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Oct26 - Staff Web Pages
Oct26 - Staff Web Pages

... In translation, the mRNA strand created in transcription is read and translated into a protein. The mRNA binds to a ribosome, a large organelle found in the cytoplasm. The strand of mRNA is pulled through the ribosome three bases at a time. Each of these triplets on the mRNA strand is called a codon ...
Brouwer_791H_Proposal - University of New Hampshire
Brouwer_791H_Proposal - University of New Hampshire

... a phosphate group (PO42-). There are four potential nitrogenous bases in DNA; adenine, guanine, cytosine, and thymine. It is the pattern of these four bases that ...
transcription and translation
transcription and translation

... Central Dogma- DNARNAProtein ...
Station 1
Station 1

... different amino acid than the original sequence did. In addition, a single adenine (A) base ends the strand. This frame-shift mutation will cause massive changes in the types of protein produced by the new strand. ...
Chapt21 Lecture 13ed Pt 4
Chapt21 Lecture 13ed Pt 4

... Biotechnology products: Transgenic animals • Gene is inserted into the egg that when fertilized will develop into a transgenic animal • Current uses – ____________: production of pharmaceuticals in the milk of farm animals – ____________: includes fish, cows, pigs, rabbits, and sheep – Mouse models: ...
Biology 212 General Genetics
Biology 212 General Genetics

... Compare the genotype of the most frequent class to the least frequent class and determine which marker changes places. ...
Genomics and Behavior “Central Dogma” Outline
Genomics and Behavior “Central Dogma” Outline

... nucleus to the ribosome • Protein synthesis takes place based on the genetic code • A three base codon codes for an amino acid ...
CONTENTS DNA, RNA AND PROTEIN SYNTHESIS DNA
CONTENTS DNA, RNA AND PROTEIN SYNTHESIS DNA

... synthesize both strands simultaneously. A portion of the double helix must first unwind, and this is mediated by helicase enzymes. The leading strand is synthesized continuously but the opposite strand is copied in short bursts of about 1000 bases, as the lagging strand template becomes available. T ...
Nucleic Acid Chemistry
Nucleic Acid Chemistry

... • Made of protein and rRNA • 2 subunits • Has internal sites for 2 transfer RNA molecules ...
Chapter Outline
Chapter Outline

... additional changes before it is biologically functional. b. Bovine proinsulin, for example, is inactive when first produced; a single long polypeptide folds into a three-dimensional structure, a sequence of 30 amino acids is removed from the middle, and the two polypeptide chains are bonded together ...
File
File

Nucleic Acids-Structure, Central Dogma
Nucleic Acids-Structure, Central Dogma

...  SSB (single-stranded DNA-binding proteins) – binds to the unwound strands, preventing re-annealing ...
Cat Coat Color Genetics Part 2: Coat Patterns
Cat Coat Color Genetics Part 2: Coat Patterns

... “mink” color, that is the Siamese pattern, but with a darker body color. Cats containing only one variant gene are known as a carriers, and will not have the classic points color pattern (for example C cs ). The mating of two cats that each have two copies of the variant gene (ie cs cs) results in a ...
It is essential for students to understand
It is essential for students to understand

... the mutation will not be passed to the offspring of the organism. If the mutant cell is a gamete (sex cell), the altered DNA will be transmitted to the embryo and may be passed to subsequent generations. Gamete cell mutations can result in genetic disorders. nondisjunction ...
Recombinant DNA Lab
Recombinant DNA Lab

... sequence. The result is a set of double-stranded DNA fragments with single-stranded ends, called "sticky ends." Sticky ends are not really sticky; however, the bases on the single stranded ends do easily form base pairs with the complementary bases on other DNA molecules. Thus, the sticky ends of DN ...
Lecture: How do neurons work
Lecture: How do neurons work

... Restriction enzymes, also called restriction nucleases (EcoRI in this example), surround the DNA molecule at the point they seek (sequence GAATTC). They cut one strand of the DNA double helix at one point and the second strand at a different, complementary point (between the G and the A base). The s ...
DNA : The Genetic Material
DNA : The Genetic Material

... • 3. Free floating nucleotides join with the complementary nucleotides on the single strands. • 4. DNA polymerase (enzyme) binds to the separated chain and links the nucleotide back into a long strand. • DNA polymerase also has a proof reading role- in the event of a mismatched nucleotide it can rep ...
DNA Isolation: plant materials
DNA Isolation: plant materials

... The purpose of this lab is to give students the opportunity to extract, observe, and analyze DNA. These are all fundamental skills involved in Biotechnology. The DNA in a cell is about 100,000 times as long as the cell itself. However, DNA only takes up about 10% of the cell's volume. This is becaus ...
Activity: Can You Crack the Code
Activity: Can You Crack the Code

... Chromosomes are composed mostly of DNA. A DNA molecule is made up of four different nitrogen bases – adenine (A), thymine (T), guanine (G), and cytosine (C). These bases form the rungs of the DNA “ladder.” A single gene on a chromosome may contain anywhere from several hundred to a million or more o ...
Protein
Protein

... Gene Regulation: ability of an organism to control which genes are transcribed. ...
File
File

... Demonstrate that changes in genotype causes changes in phenotype by transforming E.coli into fluorescent ...
Cells and DNA Table of Contents
Cells and DNA Table of Contents

... Every person has two copies of each gene, one inherited from each parent. Most genes are the same in all people, but a small number of genes (less than 1 percent of the total) are slightly different between people. Alleles are forms of the same gene with small differences in their sequence of DNA ba ...
Finding Genes in Eukaryotes
Finding Genes in Eukaryotes

... Usually the primary challenge that follows the sequencing of anything from a small segment of DNA to a complete genome is to establish where the various functional elements such as genes, promoters, terminators etc., lie in the sequence. This module concentrates on the identification of regions of D ...
Document
Document

... good choice for cutting out the insulin gene from the human DNA by verifying that it meets all the following criteria: • It does not cut within the insulin gene • It cuts very close to the beginning and end of the gene • It will allow the insulin gene to be inserted into the cut plasmid 6. Use sciss ...
12.3 RNA and Protein Synthesis
12.3 RNA and Protein Synthesis

... • Messenger RNA (mRNA) carries copies of messages encoded on DNA to the rest of the cell. • Ribosomal RNA (rRNA) makes up part of the ribosome ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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