• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

... 12. What is Edman’s reagent? Describe its use in the determination of amino acid sequence in proteins. 13. Explain the Michaelis theory of enzyme catalysis. 14. Give the classification of lipids. Mention its biological importance. 15. Explain the types of plasma lipoproteins. 16. What are nucleic ac ...
Document
Document

... • Four ______________ sex cells have been formed from one original _____________ cell. • Each haploid cell contains one ____________________ from each homologous pair. • Haploid cells become ______________, transmitting the genes they contain to offspring. ...
Answer Key to Chapter 10 Reading
Answer Key to Chapter 10 Reading

... Where a bacterium takes up DNA from its environment ...
Scientific Writing
Scientific Writing

... 1) It should be relatively simple (e.g. not involving multiple steps such as attachment of a ligand targeting a particular cell type) & result in high vector concentrations (>108 particles/ml). 2) To allow subsequent readministration & avoid undesired host reactions there would be no significant imm ...
DNA cloning
DNA cloning

... Concept 20.1: DNA cloning yields multiple copies of a gene or other DNA segment • To work directly with specific genes, scientists prepare well-defined segments of DNA in identical copies, a process called DNA cloning ...
Microbial Genetics
Microbial Genetics

Class Topics - Seneca High School
Class Topics - Seneca High School

... – The order and number of amino acids determines the protein’s properties – DNA determines the order of amino acids because it’s the template Page: 6 ...
Chapter 22 & 23
Chapter 22 & 23

... into viral DNA, then the virus will insert the therapeutic gene into the cell’s DNA as well • As a result, the new cell will have a functional gene that has replaced the damaged gene • In theory, if germ-line cells were targeted by these viruses, then modifications could be passed on to the next ...
Assessment questions and LO`s for each section
Assessment questions and LO`s for each section

... List the steps/stages of an immune response Highlight the step that is blocked by HIV What is the function of Helper T-Cells? What does the macrophage do? What “activates” the Helper T-Cell? What does the B-Cell do? How does the B-Cell react to an activated Helper T-Cell? How do antibodies work to n ...
workshop-1
workshop-1

... Also take a statistical approach: - coding and non-coding sequence are slightly different in composition - some ‘possible’ splice sites are more likely than others scan genomic sequence … . . .CGTCGTATGGCTTCGATGTAGTACATCGGATCGGTATGGAATCATTTCAGTCGCTAGCTAGCCTAACGTATATAGCTAGGTAAGACTA. . ...
DNA Questions #4 Questions on the PCR Process:
DNA Questions #4 Questions on the PCR Process:

... __greater___________ (greater, less) the value in DNA fingerprinting. (There are two different answers heresmaller, greater and larger, less.) Explain why: _____Like identifying people in a room, it is better to use a characteristic like, “wears pink colored glasses” (not as common or frequent) than ...
Recombinant DNA and the Production of Insulin
Recombinant DNA and the Production of Insulin

... Recombinant DNA and the Production of Insulin Part One Diabetes is a condition where a person has too much sugar in their blood. Insulin, which is a hormone created by the pancreas, normally helps lower the level of sugar in a person’s blood. But people who are diabetics do not produce enough insuli ...
Titan Tutoring for Biology
Titan Tutoring for Biology

... proved invalid by the work of several important scientists, and it has been replaced by the theory that all living things come from other living things. This theory is called biogenesis. ...
Chapter 20: Biotechnology
Chapter 20: Biotechnology

... One indirect method of rapidly analyzing and comparing genomes is gel electrophoresis This technique uses a gel as a molecular sieve to separate nucleic acids or proteins by size A current is applied that causes charged molecules to move through the gel Molecules are sorted into “bands” by their siz ...
Chapter 15
Chapter 15

... 3. Crossing Over – sections of chromosomes switching places - genetic recombination – new traits in offspring - parent types – offspring like parents - recombinant – offspring w/ new traits - a 50% frequency of recombination is observed for 2 genes on different chromosomes - If Morgan’s flies were c ...
Nucleic Acids and DNA
Nucleic Acids and DNA

... • Watson and Crick took this data and other material that hinted that DNA stacked to propose that DNA was double stranded. ...
You Light Up My Life
You Light Up My Life

... play in determining the primary structure of polypeptide chains during translation. ...
Pre – AP Biology
Pre – AP Biology

... molecule back into a DNA molecule. Insert the new DNA strand into bacteria. The bacteria will then be able to Transcribe and Translate off of this new inserted DNA and thus make that protein. This has been done for numerous human medicines such as Insulin or Human Growth Hormone. – Eukaryotes DO hav ...
Grant IGA MZČR 8563-5/2005 Genetický profilů genů metabolismu
Grant IGA MZČR 8563-5/2005 Genetický profilů genů metabolismu

... Chapter 16: Cancer genetics and genomics: Oncogenes, Tumorsuppressor genes (including Retinoblastoma,Caretaker genes in autosomal recessive chromosome instability syndromes, Cytogenetic changes in cancer, Gene amplification) Chapter 6: Principles of clinical cytogenetics:Mendelian disorders with cyt ...
5 DNA History Replication
5 DNA History Replication

... Transformation = change in phenotype something in heat-killed bacteria could still transmit AP Biology disease-causing properties ...
Crossing Over during Meiosis
Crossing Over during Meiosis

... pairs will lead (eventually) to gene maps of each chromosome. • Pair-wise and three-locus linkage associations can be formed. • The frequencies of recombination can also be used to estimate the physical distance between loci along a chromosome. • The values for recombination frequency can be conside ...
7.014 Quiz III Handout
7.014 Quiz III Handout

... of the Agrobacterium genes are found on its large chromosome, but some of the genes that allow this bacterium to infect plant cells are found on the bacterial Ti plasmid. During infection, the bacterium transfers the Ti plasmid DNA to the plant and the plasmid DNA is integrated into the genome of th ...
DNA: Pandora`s Box Questions
DNA: Pandora`s Box Questions

DNA replication - Olympic High School
DNA replication - Olympic High School

12.6 DNA Repair
12.6 DNA Repair

... Pyrimidine dimers - bonds between C’s and/or T’s on the same strand.  Photolyases - enzymes that absorb light energy and use it to detect and bind to pyrimidine dimers, then break the extra bond.  Humans do not have this type of repair ...
< 1 ... 392 393 394 395 396 397 398 399 400 ... 766 >

Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
  • studyres.com © 2026
  • DMCA
  • Privacy
  • Terms
  • Report