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Nucleic acids and protein synthesis
Nucleic acids and protein synthesis

... ficient quantity to determine their composition. ...
The genetic code is a degenerate, non-overlapping set of
The genetic code is a degenerate, non-overlapping set of

... called the nucleoid region. Bacterial and Archaeal chromosomes are covalently-closed circles that are not as extensively compacted as eukaryotic chromosomes, but are compacted nonetheless as the diameter of a typical prokaryotic chromosome is larger than the diameter of a typical prokaryotic cell. A ...
LECTURE 5: LINKAGE AND GENETIC MAPPING Reading for this
LECTURE 5: LINKAGE AND GENETIC MAPPING Reading for this

... chromosomes during meiosis. Both groups followed chromosomes that were physically marked with cytologically visible abnormalities, so that a maternally-derived homolog could be easily distinguished from the paternal one. The marked chromosomes also carried mutations in order to monitor which progeny ...
a code for traits: dna structure and function
a code for traits: dna structure and function

... Just as an architect uses a blueprint to construct a building, an organism’s DNA is a blueprint for its traits. The blueprints for the White House are different from the blueprints for the Washington Monument, making these two buildings different on a structural level. It makes sense, therefore, tha ...
Bio nformatics - City University of New York
Bio nformatics - City University of New York

... • Ribose instead of deoxyribose. • RNA does not contain Thymine T, instead Uracil U is present (which also binds with A). • RNA does not form a double helix. Saad Mneimneh ...
Standard B-5 - Wando High School
Standard B-5 - Wando High School

... ○ One pair of chromosomes in an organism determines the sex (male, female) of the organism; these are known as sex chromosomes. All other chromosomes are known as autosomal chromosomes, or autosomes. ○ Cells (except for sex cells) contain one pair of each type of chromosome.  Each pair consists of ...
molecular biology
molecular biology

... nucleic acid either from its 5’-end (the 5’ 3’ exonuclease) or from its 3’-end (the 3’ 5’ exonuclease). It will therefore remove one nucleotide at a time and at each cleavage, the length of the nucleic acid will be reduced by one nucleotide. Upon complete digestion the nucleic acid will be converted ...
File - Year 11 Science
File - Year 11 Science

...  double helix  (contains) bases  A, T, C, G  adenine / A paired with thymine / T  guanine / G paired with cytosine / C  hydrogen / H bonds joining basesContributions from Scientists:  X-ray (crystallography) being used  to show helical structure  to show diameter of molecule  how ...
Genetic Engineering
Genetic Engineering

... host cell allowed to multiply required DNA fragment cut out of appropriate chromosome duplicate plasmids formed which express ‘foreign’ gene plasmid extracted from bacterium and opened up altered plasmid inserted into bacterial host cell DNA fragment sealed into plasmid ...
Transplantation Immunology pg. 1 Laura Rayne Today I`m going to
Transplantation Immunology pg. 1 Laura Rayne Today I`m going to

... Replication of chromosomal DNA is initiated at a very specific site. Some organisms can start at different sites, but bacteria only start at one site, called the oriC. It requires many enzymes. Large amounts of protein complexes are required to divide. These proteins must recognize the oriC, separat ...
Highlight of mutation GPS® technique
Highlight of mutation GPS® technique

... A deletion is a mutation caused by loss of a DNA sequence. An insertion is a mutations caused by adding a piece of DNA into genome, which can occur naturally, or can be artificially created for research purposes in the lab mediated by virus, plasmid or transposons. Exogenous DNA insertion mutations ...
Protein Synthesis
Protein Synthesis

... tRNA's read the next codon. In the example on the left the next tRNA to read the mRNA is tyrosine. When the correct match with the anticodons of a tRNA has been found, the tyrosine forms a peptide bond with the growing peptide chain . The proline is now hydrolyzed from the tRNA. The proline tRNA now ...
bio eoc study guide
bio eoc study guide

... apart. The new protein is released. 34. Genetic code - 1 codon = 3 bases (codes for 1 amino acid). Total 64 codons. Codons - Instructions written as a series of 3 nucleotide sequences on the mRNA. Stop codons - UAA, UAG, UGA. Start codon - AUG.(Methionine) ...
Divergence of Sulfur-Flower Buckwheat using DNA Analyses
Divergence of Sulfur-Flower Buckwheat using DNA Analyses

... Eriogonum  umbellatum  from  several  distinct  populations  residing  in  both  Colorado   and  Oregon.  Tissue  samples  were  collected  from  several  individuals  from  each   population.  The  five  varieties  of  interest  include  E. ...
Powerpoint document
Powerpoint document

... • Other functions of nucleotides: • serving as energy stores (mainly ATP) • controlling numerous enzymatic reactions through allosteric effects on enzyme activity • mediators of numerous important cellular processes such as second messengers in signal transduction events ...
Mitochondria damage checkpoint in apoptosis and genome stability
Mitochondria damage checkpoint in apoptosis and genome stability

... 7. A mitochondria damage checkpoint (mitocheckpoint) Checkpoint was defined by Hartwell and Weinert [37] as control mechanism that ensures the proper order of cellular events by arresting or delaying progression through the cell cycle in response to DNA damage [25]. Based on our comparative gene expr ...
Mutation, Repair, and Recombination
Mutation, Repair, and Recombination

... Why does this ability make biological sense? Answer: DNA in E. coli is methylated. To distinguish the old template strand from the newly synthesized strand, the mismatch repair mechanism takes advantage of a delay in the methylation of the new strand. This makes sense as replication errors produce m ...
Genome Editing Using Cas9 Nickases
Genome Editing Using Cas9 Nickases

... Nucleases with off-target DSB activity could induce undesirable mutations with potentially deleterious effects, an unacceptable outcome in most clinical settings. The remarkable ease of targeting Cas9 has enabled extensive off-target binding and mutagenesis studies employing deep sequencing (Fu et a ...
The Genetic Code
The Genetic Code

... (b, 5 pts) Now you ligate the DNA you produced in part (a) to the sequence below, which you have also cut with the same restriction enzyme. Draw the shortest DNA product that could form from ligating a piece of DNA from part (a) to a piece of DNA from part (b). Make sure to draw the nucleotide seque ...
(STC) approach with a non selective AFLP fingerprinting
(STC) approach with a non selective AFLP fingerprinting

... MegaBlast analysis against assembled tomato contigs. Position and direction of overlap were verified, and candidate BAC clones are preselected setting a threshold expect value to 0.0 or an identity score treshold of 0.99. When meeting constraints, corresponding ABI traces are subsequently assembled ...
The Nobel Prize in Physiology or Medicine 2007
The Nobel Prize in Physiology or Medicine 2007

Genetic engineering of human FSH (Gonal
Genetic engineering of human FSH (Gonal

... for its function and interaction with receptor/substrate. A single alteration (mutation) in the amino acids sequence can render the protein inactive. In order for many of the proteins to be able to carry out their functions correctly, they must have a particular 3D structure. For instance, with an e ...
13-2 Manipulating DNA
13-2 Manipulating DNA

... Cutting and Pasting Short sequences of DNA can be assembled using DNA synthesizers. “Synthetic” sequences can be joined to “natural” sequences using enzymes that splice DNA together. ...
13-2 - Lincoln Park High School
13-2 - Lincoln Park High School

... Cutting and Pasting Short sequences of DNA can be assembled using DNA synthesizers. “Synthetic” sequences can be joined to “natural” sequences using enzymes that splice DNA together. ...
13-2 Manipulating DNA
13-2 Manipulating DNA

... Separating DNA In gel electrophoresis, DNA fragments are placed at one end of a porous gel, and an electric voltage is applied to the gel. When the power is turned on, the negativelycharged DNA molecules move toward the positive end of the gel. ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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