B. Sc. Part- II (GENETICS)
... Note: Question 1 will be compulsory and short answer type covering entire syllabi. Four questions will be set from each Section. Candidates have to answer five questions in all selecting two from each section. SECTION- A I. GENETIC MATERIAL: Evidence to prove that DNA is the genetic material, its st ...
... Note: Question 1 will be compulsory and short answer type covering entire syllabi. Four questions will be set from each Section. Candidates have to answer five questions in all selecting two from each section. SECTION- A I. GENETIC MATERIAL: Evidence to prove that DNA is the genetic material, its st ...
No Slide Title
... is that foreign genes can be over-expressed, due to the high gene copy number, up to 100 000 compared with single-copy nuclear genes. And there does not seem to be gene-silencing and other instability that plague nuclear transformation. The gene product is retained inside the chloroplasts or can in ...
... is that foreign genes can be over-expressed, due to the high gene copy number, up to 100 000 compared with single-copy nuclear genes. And there does not seem to be gene-silencing and other instability that plague nuclear transformation. The gene product is retained inside the chloroplasts or can in ...
Techniques
... • Early procedure – cut tissue into very thin sections, adhere to microscope slides. Use a probe to look for mRNA directly in tissue. ...
... • Early procedure – cut tissue into very thin sections, adhere to microscope slides. Use a probe to look for mRNA directly in tissue. ...
Gene targeting (contd)
... • Why would we want to do this anyway? – Determine function of identified genes – develop animal models for various diseases – confer desirable property • Choice of method depends on model system, developmental stage and outcome desired – early embryos • if cells are large than direct microinjection ...
... • Why would we want to do this anyway? – Determine function of identified genes – develop animal models for various diseases – confer desirable property • Choice of method depends on model system, developmental stage and outcome desired – early embryos • if cells are large than direct microinjection ...
DNA Replication
... single high density band - all DNA molecules contained the "heavy" nitrogen. ･DNA taken from the two generations after the switch contained an intermediate-density band - DNA contained a "heavy" DNA strand from the parent and a complementary "light" DNA strand. ･Density results from generation 3, di ...
... single high density band - all DNA molecules contained the "heavy" nitrogen. ･DNA taken from the two generations after the switch contained an intermediate-density band - DNA contained a "heavy" DNA strand from the parent and a complementary "light" DNA strand. ･Density results from generation 3, di ...
DNA
... generalized transduction: A DNA fragment is transferred from one bacterium to another by a lytic bacteriophage that is now carrying donor bacterial DNA due to an error in maturation during the lytic life cycle. specialized transduction: A DNA fragment is transferred from one bacterium to another b ...
... generalized transduction: A DNA fragment is transferred from one bacterium to another by a lytic bacteriophage that is now carrying donor bacterial DNA due to an error in maturation during the lytic life cycle. specialized transduction: A DNA fragment is transferred from one bacterium to another b ...
Genetic Vulnerability Factors - Early Psychosis Intervention
... DNA stands for deoxyribonucleic acid. This complicated name just describes the chemical structure of the molecule. DNA is made up 4 types of smaller unit called nucleotides. These 4 types of DNA unit (the nucleotides) each have a letter, they are A C G and T. The DNA in each cell of our bodies conta ...
... DNA stands for deoxyribonucleic acid. This complicated name just describes the chemical structure of the molecule. DNA is made up 4 types of smaller unit called nucleotides. These 4 types of DNA unit (the nucleotides) each have a letter, they are A C G and T. The DNA in each cell of our bodies conta ...
Name
... The number and location of bones in many fossil vertebrates is similar to living vertebrates. How would biologist explain this fact? __________________________________________________________________ ...
... The number and location of bones in many fossil vertebrates is similar to living vertebrates. How would biologist explain this fact? __________________________________________________________________ ...
Document
... Scalable purification of archive-quality DNA from 100 to 5 x 108 cultured cells using the Gentra® Puregene® Cell Kit This protocol provides information about scaling of reagents required for purification of DNA from 100 to 5 x 108 cultured cells using the Gentra Puregene Cell Kit. The Gentra Puregen ...
... Scalable purification of archive-quality DNA from 100 to 5 x 108 cultured cells using the Gentra® Puregene® Cell Kit This protocol provides information about scaling of reagents required for purification of DNA from 100 to 5 x 108 cultured cells using the Gentra Puregene Cell Kit. The Gentra Puregen ...
Schedule of Lecture and Laboratory Sessions
... 23. To discuss problems in gene therapy 24. To explain the mechanism of transformation and view aspects of plasmids including ori, ampr, plasmid size, extrachromosomal maintained, and the multiple cloning sites for the insertion of foreign genes 25. To examine the pGLO plasmid, ori, ampr ,the GFP ge ...
... 23. To discuss problems in gene therapy 24. To explain the mechanism of transformation and view aspects of plasmids including ori, ampr, plasmid size, extrachromosomal maintained, and the multiple cloning sites for the insertion of foreign genes 25. To examine the pGLO plasmid, ori, ampr ,the GFP ge ...
Recombination Chromosome Separations At Anaphase I And II
... To determine the proportion of equational separations at a locus after "n” chiasmata: En=2/3[1 - (-1/2)n] (Table 1 and page 57) ...
... To determine the proportion of equational separations at a locus after "n” chiasmata: En=2/3[1 - (-1/2)n] (Table 1 and page 57) ...
DNA Replication - Gadjah Mada University
... Only some of the genes in a cell are active at any given time, and activity also varies by tissue type and developmental stage. Regulation of gene expression is not completely understood, but it has been shown to involve an array of controlling signals. a. Jacob and Monod (1961) proposed the ope ...
... Only some of the genes in a cell are active at any given time, and activity also varies by tissue type and developmental stage. Regulation of gene expression is not completely understood, but it has been shown to involve an array of controlling signals. a. Jacob and Monod (1961) proposed the ope ...
Exam 2
... Chemical synthesis can produce complex mixtures of small DNA molecules that all are the same length but which differ in base sequence. ...
... Chemical synthesis can produce complex mixtures of small DNA molecules that all are the same length but which differ in base sequence. ...
Section 13.2 Summary – pages 341
... Restriction enzymes cleave (cut) DNA • To isolate a DNA fragment, small pieces of DNA must be cut from a chromosome. • 6. Restriction enzymes are bacterial proteins that have the ability to cut both strands of the DNA molecule at a specific nucleotide sequence. ...
... Restriction enzymes cleave (cut) DNA • To isolate a DNA fragment, small pieces of DNA must be cut from a chromosome. • 6. Restriction enzymes are bacterial proteins that have the ability to cut both strands of the DNA molecule at a specific nucleotide sequence. ...
Biology 520 Second Sem exam
... Graded on a 200 point scale (20 free points) Study Center will be open Mon-Thurs during your free periods and after school. There are handouts available on general exam study/test taking strategies as well. In addition to terms be sure to look at drawings and all Multiple Choice and Review Questions ...
... Graded on a 200 point scale (20 free points) Study Center will be open Mon-Thurs during your free periods and after school. There are handouts available on general exam study/test taking strategies as well. In addition to terms be sure to look at drawings and all Multiple Choice and Review Questions ...
GENETICS The Future of Medicine
... Human Genome Project, the international quest to understand the genomes of humans and other organisms, will shed light on a wide range of basic questions, like how many genes we have, how cells work, how living things evolved, how single cells develop into complex creatures, and what exactly happens ...
... Human Genome Project, the international quest to understand the genomes of humans and other organisms, will shed light on a wide range of basic questions, like how many genes we have, how cells work, how living things evolved, how single cells develop into complex creatures, and what exactly happens ...
TOPICS FOR EXAMINATION II – Biology 1406
... Know the definition of chemoheterotrophs, photoheterotrophs, chemoautotrophs, photoautotrophs. Which of these are involved in human disease? Know the terms for the various preferences for oxygen, CO2, salt (osmotic pressure), temperature, and pH. Which of these terms pertain most to microorganisms f ...
... Know the definition of chemoheterotrophs, photoheterotrophs, chemoautotrophs, photoautotrophs. Which of these are involved in human disease? Know the terms for the various preferences for oxygen, CO2, salt (osmotic pressure), temperature, and pH. Which of these terms pertain most to microorganisms f ...
B left E
... 17. What are the common functions of DNA polymerase III and Klenow fragment? A. 3’ to 5’ polymerase & 3’ to 5’ exonuclease B. 5’ to 3’ polymerase & 5’ to 3’ exonuclease C. 5’ to 3’ polymerase & 3’ to 5’ exonuclease D. 5’ to 3’ polymerase, 3’ to 5’ exonuclease, & 5’ to 3’ exonuclease E. 5’ to 3’ poly ...
... 17. What are the common functions of DNA polymerase III and Klenow fragment? A. 3’ to 5’ polymerase & 3’ to 5’ exonuclease B. 5’ to 3’ polymerase & 5’ to 3’ exonuclease C. 5’ to 3’ polymerase & 3’ to 5’ exonuclease D. 5’ to 3’ polymerase, 3’ to 5’ exonuclease, & 5’ to 3’ exonuclease E. 5’ to 3’ poly ...
nitrogen bases
... What is the Law of Independent Assortment? • Alleles of different genes separate independently of one another during gamete formation • What does that mean? • The allele a parent gives for one trait does not affect what he/she gives for another trait so you can have many different combinations of t ...
... What is the Law of Independent Assortment? • Alleles of different genes separate independently of one another during gamete formation • What does that mean? • The allele a parent gives for one trait does not affect what he/she gives for another trait so you can have many different combinations of t ...
Conjugation
... have been transferred and the order of gene transfer. The longer the cells conjugate, the more of the donor bacterial chromosome moves into the recipient Fcell. The pilus is broken at various times by placing the bacteria in a blender The F- cell does not become F+ or Hfr because the F factor does n ...
... have been transferred and the order of gene transfer. The longer the cells conjugate, the more of the donor bacterial chromosome moves into the recipient Fcell. The pilus is broken at various times by placing the bacteria in a blender The F- cell does not become F+ or Hfr because the F factor does n ...
Cre-Lox recombination
In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.