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Portfolio 2 - Biology2Nash
Portfolio 2 - Biology2Nash

... Nucleic Acid and Nucleotides DNA is made of long chains of nucleotides. Each nucleotide contains three basic parts: a base, a deoxyribose molecule, and a phosphate group. There are four different bases: adenine, cytosine, guanine, and thymine. Only one base is found in each nucleotide. Follow the di ...
Origin of Life
Origin of Life

... function. However, no method has been conceived, even in a laboratory, to enrich a mixture by even 1%, so there is no chance that nature could produce 100% left-hand ones. o The amino acids must be in an exact sequence for the protein to fold into the proper 3-dimensional shape to function. If even ...
Mutations Worksheet
Mutations Worksheet

... BONUS: You have a DNA sequence that codes for a protein and is 105 nucleotides long. A frameshift mutation occurs at the 85th base - how many amino acids will be correct in this protein? SHOW YOUR WORK. ...
doc BIOL 200 final notes
doc BIOL 200 final notes

... lagging strand shortens at each cell division - Telomerase: protein-RNA complex, serves as template for addition of deoxyribonucleotides to the ends of telomeres; thus telomerase is a reverse transcriptase that carries it own internal RNA template to direct DNA synthesis - Telomerase is active only ...
RECOMBINANT DNA TECHNOLOGY: APPLICATIONS IN THE
RECOMBINANT DNA TECHNOLOGY: APPLICATIONS IN THE

... human genome. The human genome is more or less the same in everybody - the same genes will be in the same order with the same stretches of intergenic DNA between them. But the human genome, as well as those of other organisms, contains polymorphisms, positions where the nucleotide sequence is not th ...
Computational methods for the analysis of bacterial gene regulation
Computational methods for the analysis of bacterial gene regulation

... bacterial family simultaneously in a laboratory setting. Therefore early on in microbial research, representative organisms of phylogenetic groups of bacteria have been selected as model organisms. The bacterial species a model organism represents is context‐sensitive and thus no ...
IFU COL G 18 set 2013
IFU COL G 18 set 2013

Genetics
Genetics

... suited to adaptation to changes in the environment. ...
(Students with questions should see the appropriate Professor)
(Students with questions should see the appropriate Professor)

... Based on the previous statements, which one of the following analysis is RIGHT? (a) Statements 1) , 2) and 5) are right, while statements 3) and 4) are false. (b) Statements 2) and 5) are right, while statements 1), 3) and 4) are false. (c) Only statement 5) is right, all others are false. (d) State ...
DNA Markers: Explanation of Validation and Utilization
DNA Markers: Explanation of Validation and Utilization

... leptin gene and the growth hormone receptor gene and various carcass traits. Effects for the leptin markers on carcass traits could not be validated. The growth hormone receptor gene marker had significant effects on yield grade and ribeye area, but not on marbling, carcass weight, or fat thickness. ...
(Students with questions should see the appropriate Professor)
(Students with questions should see the appropriate Professor)

... Based on the previous statements, which one of the following analysis is RIGHT? (a) Statements 1) , 2) and 5) are right, while statements 3) and 4) are false. (b) Statements 2) and 5) are right, while statements 1), 3) and 4) are false. (c) Only statement 5) is right, all others are false. (d) State ...
Establishment of a screening service for BM and UCMD
Establishment of a screening service for BM and UCMD

... – 2 x PTC mutations → No functional protein • “Classical” BM: – 1 x Missense/in-frame del/splice → Weak dom-neg effect • Glycine missense in TH domain: – Evidence that N-term Glycine changes cause ‘kinking’ of tetramers → dominant neg effect – Only 1 example of hom glycine change • Het del/splice: – ...
Eukaryotic Transcription
Eukaryotic Transcription

... Where is the termination signal located? What unwinds or melts the DNA to allow for transcription What is another name for RNA transcript? ...
Book 1.indb
Book 1.indb

... receptor, membrane or cytosolic proteins including kinases and phosphatases to convey the signal, and key transcription factors capable of switching their states, activating or suppressing transcription of particular genes. DNA repair systems remove damages. Multiple proofreading mechanisms recogniz ...
Bubbling Liver - DNALC::Protocols
Bubbling Liver - DNALC::Protocols

... example, lipases target fats, amylases target starch, and proteases target proteins. Most of the chemical digestion of food occurs in the small intestine by enzymes produced in the pancreas or in the intestine itself. Lactase enzyme is secreted in the lining of the small intestine. Individuals who a ...
magnetic waves enable cell communication.
magnetic waves enable cell communication.

... would not be possible. To prevent this from happening, neutral resonators are required on both sides, which are not encrypted and do not have to transport information. These include the so-called ‘‘introns,’’ which are in far superior numbers in the DNA strand compared with the information-bearing ‘ ...
Topic 3.5 powerpoint
Topic 3.5 powerpoint

... fragments of DNA according to size. PCR can be used to amplify small amounts of DNA. DNA profiling involves comparison of DNA. Genetic modification is carried out by gene transfer between species. Clones are groups of genetically identical organisms, derived from a single original parent cell. Many ...
11.2 Meiosis
11.2 Meiosis

... & crossing over occurs B. Metaphase I 1. Chromosomes moved to equator by spindle fibers. 2. Two chromatids remain together 3. Independent assortment a. Random distribution of homologous chromosomes ...
PCR amplification of the bacterial genes coding for nucleic acid
PCR amplification of the bacterial genes coding for nucleic acid

... Take following stock solutions out of the freezer and slowly thaw the frozen contents before starting with the PCR mixes One forward (fw) and one reverse (rv) primer stock solution (5μM each)  the primer pairs in use anneal with highly conserved regions of the Citrobacter freundii (Cf) 16S-rRNA gen ...
Cell Division - OpenStax CNX
Cell Division - OpenStax CNX

... The DNA double helix is wrapped around histone proteins to form structures called nucleosomes. Nucleosomes and the linker DNA in between them are coiled into a 30-nm ber. During cell division, chromatin is ...
Biology Single Nucleotide Polymorphisms Lab
Biology Single Nucleotide Polymorphisms Lab

... known length into millions of copies of itself. At the end of the PCR process, we should have a tube with millions of exact copies of that gene in solution. If, however, you possess the SNP, the restriction enzyme would have cut this strand of DNA into two shorter fragments. How do we sort this out? ...
gene expression - cloudfront.net
gene expression - cloudfront.net

... strand of mRNA is synthesized according to the nitrogenous base code of DNA. Transcription can be summarized by the following steps: 1. The enzyme, RNA polymerase, binds to an area of one of the DNA molecules in the double helix. During transcription, only one DNA strand serves as a template (a guid ...
Chapter 20 PPT
Chapter 20 PPT

... Concept 20.1: DNA cloning yields multiple copies of a gene or other DNA segment • To work directly with specific genes, scientists prepare well-defined segments of DNA in identical copies, a process called DNA cloning ...
Document
Document

... where is the head? Where is the tail? Fortunately, there are restriction endonucleases which cleave DNA at specific base sequence they recognize, hence named restriction enzymes. An endonuclease is a pair of DNA scissors that catalyzes (hydrolytic) cleavage of a DNA molecule at specific base sequenc ...
Gene Sequencing
Gene Sequencing

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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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