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Gel Electrophoresis!
Gel Electrophoresis!

... • All cells have the same DNA • All multicellular organisms begin as a group of unspecialized cells = stem cells – No particular function ...
SBARS: fast creation of dotplots for DNA sequences on different
SBARS: fast creation of dotplots for DNA sequences on different

... amino acid sequences are based on algorithms working with text strings. Until recently, such methods were justified because the length of processed genetic text was relatively short. The evolution of sequencing techniques, however, has resulted in dramatically increased datasets, providing nucleotid ...
Honors Biology Module 7 Cellular Reproduction
Honors Biology Module 7 Cellular Reproduction

... mRNA, there are amino acids lined right up next to each other. So what is a protein? It is a bunch of amino acids linked together in a particular sequence. This happens again and again, so that many, many amino acids link up together. When all the amino acids called for by the codons on mRNA are lin ...
From DNA to Protein
From DNA to Protein

... URACIL (U) ...
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Document

... fluorescent in situ hybridization: (FISH) A technique used to identify the presence of specific chromosomes or chromosomal regions through hybridization (attachment) of fluorescently-labeled DNA probes to denatured chromosomal DNA. Step 1. Preparation of probe. A probe is a fluorescently-labeled seg ...
Chapter 10: Biotechnology
Chapter 10: Biotechnology

... • However, gene-by-gene analyses have many more practical benefits. • In fact, we have learned the function of many human genes by studying their counterparts in other species. • For example, researchers might learn the function of a certain gene in humans by disabling the expression of that same ge ...
LECTURE OUTLINE Cell Structure & Function DNA Replication
LECTURE OUTLINE Cell Structure & Function DNA Replication

... Wilkins, James Watson and Francis Crick publish their double-helix model DNA. ...
Structure of Nucleic Acids
Structure of Nucleic Acids

... molecule (that is, not involved in formation of a double helix), it can chemically attack the adjacent phosphodiester bond to cleave the backbone. RNA is transcribed with only four bases (adenine, cytosine, guanine and uracil),[9] but there are numerous modified bases and sugars in mature RNAs.Pseud ...
No Slide Title
No Slide Title

... Treat DNA with protease - transform Treat DNA with deoxyribonucleasesdestroy transforming activity ...
Manipulating DNA - Emerald Meadow Stables
Manipulating DNA - Emerald Meadow Stables

... produce large quantities of a desired protein • To work with genes in the laboratory, biologists often use bacterial plasmids, small, circular DNA molecules – Plasmids can: • can carry virtually any gene, • can act as vectors, DNA carriers that move genes from one cell to another, and • are ideal fo ...
Document
Document

... • Nucleolus - Site of ribosome production • Nucleus - location of DNA, cell organizer ...
Chapter 12: DNA & RNA
Chapter 12: DNA & RNA

... Uracil = Purple Amino Acid = Black Plastic Peptide Bonds (between AAs) – Grey Tubes 5. When you have constructed the complementary strand join the two strands together with Hydrogen bonds ...
Anatomy of a Virus
Anatomy of a Virus

... producing factory, the cell lyses and releases its products. • In a lysogenic cycle, the viral genome is incorporated into by genetic recombination into a specific site on the host cell’s chromosome. • It is now known as a prophage ...
Sample IHC Normal Expression Report Reason For Referral
Sample IHC Normal Expression Report Reason For Referral

... The results of the IHC analysis suggest the presence of normal DNA mismatch repair function within the tumor. Thus, the likelihood that this individual has an inherited colon cancer syndrome due to defective DNA mismatch repair (HNPCC/Lynch syndrome) is reduced but not eliminated. These results redu ...
AP Biology - Naber Biology
AP Biology - Naber Biology

... 47. Three types of RNA are needed for proteins synthesis. Complete the following chart. Type of RNA ...
7.03 Fall 2003 Problem Set #3 Solutions
7.03 Fall 2003 Problem Set #3 Solutions

... selecting for Kanr, you are selecting for cell that have successfully taken in the F plasmid, which confers resistance to kanamycin. If there merodiploids express b-gal normally, then you can conclude that the lac1- and lac3- mutations lie in different genes (they complement each other). If the mero ...
Spring Study Guide
Spring Study Guide

... How many daughter cells are produced? _________________________ 3. Which type of cell goes through meiosis? ____________________________ How many daughter cells are produced? ____________________________ How does the process of meiosis differ from that of mitosis? ____________________ ...
overexpression of mcm protein potentially causes cancer
overexpression of mcm protein potentially causes cancer

Appendix 11-Final examination of FOSC 4040 question
Appendix 11-Final examination of FOSC 4040 question

... (c) An individual may be heteroplasmic in one tissue and homoplasmic in another (d) All of the above (e) None of the above (44) Which of the following tests works better for samples that have undergone degradation? (a) STR typing (b) mtDNA typing (c) RFLP typing (d) none of the above (45) A lateral ...
Molecular Genetics And Otolaryngology
Molecular Genetics And Otolaryngology

... Detection of specific proteins is usually performed via Western Blot Analysis. This technique is similar to the Southern and Northern Blotting techniques used in DNA and RNA analysis. It involves the detection of specific protein extracts which are obtained via electrophoresis. Chromotography separa ...
Genetics in Epidemiology - University of Pittsburgh
Genetics in Epidemiology - University of Pittsburgh

... – Is a positive family history an independent risk factor for the disorder? • For many chronic disorders, a positive family history is associated with odds ratios between 2-6 ...
Genes that only humans have - Smurfit Institute of Genetics
Genes that only humans have - Smurfit Institute of Genetics

... 2006, several de novo genes were discovered in fruit flies. Since then, it’s become clear that genes do continually evolve in this way. Part of the explanation might be that biological systems are very noisy: even though most of our DNA is junk, most of it still gets transcribed into RNA at times, a ...
Alu-TPA PCR Kit (#8) Tech Service Training August ‘99
Alu-TPA PCR Kit (#8) Tech Service Training August ‘99

... • Segments of DNA which have the ability to move to or be copied to other regions of the genome Replicate are thought Element ...
Genomes and sequence alignment
Genomes and sequence alignment

... Transcriptomic sequence database management is hooey so far Amino acids Won't discuss today, but AA seqs. typically handled very differently and in different DBs Features: annotations, from location to function Loci are referred to as "features", which can be anything Genes, introns/exons, polymorph ...
Question How does DNA control a cell?By controlling Protein
Question How does DNA control a cell?By controlling Protein

... Where in the cell does transcription take place? ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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