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Plasmids by Dr. Ty C.M. Hoffman
Plasmids by Dr. Ty C.M. Hoffman

... Plasmids  occur  naturally,  but  they  can  also  be  used  in  biotechnological  applications.  With  modern   technology,  plasmids  can  be  engineered  to  contain  any  gene  of  interest.  Genes  can  be  inserted  into  a   plasmi ...
Chromosomes - life.illinois.edu
Chromosomes - life.illinois.edu

Document
Document

Cell Cycle, DNA, and Protein Synthesis
Cell Cycle, DNA, and Protein Synthesis

... no growth factor is present. • Cancer begins as a single cell • This cell is normally found and destroyed by the body’s immune system. If not, this cell could divide into a mass of identical daughter cancer cells that: – Impair the function of one or more organs – malignant tumor • Cells can break o ...
Chapter 10
Chapter 10

... resulting in a hybrid molecule that can be transferred to a host cell, and get replicated in it ...
Intro to Genetics
Intro to Genetics

... If the answer is false, change crucial information in the statement to make it true. _____11. Rosalind Franklin discovered jumping genes. _____12. Sex influenced traits are usually autosomal. _____13. Height is both a polygenic and a complex character. _____14. A somatic cell mutation is passed on t ...
Evolutionary Anthropology
Evolutionary Anthropology

... hydrogen bonds, i.e. can be unzipped for DNA replication or RNA transcription  DNA replication - Reliably replicates message by unzipping and using singlestranded template to synthesize new DNA  RNA transcription – Again unzips DNA and uses single-stranded DNA template to synthesize RNA for protei ...
RNA nucleotides
RNA nucleotides

... rRNA (Ramada Inn) combines with protein to become apart of the ribosome. mRNA and tRNA will arrive at this ribosome. mRNA (Lil Momma) contains codons (eggs) that are complementary to tRNA’s (Tyrone) ...
BIO2093_DMS3_phylogeny - COGEME Phytopathogenic Fungi
BIO2093_DMS3_phylogeny - COGEME Phytopathogenic Fungi

... represented as phylogenetic trees based on alignment of protein sequences. • Gene duplication and loss can be inferred from phylogenetic trees. • Protein sequences evolve more slowly than DNA sequences (due to redundancy in genetic code). ...
No Slide Title
No Slide Title

... 2. Somatic mutations - occur in other body cells. These are not inheritable (cancer) Gene Mutations – involve changes in the nucleotides. Types of gene mutations: point mutation – one nucleotide affected Frameshift mutation – deleted or inserted nucleotide Chromosome mutations - when there is a chan ...
AP Biology Fall Semester Review
AP Biology Fall Semester Review

... 11) From each primary oocyte that undergoes meiosis, the number of functional egg cells produced is a. one b. two c. three d. four e. eight 12) If the following events in the formation of the synaptonemal complex and crossing over were arranged in order, which event would be third? a. Homologous chr ...
CF29v2 - DNA testing in Cystic Fibrosis - Bio
CF29v2 - DNA testing in Cystic Fibrosis - Bio

Mendelian Genetics - Home | Phoenix College
Mendelian Genetics - Home | Phoenix College

... – For each trait there is 1 gene – Gene exists in 2 forms called alleles – Each parent can only give one allele to their ...
university of oslo
university of oslo

... U6), plus a few auxiliary proteins, involved in intron splicing. The process starts with cleavage of the 5’ splice site by a transesterification reaction that links the 5’ end of the intron to a specific adenine nucleotide in the intron sequence. A second transesterification reaction links the 5’ ph ...
dsRNA synthesis RNAi (Howard Clarke)
dsRNA synthesis RNAi (Howard Clarke)

... Selection and preparation of DNA template: Chose an exon-rich region of genomic DNA 300bp in length (>500 is better, and 3’ UTR sequence is fine). Alternatively, cDNA clones or first-strand cDNA generated by RT-PCR can be used as template (see protocol “Oligo d(T) primed cDNA synthesis”). cDNA templ ...
PPT poster - Davidson College
PPT poster - Davidson College

... Cell Division ...
23 development of molecular markers to distinguish cytoplasm
23 development of molecular markers to distinguish cytoplasm

... fragment also yielded two groups, separating the D8 and B1 lines from the remaining alloplasms in the study. The identity of these lines was confirmed. The low level of polymorphisms found among the cytoplasms in relation to the number of primers used and digestions performed can be explained by the ...
Lecture 35: Basics of DNA Cloning-I
Lecture 35: Basics of DNA Cloning-I

... recombinant colonies. Vector with desired DNA insert is called recombinant DNA. This can be transferred to suitable host system (generally E.Coli) where it finds machinery for replication and makes several copies of it (may also express protein). The process is also called recombinant DNA technology ...
GENETICS 603 EXAM 1 Part 1: Closed book October 3, 2014 NAME
GENETICS 603 EXAM 1 Part 1: Closed book October 3, 2014 NAME

... base  changes  induced  than  those  included  in  the  test.  This  was  true  even  though  they  were   single  base-­‐pair  point  mutations,  including  nonsense  mutations  that  fully  eliminated   enzyme  activity.  Suggest  an  exp ...
ECEN Information theory for genetics
ECEN Information theory for genetics

... (2) Campus policy regarding religious observances requires that faculty make every effort to reasonably and fairly deal with all students who, because of religious obligations, have conflicts with scheduled exams, assignments or required attendance. In this class, {{insert your procedures here}} See ...
Chapter 2 Evolution, Genetics, and Experience
Chapter 2 Evolution, Genetics, and Experience

different plant species - Bio
different plant species - Bio

... The QuickPick SML Plant DNA purification kit provides a fast and simple means of purifying genomic DNA from a variety of plants or their organelles. The technique does not require any organic solvents and eliminates the need for repeated centrifugation, vacuum filtration or column separation. The re ...
Branching in DNA Computation
Branching in DNA Computation

... Works in parallel checking all the solutions at once If the solution is not valid (the variables in it contradict each other and thus the clause can not be resolved) then folds on itself An if statement acts on each clause at the same time! Disadvantages: – high error rates (esp. with increase of va ...
AP Biology - APBioKorzwiki
AP Biology - APBioKorzwiki

... gel electrophoresis Southern blotting microarrays ...
Answers to End-of-Chapter Questions – Brooker et al ARIS site
Answers to End-of-Chapter Questions – Brooker et al ARIS site

< 1 ... 389 390 391 392 393 394 395 396 397 ... 766 >

Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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