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Antisense-mediated FLC transcriptional repression requires the P
... (P-TEFb) (26–29). P-TEFb regulates transcription elongation and integrates mRNA synthesis with histone modification, premRNA processing, and mRNA export (30). Arabidopsis CDKC;2 has previously been shown to be important for flowering time control and plant virus infection (26) and to colocalize with ...
... (P-TEFb) (26–29). P-TEFb regulates transcription elongation and integrates mRNA synthesis with histone modification, premRNA processing, and mRNA export (30). Arabidopsis CDKC;2 has previously been shown to be important for flowering time control and plant virus infection (26) and to colocalize with ...
The N-end rule pathway as a nitric oxide sensor controlling the
... double-mutant [UBR1-/-UBR2-/-] EF cell line; the latter was produced as described in ref. 10 and the main text (Y. T. Kwon and A.V., unpublished data). The UBR1/2dnR2 cells and the parental [UBR1-/-UBR2-/-] cell line were maintained in DMEM plus 10% FBS (Gibco). Other cell lines used were NIH-3T3 (r ...
... double-mutant [UBR1-/-UBR2-/-] EF cell line; the latter was produced as described in ref. 10 and the main text (Y. T. Kwon and A.V., unpublished data). The UBR1/2dnR2 cells and the parental [UBR1-/-UBR2-/-] cell line were maintained in DMEM plus 10% FBS (Gibco). Other cell lines used were NIH-3T3 (r ...
DNA and RNA Purification Selection Guide
... Rapidly isolate transfection-quality plasmid DNA. Vacuum protocol greatly reduces the time spent on purification compared to silica resin or other membrane-column methods. Unique Endotoxin Removal Wash removes substantial amounts of protein, RNA and endotoxin contaminants from purified plasmid DNA t ...
... Rapidly isolate transfection-quality plasmid DNA. Vacuum protocol greatly reduces the time spent on purification compared to silica resin or other membrane-column methods. Unique Endotoxin Removal Wash removes substantial amounts of protein, RNA and endotoxin contaminants from purified plasmid DNA t ...
Identification and Developmental Expression of a Novel Low
... is detectable within the cement gland, within many neuronal cell bodies and axon tracts within the developing nervous system, and within all cellular layers of the developing retina. The availability of these two distinct neuron-specific intermediate filament proteins, with different temporal and sp ...
... is detectable within the cement gland, within many neuronal cell bodies and axon tracts within the developing nervous system, and within all cellular layers of the developing retina. The availability of these two distinct neuron-specific intermediate filament proteins, with different temporal and sp ...
50695_1 - Griffith Research Online
... A protein is made up of a collection of amino acids, which are molecules that have both carboxyl and amino groups. An amino acid contains a carbon atom (Cα), and has four different connections, these include an amino group, carboxyl group, a hydrogen atom, and a side chain (this differs depending on ...
... A protein is made up of a collection of amino acids, which are molecules that have both carboxyl and amino groups. An amino acid contains a carbon atom (Cα), and has four different connections, these include an amino group, carboxyl group, a hydrogen atom, and a side chain (this differs depending on ...
PowerPoint 演示文稿 - Xiamen University
... Figure 7.5 Effects of regulatory mutations in the lac operon in merodiploids. Jacob and Monod and others created merodiploid E.coli strains as described in panels (a)-(b) and tested them for lac products in the presence and absence of lactose. (a) This merodiloid has one wild-type operon (top) and ...
... Figure 7.5 Effects of regulatory mutations in the lac operon in merodiploids. Jacob and Monod and others created merodiploid E.coli strains as described in panels (a)-(b) and tested them for lac products in the presence and absence of lactose. (a) This merodiloid has one wild-type operon (top) and ...
Use of Gene Replacement Transformation to Elucidate
... in Saccharomyces cerevisiae shares many similarities in terms of gene control with the qa gene cluster and is also subjectto carboncatabolite repression (JOHNSTON 1987). Carbon repressionof the GAL genes is thought to operate throughseveral mechanisms such as direct action on the various GAL promote ...
... in Saccharomyces cerevisiae shares many similarities in terms of gene control with the qa gene cluster and is also subjectto carboncatabolite repression (JOHNSTON 1987). Carbon repressionof the GAL genes is thought to operate throughseveral mechanisms such as direct action on the various GAL promote ...
The Two Major Membrane Skeletal Proteins (Articulins) of Euglena
... hE membrane skeleton of higher eukaryotes is required for a number of important cell functions including the maintenance of cell shape, the stabilization of the plasma membrane (reviewed in Elgsaeter et al., 1986; Bennett, 1990), generating and maintaining plasma membrane domains (Rodriguez-Boulan a ...
... hE membrane skeleton of higher eukaryotes is required for a number of important cell functions including the maintenance of cell shape, the stabilization of the plasma membrane (reviewed in Elgsaeter et al., 1986; Bennett, 1990), generating and maintaining plasma membrane domains (Rodriguez-Boulan a ...
Document
... … we found that 2917 of the FANTOM proteins are in fact splice isoforms of known transcripts … – Frey et al Science, March 2006 … the number of new proteincoding genes found by us has been revised from 5154 to 2222… – FANTOM/RIKEN Consortium Science, March 2006 Brendan Frey ...
... … we found that 2917 of the FANTOM proteins are in fact splice isoforms of known transcripts … – Frey et al Science, March 2006 … the number of new proteincoding genes found by us has been revised from 5154 to 2222… – FANTOM/RIKEN Consortium Science, March 2006 Brendan Frey ...
Genome-wide association study of superovulatory response traits in
... cortex during the embryonic development (Pilaz and Silver, 2015). The DENN/MADD domain containing 1A (DENND1A) regulates Rab GTPases, which is, among other things, required for GnRH-induced gonadotropin release (Welt et al., 2012). This protein was reported to be highly present in human brain and te ...
... cortex during the embryonic development (Pilaz and Silver, 2015). The DENN/MADD domain containing 1A (DENND1A) regulates Rab GTPases, which is, among other things, required for GnRH-induced gonadotropin release (Welt et al., 2012). This protein was reported to be highly present in human brain and te ...
3040 - Zhang, Hongyu
... their side chains are then packed into three-dimensional structures referred to as the tertiary structures. Proteins are very important and interesting biomolecules to the scientists in and outside the field of life science not only because of their crucial functional roles in life but also because ...
... their side chains are then packed into three-dimensional structures referred to as the tertiary structures. Proteins are very important and interesting biomolecules to the scientists in and outside the field of life science not only because of their crucial functional roles in life but also because ...
Biological ontologies for human functional annotation and
... “An amino acid monomer, e.g. tyrosine, serine, tyr, ser” ...
... “An amino acid monomer, e.g. tyrosine, serine, tyr, ser” ...
Caenorhabditis elegans unc-60 gene encodes
... vertebrate Z lines, and interdigitate with the thick filaments which are centrally stacked in the sarcomere. An electron-dense material analogous to the vertebrate M line is also present. While there are also significant differences, the similarities to vertebrate muscle make C. eleoans an attractiv ...
... vertebrate Z lines, and interdigitate with the thick filaments which are centrally stacked in the sarcomere. An electron-dense material analogous to the vertebrate M line is also present. While there are also significant differences, the similarities to vertebrate muscle make C. eleoans an attractiv ...
propy: a tool to generate various modes of
... 2000). In fact, the aforementioned features can be regarded as different Chou’s PseAAC modes. For example, amino acid, dipeptide, tripeptide or n-mer peptide (n ¼ 4, 5, . . . ) compositions are just different modes of Chou’s PseAAC. Moreover, the higher-level features, such as GO (Gene Ontology) inf ...
... 2000). In fact, the aforementioned features can be regarded as different Chou’s PseAAC modes. For example, amino acid, dipeptide, tripeptide or n-mer peptide (n ¼ 4, 5, . . . ) compositions are just different modes of Chou’s PseAAC. Moreover, the higher-level features, such as GO (Gene Ontology) inf ...
Molecular Biology 101
... There are many components of a PCR reaction. They include the template, sometimes referred to as the target which is the DNA containing your region of interest. You also need primers, sometimes referred to as oligonucleotides or oligos and these are short pieces of single stranded DNA complementary ...
... There are many components of a PCR reaction. They include the template, sometimes referred to as the target which is the DNA containing your region of interest. You also need primers, sometimes referred to as oligonucleotides or oligos and these are short pieces of single stranded DNA complementary ...
Extension and Enrichment
... R. Sickle cell anemia is caused by a single change in the amino acid sequence. Amino acid 6 is Glutamic acid in normal hemoglobin. It is replaced with Valine in sickle cell anemia. Using the information you just learned, explain why sickle cell anemia leads to deformed blood cells. ...
... R. Sickle cell anemia is caused by a single change in the amino acid sequence. Amino acid 6 is Glutamic acid in normal hemoglobin. It is replaced with Valine in sickle cell anemia. Using the information you just learned, explain why sickle cell anemia leads to deformed blood cells. ...
Chapter 6 notes - s3.amazonaws.com
... Genes and Proteins • Proteins and Traits Proteins act as chemical triggers for many of the processes within cells. Proteins help determine traits. ...
... Genes and Proteins • Proteins and Traits Proteins act as chemical triggers for many of the processes within cells. Proteins help determine traits. ...
Nucleic acid crystallography: current progress
... crystal structures released by the Nucleic Acid Database (NDB [7]). Of a total of 805 such structures currently available through the NDB, 140 were released between January of 2002 and June of 2004. Note: if the numbers per category are added together, the resulting numbers will be greater than 140 ...
... crystal structures released by the Nucleic Acid Database (NDB [7]). Of a total of 805 such structures currently available through the NDB, 140 were released between January of 2002 and June of 2004. Note: if the numbers per category are added together, the resulting numbers will be greater than 140 ...
Protein Structure Analysis
... Proteins that have low sequence identities, but whose structural and functional features suggest that a common evolutionary origin is probable are placed together in superfamilies. For example, actin, the ATPase domain of the heat shock protein, and hexakinase together form a superfamily. ...
... Proteins that have low sequence identities, but whose structural and functional features suggest that a common evolutionary origin is probable are placed together in superfamilies. For example, actin, the ATPase domain of the heat shock protein, and hexakinase together form a superfamily. ...
Ammonium transport in Escherichia coli: localization and nucleotide
... structural gene mutation produced by TnlO insertion. This allowed m t A to be localized within a 1.4 kb region which spans the site of the mutation. By application of the Sanger dideoxy method, we sequenced the region containing umtA. The gene contains an open reading frame which encodes a protein w ...
... structural gene mutation produced by TnlO insertion. This allowed m t A to be localized within a 1.4 kb region which spans the site of the mutation. By application of the Sanger dideoxy method, we sequenced the region containing umtA. The gene contains an open reading frame which encodes a protein w ...
- Cure SMA
... Families of SMA Awards New Drug Discovery Funding to Advance a CNS-Delivered Gene Therapy for Spinal Muscular Atrophy. Families of SMA is pleased to announce the award of up to $750,000 for an important new grant to Dr. Brian Kaspar at Nationwide Children’s Hospital. This award will support preclini ...
... Families of SMA Awards New Drug Discovery Funding to Advance a CNS-Delivered Gene Therapy for Spinal Muscular Atrophy. Families of SMA is pleased to announce the award of up to $750,000 for an important new grant to Dr. Brian Kaspar at Nationwide Children’s Hospital. This award will support preclini ...
1. PROTEIN MODIFICATION 1.1 What are posttranslational
... describe, with a picture where necessary, the conditions and products of the following two strategies: 1) error-prone PCR and 2) random oligonucleotide mutagenesis / overlap extension PCR. What is the main difference between the two approaches, in terms of the type of library that is produced? ...
... describe, with a picture where necessary, the conditions and products of the following two strategies: 1) error-prone PCR and 2) random oligonucleotide mutagenesis / overlap extension PCR. What is the main difference between the two approaches, in terms of the type of library that is produced? ...
Hydrophobic-Hydrophilic Forces and their Effects on Protein
... A protein is made up of a collection of amino acids, which are molecules that have both carboxyl and amino groups. An amino acid contains a carbon atom (Cα), and has four different connections, these include an amino group, carboxyl group, a hydrogen atom, and a side chain (this differs depending on ...
... A protein is made up of a collection of amino acids, which are molecules that have both carboxyl and amino groups. An amino acid contains a carbon atom (Cα), and has four different connections, these include an amino group, carboxyl group, a hydrogen atom, and a side chain (this differs depending on ...
History and Function
... ONE-DIMENSIONAL DIFFUSION The abilitiy to diffuse in one dimension can accelerate the formation of a site-specific interaction within a linear biopolymer by up to 103fold. Such facilitated diffusion is used by transcription factors and restriction endonucleases to locate specific sites on double-str ...
... ONE-DIMENSIONAL DIFFUSION The abilitiy to diffuse in one dimension can accelerate the formation of a site-specific interaction within a linear biopolymer by up to 103fold. Such facilitated diffusion is used by transcription factors and restriction endonucleases to locate specific sites on double-str ...
Human complement factor H: expression of an additional truncated
... tor H protein [lo] is a faithful transcript of a 1.8-kb mRNA constitutively expressed in human liver. A 1-kb cDNA (H-46) coding for the C-terminal epitopes of factor H detected a third 1.0-kb mRNA in addition to the 4.4-kb large factor H transcript or Northern blots. All three factor H-derived mRNA ...
... tor H protein [lo] is a faithful transcript of a 1.8-kb mRNA constitutively expressed in human liver. A 1-kb cDNA (H-46) coding for the C-terminal epitopes of factor H detected a third 1.0-kb mRNA in addition to the 4.4-kb large factor H transcript or Northern blots. All three factor H-derived mRNA ...
Gene expression
Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses - to generate the macromolecular machinery for life.Several steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-translational modification of a protein. Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism.In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, i.e. observable trait. The genetic code stored in DNA is ""interpreted"" by gene expression, and the properties of the expression give rise to the organism's phenotype. Such phenotypes are often expressed by the synthesis of proteins that control the organism's shape, or that act as enzymes catalysing specific metabolic pathways characterising the organism.