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Chapter 15: PowerPoint
Chapter 15: PowerPoint

... one base for another • transitions or transversions • also called missense mutations – nonsense mutations – create stop codon – frameshift mutations – caused by insertion or deletion of a single base ...


... assembly 7 of the Neurospora genome (Galagan et al., 2003), this cosmid maps to linkage group IV on contig 43, while un-4 and lys-5 are on linkage group VI. Based on the location of lys-5 (NCU05526) on contig 22, we chose several cosmids from contig 22 and found that two did restore the ability to g ...
BIOL 1107 - Chapter 15
BIOL 1107 - Chapter 15

ch 15 - Quia
ch 15 - Quia

... one base for another • transitions or transversions • also called missense mutations – nonsense mutations – create stop codon – frameshift mutations – caused by insertion or deletion of a single base ...
The Nature of Genes The Nature of Genes The Nature of Genes The
The Nature of Genes The Nature of Genes The Nature of Genes The

... ribosomal RNA (rRNA) is a structural component of the ribosome transfer RNA (tRNA) carries amino acids to the ribosome for translation ...
details
details

... obvious questions to ask is, does it contain a gene? Because genomes of organisms consist of many non-coding regions, it's not clear that a random piece of DNA will always have a gene. And if there is a gene, where does it begin and end? A simple strategy for finding genes is to look for open readin ...
Chp 18 Viruses and Bacteria
Chp 18 Viruses and Bacteria

... DNA is chemically altered, so it is not destroyed by the cell's own restriction enzymes. Restriction enzymes = Naturally occurring bacterial enzymes that protect bacteria against intruding DNA from other organisms. The enzymes also catalyze restriction, the process of cutting foreign DNA into small ...
Ratio of DNA Concentrations
Ratio of DNA Concentrations

... humans and almost all other organisms. Most DNA is located in the nucleus of membrane-bound cells, which constitutes for nearly ever cell in a human’s body. To release DNA, the cell membranes must be lysed. The sugar and phosphate components located on the backbone of DNA are soluble in water. The p ...
7. APPLICATIONS - UTH e
7. APPLICATIONS - UTH e

... Microsatellite DNA Methodology Microsatellites (sometimes referred to as a variable number of tandem repeats or VNTRs) are short segments of DNA that have a repeated sequence such as CACACACA, and they tend to occur in non-coding DNA. In some microsatellites, the repeated unit (e.g. CA) may occu ...
Plant Genome Mapping: Strategies And Applications
Plant Genome Mapping: Strategies And Applications

6.1 Mutation
6.1 Mutation

... Your DNA has instructions to make the proteins!!!! Changes in your DNA are copied into your RNA. That faulty RNA is used to pick the amino acids in your protein. What could possibly happen if you have the wrong amino acids???? ...
Answer Key
Answer Key

... In pea plants, spherical seed shape (S) is dominant to dented seed shape (s) and yellow seeds (Y) are dominant to green seeds (y). If two pea plants, each heterozygous for both traits, are crossed, what is the probability of the offspring having dented, yellow seeds? Show workings. ...
Exam Review 4B - Iowa State University
Exam Review 4B - Iowa State University

... 13. Which of the following is located furthest upstream? a. Enhancers b. Core Promoter c. Regulatory Promoter d. Structural Genes 14. Which of the following is not a way that repressors act? a. Compete with activators b. Bind to the activator c. Bind the silencer d. Allow for assembly of basal tx ap ...
The MYB and BHLH Transcription Factor Families
The MYB and BHLH Transcription Factor Families

... Chiu ...
Prostate cancer cell lines case study on cell cycle map
Prostate cancer cell lines case study on cell cycle map

... Two  interpreta1ons  are  possible  for  the  LNCAP  cells:   -­‐  most  cells  are  expressing  genes  of  the  G1/S  checkpoint.  The  LNCAP  cells  could  try  to  overpass  the   checkpoint  with  less  success  than  DU145  cells. ...
Leukaemia Section t(X;11)(q21;q23) Atlas of Genetics and Cytogenetics in Oncology and Haematology
Leukaemia Section t(X;11)(q21;q23) Atlas of Genetics and Cytogenetics in Oncology and Haematology

... Dual-color interphase FISH analysis of the 11q23 and Xq21 breakpoints with BAC clones. (A) FISH analysis with 11q23 specific BACs: RP11-468P24 (red signals) and RP11-206G12 (green signals). Three red signals indicate translocation within the genomic region represented by RP11-468P24. (B) FISH analys ...
Gene7-04
Gene7-04

... to evolve together as though constituting a single locus. Crossover fixation refers to a possible consequence of unequal crossing-over that allows a mutation in one member of a tandem cluster to spread through the whole cluster (or to be eliminated). Gene conversion is the alteration of one strand o ...
Protein synthesis ppt
Protein synthesis ppt

...  HIV and certain tumor viruses (called retroviruses) have RNA as their infectious information molecule; they convert it to a DNA copy inside the host cell and then use it to make more RNA. ...
Chapter 20 DNA Technology and Genomics
Chapter 20 DNA Technology and Genomics

... Gel electrophoresis separates macromolecules on the basis of their rate of movement through a gel in an electric field. How far a DNA molecule travels while the current is on is inversely proportional to its length. A mixture of DNA molecules, usually fragments produced by restriction enzyme digesti ...
NedGeneticsCompRecomb12 51 KB
NedGeneticsCompRecomb12 51 KB

... Complementation tests allow you to see if two mutations affect the same gene or affect two different genes in a pathway. Pathway Models: -The three models of epistasis we discussed all involve pathways. -Interactions we looked at in plants control pigment formation. What is the advantage to controll ...
Genetics Unit Study Guide – Teacher Version
Genetics Unit Study Guide – Teacher Version

... A family has eight children. Six children have second toes that are longer than the big toe. Two children have second toes that are shorter than the big toe. What are the most likely genotypes of the parents? ...
Nature Rev.Genet. 8
Nature Rev.Genet. 8

... Brg1 forms a complex with HDAC and PARP and triggers a shift from a-myosin heavy chain expression to b-myosin heavy chain expression from Hang et al., Nature 466, 62 (2010) ...
DNA
DNA

... Concept 20.1: DNA cloning yields multiple copies of a gene or other DNA segment • To work directly with specific genes, scientists prepare well-defined segments of DNA in identical copies, a process called DNA cloning ...
EXPLORE THE ISSUE BEING INVESTIGATED
EXPLORE THE ISSUE BEING INVESTIGATED

The effect of sodium ion concentration on
The effect of sodium ion concentration on

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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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