotype these plants to see how they are lacking in development. ...
AQA Biology: Genetics, populations, evolution
AQA Biology: Genetics, populations, evolution

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AQA Biology: Genetics, populations, evolution
AQA Biology: Genetics, populations, evolution

... Maximum 3 marks ...
Genome Shock in Polyploid Plants
Genome Shock in Polyploid Plants

Chapter 8 - Laboratory Animal Boards Study Group
Chapter 8 - Laboratory Animal Boards Study Group

... 35. VNDR – variable number of dinucleotide repeats 36. Restriction fragment length polymorphisms; produced when DNA is digested with restriction enzymes, which cut DNA at specific sites; they are used as markers to detect polymorphisms in the sequence of single-copy DNA 37. e 38. b, d, e, c, a, f 39 ...
Competence
Competence

... up and incorporated into the cellular DNA? As shown in Fig. 6.8, transforms were observed depending on the time the DNA was extracted from the cells. 1. Time 1, the DNA is still outside the cells and accessible to the DNase. No Arg+ transformants are observed because the Arg+ donor DNA is all destry ...
Epigenetics of Coeliac Disease
Epigenetics of Coeliac Disease

... Celiac Disease MEDICEL Istanbul 2012 ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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