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... TRANSFAC® is a database on eukaryotic cis-acting regulatory DNA elements and trans-acting factors. It covers the whole range from yeast to human. It started 1988 with a printed compilation and was transferred into computerreadable format in 1990. The FACTOR table contains 6133 entries in 50 classes, ...
Managing people in sport organisations: A strategic human resource
Managing people in sport organisations: A strategic human resource

... the recognition sequence if the cytosine in the sequence is methylated. Normal male control DNA with a CGG-repeat number of 22 on his single X chromosome (lane 1) generates a band about 2.8 kb in length corresponding to Eag1-EcoR1 fragments (see Figure 28.1). Normal female control DNA with a CGG-rep ...
Training
Training

12.3 RNA and Protein Synthesis
12.3 RNA and Protein Synthesis

... reached, the ribosome releases it’s polypeptide chain and the mRNA ...
Chapter_17_answers
Chapter_17_answers

... o strand of 80 nucleotides folded into cloverleaf-like shape o 45 different tRNA’s  the base-pairing rules relax a bit on tRNA molecules; this is known as wobble o ex: U in 3rd position of anticodon can pair with A or G o ex: I (inosine) in 3rd position of anticodon can pair with U, C, or A o aha! ...
Activity 19.4, DNA Sequencing
Activity 19.4, DNA Sequencing

... “DNA Sequencing is a laboratory method of determining the nucleotide sequence of a DNA fragment. The most popular method, sometimes called dideoxysequencing, was worked out by Frederick Sanger in 1974, and so is also called Sanger sequencing. The method utilizes DNA polymerase in vitro to perform a ...
(STAN): looking for nucleotidic and peptidic patterns in
(STAN): looking for nucleotidic and peptidic patterns in

... efficiently scanning sequences as large as human chromosomes, it guarantees to find all occurrences of complex patterns and provides three different types of search operations as explained below. STAN patterns can be used to model sequence and/or structural features of nucleotidic and peptidic seque ...
Mutations
Mutations

... multiple copies of the same chromosome OR multiple portions of the same chromosome.  If this occurred in an egg or sperm cell, disorders like Down Syndrome (Trisomy 21) can occur in the offspring. ...
Ch_17 From Gene to Protein
Ch_17 From Gene to Protein

... because… one gene can code for several protein products, some genes code only for RNA, two genes can overlap, and there are many other complications.” RNA gene – Elizabeth Pennisi, Science 2003 polypeptide 1 ...
Biotechnology Lectures (PowerPoints)
Biotechnology Lectures (PowerPoints)

... B. Genetic engineering brings about such change by scientifically altering an organism's genetic code. ...
national unit specification: general information
national unit specification: general information

... The five Outcomes which make up the Unit are described below; Outcome 1 You will be introduced to the concept of the structural features of DNA and how it is replicated inside the cell. Outcome 2 This Outcome focuses on the cell cycle in which you will look at its control. You will also study the be ...
3-4 Student
3-4 Student

... Copyright Pearson Prentice Hall ...
Epigenetic effects of the Krüppel-like Transcription
Epigenetic effects of the Krüppel-like Transcription

... are different versions of the ChIP techniques, this experiment will take deploy the MeDIP-chip version due to its ability to analyze an entire genome and due to its specialization in DNA methylation. MeDIP-chip works by first randomly shearing the DNA (cutting into small fragments) in the nucleus wi ...
Interfacial Behavior of a Hairpin DNA Probe Immobilized on Gold
Interfacial Behavior of a Hairpin DNA Probe Immobilized on Gold

Macromolecules ( Carbohydrates, Lipids, Proteins and Nucleic Acids )
Macromolecules ( Carbohydrates, Lipids, Proteins and Nucleic Acids )

... with your shoulder partner – Share- When I ask raise your hand to share with the class ...
Mutations Notes Sheet
Mutations Notes Sheet

... Check Your Understanding: Name the type of mutation involved in each of the following cases. 1. Guanine has been “erased” from the nucleotide sequence. ...
The sternum is to the arm. Lateral Medial Superior Inferior In what
The sternum is to the arm. Lateral Medial Superior Inferior In what

... a. They are mutations that everyone has in our DNA. b. They cause restriction enzymes to cut DNA in different locations. c. They are used to identify individuals based on their gel electrophoresis - DNA fingerprint. d. All of the above. ...
Chapter 3 Protein Synthesis Life Science RNA – Ribonucleic Acid
Chapter 3 Protein Synthesis Life Science RNA – Ribonucleic Acid

... Each codon specifies a particular amino acid There are 20 different amino acids There are 64 different combinations of A, U, G, and C that a codon could have ( 4x4x4) There are three “stop” codons acting as a “period” in a sentence The “sentence” is that strip of mRNA produced by the section of expo ...
A Frameshift Mutation Leading to Type 1
A Frameshift Mutation Leading to Type 1

... site polymorphism within the ATIII gene showed that both copies of the gene in the affected individuals are present and grossly intact. Such a gross mapping strategy will not detect small rearrangements within an allele, or disturbance of the 3'-end of the gene, but it can a t least indicate the pre ...
Sequencing the Human Genome
Sequencing the Human Genome

... prepared and immobilized. This involves randomly fragmenting the DNA of interest, anchoring the DNA fragment to a solid surface, and eliminating one of the strands. These steps produce detectable and distinct areas of short, identical DNA molecules. These DNA molecules are then allowed to rebuild th ...
Genetic Coding in Ce..
Genetic Coding in Ce..

... •Are made up of 2 subunits, a large one and a smaller one, each subunit contains ribosomal RNA (rRNA) & proteins. •Protein synthesis starts when the two subunits bind to mRNA. •The initiator codon AUG binds to the first anticodon of tRNA, signaling the start of a protein. ...
BB30055: Genes and genomes
BB30055: Genes and genomes

... signal transduction and immune function) However, only 3 cases where a combination of 3 domain types shared by human & yeast proteins. e.g carbomyl-phosphate synthase (involved in the first 3 steps of de novo pyrimidine biosynthesis) has 7 domain types, which occurs once in human and yeast but twice ...
Standard 5 Lesson Plans
Standard 5 Lesson Plans

... The information in DNA is stored as a code made up of four chemical bases: adenine (A), guanine (G), cytosine (C), and thymine (T). Human DNA consists of about 3 billion bases, and more than 99 percent of those bases are the same in all people. The order, or sequence, of these bases determines the i ...
Genetic dissection of Helicobacter pylori AddAB role in homologous
Genetic dissection of Helicobacter pylori AddAB role in homologous

... single addB mutant is extremely sensitive to IR. Inactivating both genes, addA and addB, resulted in the same sensitivity as that of the single mutants (Fig. 2b). These results confirm that AddA and AddB act together in the repair of IR-induced DNA damage. Inactivation of the AddAB complex made the ...
1. True or false? Genes that are located sufficiently close together in
1. True or false? Genes that are located sufficiently close together in

... B.  the frequency of double crossovers is 1/4 of the number expected if there were no interference. 9  C.  there were four times as many single crossovers as double crossovers.   D.  there were four times as many single crossovers in one region as there were in an adjacent region.   E.  there were f ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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