double core - MG University
... 22. In vitro mutagenesis 23. Nick translation 24. Homopolymer tailing Part C (Answer any 4- weight 2 each) 26. What are the advantages of using a restriction enzyme with relatively few cutting sites? When would you use such enzymes? 25. The human insulin gene contains a number of introns. In spite o ...
... 22. In vitro mutagenesis 23. Nick translation 24. Homopolymer tailing Part C (Answer any 4- weight 2 each) 26. What are the advantages of using a restriction enzyme with relatively few cutting sites? When would you use such enzymes? 25. The human insulin gene contains a number of introns. In spite o ...
Tutorial - Faster Better Media
... most PCR and RT-PCR products. Use high voltage (350-450 volts for a 10 cm gel, or 35-45 V/cm). 2% gels show the sub-500 bp region exceptionally well. Use a standard agarose. •Employ a high-strength agarose to permit use of 0.3 to 0.5% agarose to produce better separation of DNA fragments larger than ...
... most PCR and RT-PCR products. Use high voltage (350-450 volts for a 10 cm gel, or 35-45 V/cm). 2% gels show the sub-500 bp region exceptionally well. Use a standard agarose. •Employ a high-strength agarose to permit use of 0.3 to 0.5% agarose to produce better separation of DNA fragments larger than ...
Structure of DNA (Deoxyribonucleic acid)
... • 2nd tRNA attaches to second binding site on ribosome; amino acids are joined by peptide bond • Ribosome moves forward; subsequent tRNA molecules bring amino acids to ribosome and are joined by peptide bonds • Process continues until stop codon is reached Picture of translation ...
... • 2nd tRNA attaches to second binding site on ribosome; amino acids are joined by peptide bond • Ribosome moves forward; subsequent tRNA molecules bring amino acids to ribosome and are joined by peptide bonds • Process continues until stop codon is reached Picture of translation ...
File
... have no polypeptide products but play crucial roles in the cell. Thus, we arrive at the following definition: A gene is a region of DNA that can be expressed to produce a final functional product that is either a polypeptide or an RNA molecule. When considering phenotypes, however, it is useful to f ...
... have no polypeptide products but play crucial roles in the cell. Thus, we arrive at the following definition: A gene is a region of DNA that can be expressed to produce a final functional product that is either a polypeptide or an RNA molecule. When considering phenotypes, however, it is useful to f ...
Biotech & Genetic Engineering PP
... Biotechnology is used to identify people, produce transgenic organisms and clones, study diseases and evolution, and create medical treatments for people with ...
... Biotechnology is used to identify people, produce transgenic organisms and clones, study diseases and evolution, and create medical treatments for people with ...
Cell Division Mitosis vs. Meiosis - kromko
... Important Note: Each amino acid is joined the correct tRNA molecule by a specific enzyme. This process requires energy in the form of ATP. 2.) Elongation: Amino acids are added to the growing polypeptide one at a time. • Codon recognition – tRNA anticodon pairs with mRNA codon at the A site. • Pepti ...
... Important Note: Each amino acid is joined the correct tRNA molecule by a specific enzyme. This process requires energy in the form of ATP. 2.) Elongation: Amino acids are added to the growing polypeptide one at a time. • Codon recognition – tRNA anticodon pairs with mRNA codon at the A site. • Pepti ...
pptx format
... Different organisms diverse from each other by the sequence of the basic breaks and their number. ...
... Different organisms diverse from each other by the sequence of the basic breaks and their number. ...
Protein Synthesis
... cytoplasm. One problem however. DNA cannot be moved from the nucleus. There must be a process that allows for the information from the DNA in the nucleus to be transferred to the ribosomes in the cytoplasm. Transcription Transcription is defined as the process in which the genetic information (genes ...
... cytoplasm. One problem however. DNA cannot be moved from the nucleus. There must be a process that allows for the information from the DNA in the nucleus to be transferred to the ribosomes in the cytoplasm. Transcription Transcription is defined as the process in which the genetic information (genes ...
DNA Technology
... 12.11 The analysis of genetic markers can produce a DNA profile DNA profiling is the analysis of DNA fragments to determine whether they come from the same individual. DNA profiling – compares genetic markers from noncoding regions that show variation between individuals and ...
... 12.11 The analysis of genetic markers can produce a DNA profile DNA profiling is the analysis of DNA fragments to determine whether they come from the same individual. DNA profiling – compares genetic markers from noncoding regions that show variation between individuals and ...
Solutions to Genetics Day 6 Interpretation Questions
... On day 4, the goal was to move the gene carrying the insertion mutation into a new bacterial strain. How was the random insertion of DNA into the bacterial genome accomplished? Name one thing that could have prevented this from occurring. We used a modified λ phage that carried the mini-TN10 transpo ...
... On day 4, the goal was to move the gene carrying the insertion mutation into a new bacterial strain. How was the random insertion of DNA into the bacterial genome accomplished? Name one thing that could have prevented this from occurring. We used a modified λ phage that carried the mini-TN10 transpo ...
DNA polymerase - yusronsugiarto
... original DNA: has one end defined by the primer, but the other end is not well defined. Copy number grows linearly. • all other PCR products have 2 ends defined by the primers, so they have a constant length and can be easily detected by electrophoresis. Copy number grows exponentially. ...
... original DNA: has one end defined by the primer, but the other end is not well defined. Copy number grows linearly. • all other PCR products have 2 ends defined by the primers, so they have a constant length and can be easily detected by electrophoresis. Copy number grows exponentially. ...
Chapter 25 Molecular Basis of Inheritance
... proteins - small subunits contains 1 rRNA molecule and 21 proteins - large subunits contains 2 rRNA molecules and 34 proteins - includes enzymes that form peptide bonds between amino acids - attach and move along mRNA to decide the amino acid sequence for a protein - several ribosomes (a polyribosom ...
... proteins - small subunits contains 1 rRNA molecule and 21 proteins - large subunits contains 2 rRNA molecules and 34 proteins - includes enzymes that form peptide bonds between amino acids - attach and move along mRNA to decide the amino acid sequence for a protein - several ribosomes (a polyribosom ...
9.1 Manipulating DNA - SBI4u Biology Resources
... In humans, methyl groups are used to tag genes to turn them on or off. Stay tuned. ...
... In humans, methyl groups are used to tag genes to turn them on or off. Stay tuned. ...
1. The Building Blocks of DNA
... the molecule. The sugar-phosphate bonds are called phosphodiester bonds. The carbons of the sugar groups are numbered 1’ through 5’ (next slide). One part of the phosphodiester bond is between the phosphate and the 5’ carbon of deoxyribose, and the other is between the phosphate and the 3’ carbon of ...
... the molecule. The sugar-phosphate bonds are called phosphodiester bonds. The carbons of the sugar groups are numbered 1’ through 5’ (next slide). One part of the phosphodiester bond is between the phosphate and the 5’ carbon of deoxyribose, and the other is between the phosphate and the 3’ carbon of ...
DNA! - Chapter 10
... topoisomerase, RNase H, and ligase play in DNA replication? 3. What is the difference between how the leading strand and lagging strand are copied during DNA replication? Why do they have to be synthesized differently in this fashion? 4. What would happen if insufficient RNase H were produced by a c ...
... topoisomerase, RNase H, and ligase play in DNA replication? 3. What is the difference between how the leading strand and lagging strand are copied during DNA replication? Why do they have to be synthesized differently in this fashion? 4. What would happen if insufficient RNase H were produced by a c ...
Cell Cycle, Cancer, and the Biology Student Workbench
... In looking for a mutation, they should be very similar with only a few changes. For this activity choose tumor protein p53 (Li-Fraumeni syndrome)... Check it and import the sequence. ...
... In looking for a mutation, they should be very similar with only a few changes. For this activity choose tumor protein p53 (Li-Fraumeni syndrome)... Check it and import the sequence. ...
Molecular Genetics
... B. The Role of Ribosomal RNA 1. Ribosomal RNA (rRNA) is produced from a DNA template in the nucleolus of the nucleus. 2. The rRNA is packaged with a variety of proteins into ribosomal subunits, one larger than the other. 3. Subunits move separately through nuclear envelope pores into the cytoplasm w ...
... B. The Role of Ribosomal RNA 1. Ribosomal RNA (rRNA) is produced from a DNA template in the nucleolus of the nucleus. 2. The rRNA is packaged with a variety of proteins into ribosomal subunits, one larger than the other. 3. Subunits move separately through nuclear envelope pores into the cytoplasm w ...
CHAPTER 18 OBJECTIVES-BACTERIAL GENOME The Genetics of
... 1. Explain how advances in recombinant DNA technology have helped scientists study the eukaryotic genome. 2. Describe the natural function of restriction enzymes and explain how they are used in recombinant DNA technology. 3. Explain how the creation of sticky ends by restriction enzymes is useful i ...
... 1. Explain how advances in recombinant DNA technology have helped scientists study the eukaryotic genome. 2. Describe the natural function of restriction enzymes and explain how they are used in recombinant DNA technology. 3. Explain how the creation of sticky ends by restriction enzymes is useful i ...
View/Open - Technical University of Mombasa
... f) Explain the possible forms of tyrosine that could result due to the degeneracy of the genetic code. (5marks) Question TWO a) Describe the biological significance of various types of plasmids ...
... f) Explain the possible forms of tyrosine that could result due to the degeneracy of the genetic code. (5marks) Question TWO a) Describe the biological significance of various types of plasmids ...
Document
... survival will not adequately predict invasiveness. Variation in the competitive environment and timing of introductions can confound predictions. Unknown factors cause unexplained time lags that occur between the introduction of the species and the expansion of its population. These represent key ch ...
... survival will not adequately predict invasiveness. Variation in the competitive environment and timing of introductions can confound predictions. Unknown factors cause unexplained time lags that occur between the introduction of the species and the expansion of its population. These represent key ch ...
Cre-Lox recombination
In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.