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Nuclear Magnetic Resonance (NMR) Spectroscopy
... mation on the diversity of RNA conformations that are found in nature. An altogether different approach has been taken to the labelling of DNA for multinuclear NMR studies. For many years, DNA did not appear to require the application of isotope enrichment techniques. Large quantities of DNA have be ...
... mation on the diversity of RNA conformations that are found in nature. An altogether different approach has been taken to the labelling of DNA for multinuclear NMR studies. For many years, DNA did not appear to require the application of isotope enrichment techniques. Large quantities of DNA have be ...
Molecular Cloning and Nucleotide Sequence of the 3
... the first report of the nucleotide sequence of a functional gene of C. utilis as far as we know. The sequence of 2209 bp was an alignment of four restriction fragments determined separately. The sequences of HindIII-CluI, CluI-EcoRI, EcoRI-BglII and BglII-Hind111 fragments could be clearly read from ...
... the first report of the nucleotide sequence of a functional gene of C. utilis as far as we know. The sequence of 2209 bp was an alignment of four restriction fragments determined separately. The sequences of HindIII-CluI, CluI-EcoRI, EcoRI-BglII and BglII-Hind111 fragments could be clearly read from ...
A nucleus-encoded chloroplast protein regulated by iron availability
... domain may be functionally important (Supplemental Table S1). Altogether, these observations suggest that TAA1 could be involved in RNA metabolism. A comparison of the cDNA sequences of the wild-type TAA1 and of the taa1 mutant, obtained by RT-PCR, revealed a single base substitution, which changes ...
... domain may be functionally important (Supplemental Table S1). Altogether, these observations suggest that TAA1 could be involved in RNA metabolism. A comparison of the cDNA sequences of the wild-type TAA1 and of the taa1 mutant, obtained by RT-PCR, revealed a single base substitution, which changes ...
Suppression mechanisms
... related genes is to begin with a strain that already contains a mutation affecting the pathway of interest, selecting for mutations that modify its phenotype. Modifiers that result in a more severe phenotype are termed enhancers, while mutations that restore a more wild-type phenotype despite the co ...
... related genes is to begin with a strain that already contains a mutation affecting the pathway of interest, selecting for mutations that modify its phenotype. Modifiers that result in a more severe phenotype are termed enhancers, while mutations that restore a more wild-type phenotype despite the co ...
Review THE SELECTION OF APTAMERS SPECIFIC FOR
... RNA molecules exhibit remarkable conformational flexibility and functional versatility [1]. RNA aptamers are single-stranded oligonucleotides composed of ca. 20 to 100 nucleotides. Their unique three-dimensional structures confer specificity for binding to the target: the Latin “aptus” means “to fit ...
... RNA molecules exhibit remarkable conformational flexibility and functional versatility [1]. RNA aptamers are single-stranded oligonucleotides composed of ca. 20 to 100 nucleotides. Their unique three-dimensional structures confer specificity for binding to the target: the Latin “aptus” means “to fit ...
Life and Chemistry: Large molecules: Proteins
... • Macromolecules are made from smaller monomers by means of a condensation or dehydration reaction in which an OH from one monomer is linked to an H from ...
... • Macromolecules are made from smaller monomers by means of a condensation or dehydration reaction in which an OH from one monomer is linked to an H from ...
A unifying model for mTORC1-mediated regulation of mRNA translation Please share
... overall protein synthesis and polysome profiles, selectively suppressed the translation of TOP mRNAs, without affecting mTORC1 activity (Fig. 4c-f). Importantly, in the DKO cells, eIF4G1 depletion also selectively repressed TOP mRNA translation (Fig. 4c,e,f), consistent with eIF4G1 acting downstream ...
... overall protein synthesis and polysome profiles, selectively suppressed the translation of TOP mRNAs, without affecting mTORC1 activity (Fig. 4c-f). Importantly, in the DKO cells, eIF4G1 depletion also selectively repressed TOP mRNA translation (Fig. 4c,e,f), consistent with eIF4G1 acting downstream ...
THE QUEST FOR A MESSAGE: BUDDING YEAST, A MODEL
... blockage at a particular step of splicing when its gene product is in excess. With these examples we intend to show that in yeast regulated splicing is sophisticated. This is highly relevant because due to the evolutionary conservation of the spliceosome, its regulation is likely to follow strategie ...
... blockage at a particular step of splicing when its gene product is in excess. With these examples we intend to show that in yeast regulated splicing is sophisticated. This is highly relevant because due to the evolutionary conservation of the spliceosome, its regulation is likely to follow strategie ...
Expression and V (D) J recombination activity of mutated RAG
... that was previously developed in this laboratory ([7]). A combination of plasmid DNAs can be delivered to recipient cells to provide the V(D)J reaction substrate as well as sources of RAG-1 and RAG-2. In fibroblasts, mRNA derived from expression of the endogenous RAG loci is not detectable ([2]), so ...
... that was previously developed in this laboratory ([7]). A combination of plasmid DNAs can be delivered to recipient cells to provide the V(D)J reaction substrate as well as sources of RAG-1 and RAG-2. In fibroblasts, mRNA derived from expression of the endogenous RAG loci is not detectable ([2]), so ...
Chapter 3 - McGraw Hill Higher Education
... – Proteins such as hemoglobin are responsible for the transport of substances within the body. – Proteins also transport substances across cell membranes. ...
... – Proteins such as hemoglobin are responsible for the transport of substances within the body. – Proteins also transport substances across cell membranes. ...
Amino Acids of the Sulfolobus solfataricus Mini-chromosome
... the wild type protein. DNA band shift assays were carried out with amounts of protein ranging from 160 to 1600 fmol on various DNA ligands, namely molecules containing a bubble of 20 T residues called Bub-20T molecules, blunt duplexes, and single-stranded oligonucleotides as indicated in Fig. 5A. Wi ...
... the wild type protein. DNA band shift assays were carried out with amounts of protein ranging from 160 to 1600 fmol on various DNA ligands, namely molecules containing a bubble of 20 T residues called Bub-20T molecules, blunt duplexes, and single-stranded oligonucleotides as indicated in Fig. 5A. Wi ...
Biochimica et Biophysica Acta
... specific solubilization of the additional cell envelope protein or of another cell envelope protein. The additional cell envelope protein is not heatmodifiable as the same electrophoretic mobility was observed after preincubation in sample buffer for 2 h at 37°C and after boiling for 5 min. Thus a p ...
... specific solubilization of the additional cell envelope protein or of another cell envelope protein. The additional cell envelope protein is not heatmodifiable as the same electrophoretic mobility was observed after preincubation in sample buffer for 2 h at 37°C and after boiling for 5 min. Thus a p ...
Identification of the nuclear localization signals within the Epstein
... was determined by DNA sequencing and immunoblotting. To ensure easy visualization of both the nucleus and cytoplasm the constructs were transfected into HeLa cells. All of the constructs were transiently expressed in HeLa cells and the cellular location of the fusion proteins was determined by confo ...
... was determined by DNA sequencing and immunoblotting. To ensure easy visualization of both the nucleus and cytoplasm the constructs were transfected into HeLa cells. All of the constructs were transiently expressed in HeLa cells and the cellular location of the fusion proteins was determined by confo ...
Proteins and Enzymes
... just like there is only one specific key which fits into a specific type of lock. ...
... just like there is only one specific key which fits into a specific type of lock. ...
Role of Ime4 Protein in PHO Regulon of S.cerevisiae.
... supported by its enrichment in the 3’UTR. Furthermore, Wang and group in their two studies published in 2014 have demonstrated that m6A not only plays a role in mRNA stability but also targets mRNA for degradation (Wang et al. 2014a; Wang et al. 2014b). In all organisms tested, deletion of methyltra ...
... supported by its enrichment in the 3’UTR. Furthermore, Wang and group in their two studies published in 2014 have demonstrated that m6A not only plays a role in mRNA stability but also targets mRNA for degradation (Wang et al. 2014a; Wang et al. 2014b). In all organisms tested, deletion of methyltra ...
Dicot and monocot plants differ in retinoblastoma
... genes, and the STRECHER program (http://bioweb.pasteur.fr/ seqanal/interfaces/stretcher.html) was used to identify the borders. The regions containing RBR-like protein sequences from the A pocket domain up to the C-terminus were aligned by using ClustalW (http://bioweb.pasteur.fr/seqanal/ClustalW.ph ...
... genes, and the STRECHER program (http://bioweb.pasteur.fr/ seqanal/interfaces/stretcher.html) was used to identify the borders. The regions containing RBR-like protein sequences from the A pocket domain up to the C-terminus were aligned by using ClustalW (http://bioweb.pasteur.fr/seqanal/ClustalW.ph ...
Mir-190b negatively contributes to the Trypanosoma cruzi
... viability of infected cells was examined at precise timepoints after parasitic interaction (0 h, 2 h, 6 h, 12 h, 24 h, and 48 h), and uninfected cells were used as control in the assays. A 58.4% reduction in cellular viability at 48 h p.i. was observed (Fig. 1B). In addition, uninfected cells were t ...
... viability of infected cells was examined at precise timepoints after parasitic interaction (0 h, 2 h, 6 h, 12 h, 24 h, and 48 h), and uninfected cells were used as control in the assays. A 58.4% reduction in cellular viability at 48 h p.i. was observed (Fig. 1B). In addition, uninfected cells were t ...
april7_maindocument_jvirol
... structure with a diameter of approximately 10 nm and up to several hundreds of nm in length (33, 34). The highly basic N protein has a molecular mass ranging between 45 and 60 kDa in the various groups of coronaviruses and, along with its coding RNA, is synthesized in large amounts during infection ...
... structure with a diameter of approximately 10 nm and up to several hundreds of nm in length (33, 34). The highly basic N protein has a molecular mass ranging between 45 and 60 kDa in the various groups of coronaviruses and, along with its coding RNA, is synthesized in large amounts during infection ...
X-ray structures of the N and C-terminal domains of a
... structure with a diameter of approximately 10 nm and up to several hundreds of nm in length (33, 34). The highly basic N protein has a molecular mass ranging between 45 and 60 kDa in the various groups of coronaviruses and, along with its coding RNA, is synthesized in large amounts during infection ...
... structure with a diameter of approximately 10 nm and up to several hundreds of nm in length (33, 34). The highly basic N protein has a molecular mass ranging between 45 and 60 kDa in the various groups of coronaviruses and, along with its coding RNA, is synthesized in large amounts during infection ...
Lab - Week One: The Scientific Method
... b) “Synthesize” a pool of amino acids by writing the three letter abbreviation for each polar amino acid on a separate rectangular yellow post-it note and each non-polar amino acid on a separate rectangular grey/purplish post-it note. (A table of polar and non-polar amino acids will be provided for ...
... b) “Synthesize” a pool of amino acids by writing the three letter abbreviation for each polar amino acid on a separate rectangular yellow post-it note and each non-polar amino acid on a separate rectangular grey/purplish post-it note. (A table of polar and non-polar amino acids will be provided for ...
Biotech Patenting in India - PHARMACEUTICALS EXPORT
... US patent law grants patent to microorganisms when modified by human intervention (Diamond v Chakraborty, US Supr. Court, 1980) EPO has similar law with regard to patenting microorganisms Patentability Criteria – Novelty, Inventive step (Non-Obvious in US), Industrial applicability (Utility in ...
... US patent law grants patent to microorganisms when modified by human intervention (Diamond v Chakraborty, US Supr. Court, 1980) EPO has similar law with regard to patenting microorganisms Patentability Criteria – Novelty, Inventive step (Non-Obvious in US), Industrial applicability (Utility in ...
Whey to isolate - Proteinfactory
... Glycomacropeptide triggers hormones that may signal fullness and act as a satiety peptide. As a result, MF WPI could be used in a weight-management product. Formulators using WPI need to understand these two major types of WPI and examine their functional differences in specific applications, such a ...
... Glycomacropeptide triggers hormones that may signal fullness and act as a satiety peptide. As a result, MF WPI could be used in a weight-management product. Formulators using WPI need to understand these two major types of WPI and examine their functional differences in specific applications, such a ...
Gene expression
Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses - to generate the macromolecular machinery for life.Several steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-translational modification of a protein. Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism.In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, i.e. observable trait. The genetic code stored in DNA is ""interpreted"" by gene expression, and the properties of the expression give rise to the organism's phenotype. Such phenotypes are often expressed by the synthesis of proteins that control the organism's shape, or that act as enzymes catalysing specific metabolic pathways characterising the organism.