• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Foundations of Biology.pptx
Foundations of Biology.pptx

... RNA splicing removes introns and splices exons together. Introns interrupt, but do not scramble, the DNA sequence that encodes a polypeptide. Sometimes, the separated exons code for different domains (functional regions) of the protein. Consensus sequences are short sequences between exons and intro ...
File
File

... Answer: No; after ligating the two DNAs together, the site (GGATCT or AGATCC) is a hybrid—BamH1-like on one end and BglII-like on the other. Therefore, neither enzyme will recognize this site. 3. Restriction endonucleases BamH1, NheI, XbaI, and EcoRI make sequence-specific cuts in DNA at the followi ...
3 - Hershey-Chase
3 - Hershey-Chase

... bacterium named __________________________, or ___________ for short and a virus called _____ that is a bacteriophage that infects E. coli When the new T2 viruses are ready to leave the host E. coli cell (and go infect others), they burst the E. coli cell open, killing it The results that Hershey an ...
virus - BiG.NeT
virus - BiG.NeT

... T=Tall tt= dwarf). List all possible combinations. Hint 9:3:3:1. ...
Combating Allergy and Asthma in Europe: Issues and Perspectives.
Combating Allergy and Asthma in Europe: Issues and Perspectives.

... of the bacterial 16S rRNA gene ...
Genetics notes, long version
Genetics notes, long version

... The order of P M A T steps, and what happens in the steps, is almost exactly the same as in Mitosis. BUT (Pictures and diagrams on 172-173 of your book) ...
Microbial Genetics
Microbial Genetics

... of the chromosome and begins a lytic stage, similar to that of a virulent phage. The virus replicates its DNA and protein, thousands of new virus particles are assembled, and the cell lyses to release the viruses. • Example: lambda (λ) phage of E. coli ...
FLASHCARDS
FLASHCARDS

... The synthesis of a protein from mRNA The tRNA complement to the mRNA codons CGAUUC The site of protein synthesis/translation The molecule that bears a codon The 2 attachments on a rRNA Change in DNA sequence Chemical or physical agent that interacts with DNA to cause a mutation The total # of chrom ...
Supplementary Material and Methods
Supplementary Material and Methods

... discriminate the translocated and the non-translocated BCL2 alleles in cases 1 and 2 we first sequenced the coding regions of the BCL2 gene in cases 1 and 2 using genomic DNA from whole tissue sections. In case 1 we found one SNP whereas no SNP was detected in case 2. We designed specific primers to ...
Defective de novo methylation of viral and cellular DNA sequences
Defective de novo methylation of viral and cellular DNA sequences

... DNA-binding proteins (HDACs, chromatin remodeling proteins) impaired and if yes -in what way? • Have all of the DNA methylating activities in mammalian cells been defined? ...
Nucleic Acids - Biology Junction
Nucleic Acids - Biology Junction

...  3 H bonds Matching bases? Why is this important? ...
DNA AP Bioloy
DNA AP Bioloy

...  3 H bonds Matching bases? Why is this important? ...
Bioinformatics 4 REtrieving DNA sequence
Bioinformatics 4 REtrieving DNA sequence

... clearly defined boundaries, and specific functional attributes. Furthermore, proteins of microbes or higher eukaryotes (animal and plants) have roughly the same properties. • The corresponding gene (DNA) sequences get more varied and complex in higher animals. Gene sizes in humans may vary from a fe ...
Answers to Mastering Concepts Questions
Answers to Mastering Concepts Questions

... and prepare for division in G2, but will not divide up its replicated DNA. A cell such as this, with too much DNA, would not be normal and would undergo apoptosis. 10. In the early 1900s, scientists began to experiment with radiation as a cancer treatment. Many physicians who administered the treatm ...
Amino Acids
Amino Acids

... • Speed up the rate of a reaction but they themselves do not change. ...
US Supreme Court Holds That Isolated Human DNA is Not Patent
US Supreme Court Holds That Isolated Human DNA is Not Patent

... known as complementary DNA (cDNA), “which contains the same protein-coding information found in a segment of natural DNA but omits portions within the DNA segment that do not code for proteins,” was patent eligible. Id. at **6. The Court found the latter to be patent eligible while the former was he ...
Chapter 10, 11, 12, 13 Review Questions
Chapter 10, 11, 12, 13 Review Questions

... A ladder; nucleotides; phosphate, sugar, A/T/C/G 3. Which nitrogen bases pair with each other? AT, TA CG, GC, AU 4. What is important about the way the letters are arranged? They must be in a certain order to produce the correct protein 5. How is DNA Replicated? What makes this a semi-conservative ...
Transcription Activity Guide
Transcription Activity Guide

... ...where molecules become real TM ...
Chapter 14 2015 - Franklin College
Chapter 14 2015 - Franklin College

... Why would chromosomes carry around extra DNA that isn’t used in the final mrna? A. Expensive to maintain (energy). B. Splicing out introns is a risky business (what if it’s done incorrectly) C. With these disadvantages, there must be an advantage or natural selection would not favor this arrangement ...
Genomic Maps and Linkage Analysis
Genomic Maps and Linkage Analysis

... pods (E) is dominant over squished (e). (a) A fully heterozygous plant has the dominant alleles linked in trans (i.e., dominant alleles not on the same homologue) at a map distance of 20 cM. What will be the genotypes of gametes produced by this plant, and in what frequencies ...
GENERAL PATHOLOGY Human Genetics
GENERAL PATHOLOGY Human Genetics

... instructions necessary to “tell” a living cell what it is supposed to do. there must exist a mechanism that copies DNA faithfully, to ensure that progeny cells contain the same genetic material as the maternal cell DNA replication or DNA synthesis is DNA replication or DNA synthesis is the process o ...
File
File

... Steps during Transcription • DNA unzips, exposing the strand that will code for the message • Free-floating nucleotides match up with the appropriate exposed base creating a strand of mRNA – what protein do you think brings the mRNA nucleotides to DNA? ...
Genetics - Region 11 Math And Science Teacher Partnership
Genetics - Region 11 Math And Science Teacher Partnership

... Its size is small. The adult worm is only 1 mm. This makes it possible to house large numbers in a laboratory setting. The total number of somatic cells in an adult worm is about 959 cells comparing to human's trillions of cells. Its life cycle/span is short (about 3 days/weeks respectively). This s ...
pdf
pdf

... a) 1 and 4 do not complement (the total number of phage is the same as the number of wild-type recombinants), 2 will complement 1, 3 and 4 (each pairwise co-infection gives 1010 total phage), and 3 will also complement all other mutants (1, 2 and 4). Thus mutants 1 and 4 are in the same complementat ...
Lesson Plan Title
Lesson Plan Title

... Description: This is document is a scaled down drawing of what the teacher will need to create on large white paper for each lab group. Step 3 ...
< 1 ... 378 379 380 381 382 383 384 385 386 ... 766 >

Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
  • studyres.com © 2026
  • DMCA
  • Privacy
  • Terms
  • Report