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GENETICS
GENETICS

... • Prokaryotic genetic material is organized in a simple circular structure that rests in the cytoplasm. • Eukaryotic genetic material is more complex and is divided into discrete units called genes. ...
Prokaryotes
Prokaryotes

... Make them more threatening, toxic, and resistant to antibiotics which prevent synthesis of peptidoglycan which inhibits cell wall growth ...
(lectures 26
(lectures 26

... functional loci. This degeneration of the Y was finally explained in 1978 by Brian Charlesworth, using the phenomenon of “Muller’s Ratchet”, which was invented by H. J. Muller (and explained more in 1974 by me, too). 13. Y chromosomes have no recombination through most of their length, for a simple ...
DNA Purity Instruments
DNA Purity Instruments

... Although broad spectrum xenon flash lamps have better stability than mercury lamps for DNA purity measurements, instruments with these lamps tend to be more expensive. Xenon flash lamps generate ample light across multiple wavelengths, but only light at a particular wavelength is useful for any sin ...
Exam1 2012 Life Student
Exam1 2012 Life Student

... ____ 88. After the DNA is replicated, and it condenses in prophase, two identical rods of DNA are seen. These are a. sister chromatids. b. centromeres. c. spindle fibers. d. kinetochores. e. chromatin. ____ 89. Spindle fibers a. are composed of DNA. b. only appear during interphase. c. attach to th ...
Eukaryotic Gene Expression Practice Problems Class Work 1
Eukaryotic Gene Expression Practice Problems Class Work 1

... 3. Transcription is the process of copying a sequence of DNA into a complementary strand of RNA. Number the events of transcription in order: ______RNA polymerase attaches at the promoter sequence on DNA ______ The new RNA strand separates from DNA. ______ RNA polymerase reaches a stop sequence and ...
lacI
lacI

... In bacterial RNA polymerse, the core enzyme consists of four subunits: two copies of alpha (α), a single copy of beta (β), and a single copy of beta prime (β’) ...
The Role of Ultrafiltration Membranes in the Recovery of DNA with
The Role of Ultrafiltration Membranes in the Recovery of DNA with

Document
Document

... Or better yet, sequence the entire transcriptome using RNASeq = Whole Transcriptome Shotgun Sequencing of all ...
Chemical organization of cells. Macromolecules
Chemical organization of cells. Macromolecules

... repeats – a region of dyad symmetry (fig. 16). In a double-strand DNA, the complementary sequences on one strand have the opportunity to base pair only if the strand separates from its partner. As a result a hairpin could be formed. The formation of two apposed hairpins creates a cruciform. Palindro ...
Eukaryotic Gene Expression Practice Problems Class Work 1
Eukaryotic Gene Expression Practice Problems Class Work 1

... 3. Transcription is the process of copying a sequence of DNA into a complementary strand of RNA. Number the events of transcription in order: ______RNA polymerase attaches at the promoter sequence on DNA ______ The new RNA strand separates from DNA. ______ RNA polymerase reaches a stop sequence and ...
Chapter 10 (Sample questions)
Chapter 10 (Sample questions)

... The sequence of bases on one strand of DNA could determine the a. sequence of bases in mRNA b. sequence of amino acids in a protein molecule c. sequence of bases in the other DNA strand d. all of the above choices are correct e. a and b are correct How does a cell use its DNA to create the cell itse ...
UNIT 9 NOTES Genetics
UNIT 9 NOTES Genetics

... there is only one replication origin (OriC). , Eukaryotic DNA is linear. When it is replicated, there are as many as 1000 replication origins. Despite these differences, however, the underlying process of replication is the same for both prokaryotic and eukaryotic DNA. (Both are double helical in na ...
Strain Improvement - Bharathiar University
Strain Improvement - Bharathiar University

... strain improvement of Aspergillus oryzae for protease production by both mutation and protoplast fusion.  UV radiation – 14 times higher yield.  Ethyl methanesulphonate – 39 times higher yield.  Protoplast fusion – using PEG and CaCl2 – 82 times higher yield. ...
43 ppt
43 ppt

... A.  aB  &  Ab  gametes  were  more  likely  than  AB  &  ab  gametes   B.  AB  &  ab  gametes  were  more  likely  than  aB  &  Ab  gametes     C.  aB,  Ab,  AB,  &  ab  gametes  were  equally  likely   D.  You  cannot  determin ...
Human Genetics and Populations: Chapters 14, 15 and 5 (mrk 2012)
Human Genetics and Populations: Chapters 14, 15 and 5 (mrk 2012)

... a. because it is difficult to insert new genes into them b. because they can be used to transform bacteria c. because they naturally contain much foreign DNA d. because they cannot be cut with restriction enzymes ____ 48. A plant cell is successfully transformed if a. a plasmid enters the cell and t ...
DNA repair - Journal of Cell Science
DNA repair - Journal of Cell Science

... NER consists of two subpathways: global genome repair (GGR), which removes damage in the genome overall and transcription-coupled repair (TCR), which specifically repairs the transcribed strand of active genes. The main difference between GGR and TCR is the requirement for different factors during t ...
Protein Synthesis
Protein Synthesis

... • RNA is read three Nitrogen bases at a time by a molecule to code for one amino acid. • The three-nucleotide sequence of RNA is called a codon. • Each 3-nucleotide codon codes for a specific amino acid. • A codon chart is used to find what amino acid each codon codes for. ...
Here
Here

... one of the two genes. The same goes for the genes for vulnerability to drought and to resist a pesticide This means that the offspring has a ...
Chapter 16.
Chapter 16.

... The “Central Dogma”  flow of genetic information within a cell ...
Genetic Engineering: How and why scientists manipulate DNA in
Genetic Engineering: How and why scientists manipulate DNA in

... If unknown is heterozygous, some will show dominant trait, some show recessive trait Complete Problem Solving Lab 13.1 p.339 ...
Dow Agrosciences Australia - PDF 170 KB
Dow Agrosciences Australia - PDF 170 KB

... EXZACT™ Delete technique is used to delete undesirable DNA sequences to improve specific traits from the plant genome. EXZACT™ Edit allows scientists to re-write gene sequences to improve specific traits, while EXZACT™ Add allows a single gene sequence or a series of gene sequences (stack) to be add ...
Sequencing a genome and Basic Sequence Alignment
Sequencing a genome and Basic Sequence Alignment

... The figure shows to sequences of nucleic acids. Some have the same base (nucleic acid ) and so there is a match at this position between the strands. This is represented by a vertical line and a blue highlight. Others do not match and have no vertical line and blue highlight: these are unmatched pai ...
Cybergenetics TrueAllele Technology Enables
Cybergenetics TrueAllele Technology Enables

DNA Transcription and Translation
DNA Transcription and Translation

... Gene Regulation: ability of an organism to control which genes are transcribed. ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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