The common northern periwinkle, Littorina littorea
... phospholipids (Fig. 1). Several glycolytic enzymes are subject to anoxia-induced phosphorylation that suppresses their activity. These include glycogen phosphorylase (GP), PFK1, 6-phosphofructo-2-kinase (PFK-2), and PK (Storey, 1993). Control over GP regulates the availability of the primary fuel, g ...
... phospholipids (Fig. 1). Several glycolytic enzymes are subject to anoxia-induced phosphorylation that suppresses their activity. These include glycogen phosphorylase (GP), PFK1, 6-phosphofructo-2-kinase (PFK-2), and PK (Storey, 1993). Control over GP regulates the availability of the primary fuel, g ...
The mRNA export machinery requires the novel Sac3p±Thp1p
... Sac3p (Figure 5A, middle panel). We conclude that Sac3p has to be targeted to the nucleoplasmic side of the NPC to perform its function in mRNA export. To determine whether Nup1p or Nup60p are involved in docking Sac3p to the nuclear pores in vivo, we analyzed the location of Sac3p±GFP in nup1D or n ...
... Sac3p (Figure 5A, middle panel). We conclude that Sac3p has to be targeted to the nucleoplasmic side of the NPC to perform its function in mRNA export. To determine whether Nup1p or Nup60p are involved in docking Sac3p to the nuclear pores in vivo, we analyzed the location of Sac3p±GFP in nup1D or n ...
BioPerf: A Benchmark Suite to Evaluate High-Performance
... are molecules that accomplish most of the functions of a living cell, determining its shape and structure. A protein is a linear sequence of molecules called amino acids. Twenty different amino acids are commonly found in proteins. Similar to DNA, proteins are conveniently represented as a string of ...
... are molecules that accomplish most of the functions of a living cell, determining its shape and structure. A protein is a linear sequence of molecules called amino acids. Twenty different amino acids are commonly found in proteins. Similar to DNA, proteins are conveniently represented as a string of ...
Characterization of a Novel Peripheral Nervous System Myelin
... ledto the hypothesis that PO may be responsible for the adhesion between the extracellular surfaces of the myelin plasma membrane. Recent studies on cultured cells clearly demonstrate the capacity of PO to undergo homophilic interactions (Filbin et al ., 1990; Schneider-Schaulies et al ., 1990; D'Ur ...
... ledto the hypothesis that PO may be responsible for the adhesion between the extracellular surfaces of the myelin plasma membrane. Recent studies on cultured cells clearly demonstrate the capacity of PO to undergo homophilic interactions (Filbin et al ., 1990; Schneider-Schaulies et al ., 1990; D'Ur ...
Folding minimal sequences: the lower bound for sequence
... proteins Wild-type SH3 domains appear to have a higher complexity, a narrower standard deviation, and a narrower complexity domain compared to the folded proteins in NRL-3D, by both the alphabet and entropy measures (Table 2 and Fig. 3). The signi¢cance of these ¢ndings is unclear, but it is perhaps ...
... proteins Wild-type SH3 domains appear to have a higher complexity, a narrower standard deviation, and a narrower complexity domain compared to the folded proteins in NRL-3D, by both the alphabet and entropy measures (Table 2 and Fig. 3). The signi¢cance of these ¢ndings is unclear, but it is perhaps ...
Identification of genes from flat oyster Ostrea
... The parasite Bonamia ostreae was first reported in France in 1979 infecting the European flat oyster, Ostrea edulis [1, 2]. Despite 30 years of research on this protozoan pathogen, host parasite interactions are poorly known and few sequence data is available for O. edulis in public databases. A rec ...
... The parasite Bonamia ostreae was first reported in France in 1979 infecting the European flat oyster, Ostrea edulis [1, 2]. Despite 30 years of research on this protozoan pathogen, host parasite interactions are poorly known and few sequence data is available for O. edulis in public databases. A rec ...
A MODEL FOR THE PROTEOLYTIC REGULATION OF
... acetate (Young et al., 1995). It is known that very low levels of LpxC are detrimental to the cell viability (Schakermann et al., 2013); however, very high levels of this enzyme also appear to be toxic to the organism presumably due to a build up of lipid A (Sorensen et al., 1996). Therefore, regula ...
... acetate (Young et al., 1995). It is known that very low levels of LpxC are detrimental to the cell viability (Schakermann et al., 2013); however, very high levels of this enzyme also appear to be toxic to the organism presumably due to a build up of lipid A (Sorensen et al., 1996). Therefore, regula ...
Chpt14_Translation.doc
... brief time (short relative to the time required to complete synthesis). 2. Completed polypeptides were collected, digested with trypsin, and the amount of radioactivity in tryptic fragments was determined. 3. Tryptic fragments from the C-terminal end of the polypeptide had radioactivity at the earli ...
... brief time (short relative to the time required to complete synthesis). 2. Completed polypeptides were collected, digested with trypsin, and the amount of radioactivity in tryptic fragments was determined. 3. Tryptic fragments from the C-terminal end of the polypeptide had radioactivity at the earli ...
letters Solution structure of the DNA-binding domain of MafG
... The Maf family of transcription factors are basic regionleucine zipper (bZIP) proteins, characterized by the presence of a specific amino acid sequence, the Maf extended homology ...
... The Maf family of transcription factors are basic regionleucine zipper (bZIP) proteins, characterized by the presence of a specific amino acid sequence, the Maf extended homology ...
Polyclonal Antibodies to Lamins - Edinburgh Research and Innovation
... proteins ‐ a region which has multiple binding partners and is, therefore, often masked. These antibodies have been made to the chromatin binding region of the Lamin proteins – a region which is the least masked at the nuclear envelope. Consequently, they are highly specific and give clear stron ...
... proteins ‐ a region which has multiple binding partners and is, therefore, often masked. These antibodies have been made to the chromatin binding region of the Lamin proteins – a region which is the least masked at the nuclear envelope. Consequently, they are highly specific and give clear stron ...
TONE UP. GET LEAN. BE STRONG.
... Adapted from: Layman DK, USDA Food Composition Tables, J Nutr 133:261S-267S, 2003. Whey protein isolate offers more branched-chain amino acids and leucine than other typical protein ingredients. ...
... Adapted from: Layman DK, USDA Food Composition Tables, J Nutr 133:261S-267S, 2003. Whey protein isolate offers more branched-chain amino acids and leucine than other typical protein ingredients. ...
A Comparison of Suffix Tree based Indexing and Search
... represent 3-D substructures as compared to sequence of strings in the other two approaches.In this section we are going to see how the features are extracted and data is prepared for construction of suffix trees. From the chain of carbon atoms PSIST uses a sliding window to extract local feature vec ...
... represent 3-D substructures as compared to sequence of strings in the other two approaches.In this section we are going to see how the features are extracted and data is prepared for construction of suffix trees. From the chain of carbon atoms PSIST uses a sliding window to extract local feature vec ...
pIVEX - ISBG
... end of chapter 4.1). or • Prepare a cloning fragment by limited digestion if desired restriction site is present in the gene (refer to the literature given at the end of chapter 4.1). The pIVEX vectors are especially optimized for use in RTS cell-free protein expression systems. However, any DNA ins ...
... end of chapter 4.1). or • Prepare a cloning fragment by limited digestion if desired restriction site is present in the gene (refer to the literature given at the end of chapter 4.1). The pIVEX vectors are especially optimized for use in RTS cell-free protein expression systems. However, any DNA ins ...
D-Isonucleotide (isoNA) incorporation around cleavage site of
... first recognized by the RLC complex, which contains Dicer and the RNA-binding protein TRBP, to form an immature RISC complex.7 Then, the passenger strand is nicked between positions 9 and 10, counted from the 5′-terminal, by the Argonaute 2 (Ago2) protein in the same way as target mRNA is cleaved,8, ...
... first recognized by the RLC complex, which contains Dicer and the RNA-binding protein TRBP, to form an immature RISC complex.7 Then, the passenger strand is nicked between positions 9 and 10, counted from the 5′-terminal, by the Argonaute 2 (Ago2) protein in the same way as target mRNA is cleaved,8, ...
search_2009
... How to filter results from large sequences and queries? 1. Some sequences contain large regions of ALU repeats. In this case you can select the "Human Repeat" filtering option on the main BLAST search page. This will mask repeat regions which generate a large number of biologically uninteresting hi ...
... How to filter results from large sequences and queries? 1. Some sequences contain large regions of ALU repeats. In this case you can select the "Human Repeat" filtering option on the main BLAST search page. This will mask repeat regions which generate a large number of biologically uninteresting hi ...
Sequence-Specific Resonance Assignments of the `H
... as the simian immunodeficiency virus (SIV), the bovine immunodeficiency virus (BIV), the feline immunodeficiency virus (FIV), the sheep visna virus (VILV), the caprine arthritis encephalitisvirus (CAEV), and the ovine lentivirus (OMVV). Retroviruses use a complex machinery of RNA-binding proteins to ...
... as the simian immunodeficiency virus (SIV), the bovine immunodeficiency virus (BIV), the feline immunodeficiency virus (FIV), the sheep visna virus (VILV), the caprine arthritis encephalitisvirus (CAEV), and the ovine lentivirus (OMVV). Retroviruses use a complex machinery of RNA-binding proteins to ...
PROTEIN EXTRACTION AND PURIFICATION
... 2. precipitation by increasing the ionic strength (salting out) 1) When high concentrations of salt are present, proteins tend to aggregate and precipitate out of solution. This technique is referred to as "salting out". Factors such as pH, temperature and protein purity play important roles in dete ...
... 2. precipitation by increasing the ionic strength (salting out) 1) When high concentrations of salt are present, proteins tend to aggregate and precipitate out of solution. This technique is referred to as "salting out". Factors such as pH, temperature and protein purity play important roles in dete ...
Ribosome Profiling Provides Evidence that Large Please share
... Ribosome profiling can be used to identify mRNAs associated with 80S ribosomes, distinguish ribosome-free 3′-untranslated regions (3′-UTRs) on these messages, and assess the quantitative dynamics of translation within translated regions (Ingolia et al., 2009; Ingolia et al., 2011). Global identifica ...
... Ribosome profiling can be used to identify mRNAs associated with 80S ribosomes, distinguish ribosome-free 3′-untranslated regions (3′-UTRs) on these messages, and assess the quantitative dynamics of translation within translated regions (Ingolia et al., 2009; Ingolia et al., 2011). Global identifica ...
search1
... How to generate results from large sequences and queries? 1. Some sequences contain large regions of ALU repeats. In this case you can select the "Human Repeat" filtering option on the main BLAST search page. This will mask repeat regions which generate a large number of biologically uninteresting ...
... How to generate results from large sequences and queries? 1. Some sequences contain large regions of ALU repeats. In this case you can select the "Human Repeat" filtering option on the main BLAST search page. This will mask repeat regions which generate a large number of biologically uninteresting ...
Biological Molecules
... One amino acid change in a protein can affect its structure and therefore the function of the protein. ...
... One amino acid change in a protein can affect its structure and therefore the function of the protein. ...
Determination of the Binding Site-Size of the Protein
... crude cell extracts, it is thought that cell extracts contain many other DNA-binding proteins (and their regulatory proteins), and some small molecules (such as ATP and DNA metabolites), that are also able to interact with the DNA and the target protein; these molecules may cause some unpredictable ...
... crude cell extracts, it is thought that cell extracts contain many other DNA-binding proteins (and their regulatory proteins), and some small molecules (such as ATP and DNA metabolites), that are also able to interact with the DNA and the target protein; these molecules may cause some unpredictable ...
lecture08_11
... Different polypeptide chains run alongside each other and are linked together by hydrogen bonds. ...
... Different polypeptide chains run alongside each other and are linked together by hydrogen bonds. ...
Corn Bt 176 - Biotechnology Philippines
... The glufosinate ammonium inhibits the glutamine synthetase in plants, resulting in an accumulation of ammonia in plant tissues which leads to its death. The expression of the PAT protein protects the plant from the herbicide glufosinate ammonium. Safety of the Expressed Proteins Toxicity studies con ...
... The glufosinate ammonium inhibits the glutamine synthetase in plants, resulting in an accumulation of ammonia in plant tissues which leads to its death. The expression of the PAT protein protects the plant from the herbicide glufosinate ammonium. Safety of the Expressed Proteins Toxicity studies con ...
Molecular and General Genetics
... from 94% to 99%) between these genes, and the sequences were equally close to the thyA sequence from B. subtilis 168 and the thyP3 gene from phage /3T. Slight, but signi®cant dierences between the sequences derived from B. subtilis 168, B. amyloliquefaciens S18 and B. atrophaeus S223 on the one han ...
... from 94% to 99%) between these genes, and the sequences were equally close to the thyA sequence from B. subtilis 168 and the thyP3 gene from phage /3T. Slight, but signi®cant dierences between the sequences derived from B. subtilis 168, B. amyloliquefaciens S18 and B. atrophaeus S223 on the one han ...
Supplemental Text
... In the equation for sp in (2), η is the fraction of ribosomes that translate ribosomal protein and kp = kp0 fres is the rate of protein synthesis per ribosome. The function fres (a/V ) describes the increase of the translation rate kp as a function of the intracellular resource concentration a/V . F ...
... In the equation for sp in (2), η is the fraction of ribosomes that translate ribosomal protein and kp = kp0 fres is the rate of protein synthesis per ribosome. The function fres (a/V ) describes the increase of the translation rate kp as a function of the intracellular resource concentration a/V . F ...
Gene expression
Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses - to generate the macromolecular machinery for life.Several steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-translational modification of a protein. Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism.In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, i.e. observable trait. The genetic code stored in DNA is ""interpreted"" by gene expression, and the properties of the expression give rise to the organism's phenotype. Such phenotypes are often expressed by the synthesis of proteins that control the organism's shape, or that act as enzymes catalysing specific metabolic pathways characterising the organism.