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Proteomics of spermatogenesis: from protein lists to understanding
... work, testes from 8-, 18- and 45-day-old postnatal mice were comparatively studied and 44 proteins or variant forms were identified. However, these time points representing the first wave of spermatogenesis were not exact enough and this time span may have been too large. So, additional time points ...
... work, testes from 8-, 18- and 45-day-old postnatal mice were comparatively studied and 44 proteins or variant forms were identified. However, these time points representing the first wave of spermatogenesis were not exact enough and this time span may have been too large. So, additional time points ...
Supplemental Text
... In the equation for sp in (2), η is the fraction of ribosomes that translate ribosomal protein and kp = kp0 fres is the rate of protein synthesis per ribosome. The function fres (a/V ) describes the increase of the translation rate kp as a function of the intracellular resource concentration a/V . F ...
... In the equation for sp in (2), η is the fraction of ribosomes that translate ribosomal protein and kp = kp0 fres is the rate of protein synthesis per ribosome. The function fres (a/V ) describes the increase of the translation rate kp as a function of the intracellular resource concentration a/V . F ...
An Enhancer Trap Screen for Ecdysone
... blocks this process (Fekete et al. 1975). A role for proteases during leg disc elongation is further supported by the observation that prepupal leg discs make and secrete proteases in response to ecdysone in culture (Poodry and Schneiderman 1971; Fekete et al. 1975; PinoHeiss and Schubiger 1989). Fa ...
... blocks this process (Fekete et al. 1975). A role for proteases during leg disc elongation is further supported by the observation that prepupal leg discs make and secrete proteases in response to ecdysone in culture (Poodry and Schneiderman 1971; Fekete et al. 1975; PinoHeiss and Schubiger 1989). Fa ...
MagNA Lyser Instrument
... Rarely expressed targets in small numbers of target cells, as seen in experiments about minimal residual diseases, are difficult to detect. Increasing the cell number can improve sensitivity and lead to accurate results. Without the MagNA Lyser preprocessing, the MagNA Pure mRNA HS Kit can efficient ...
... Rarely expressed targets in small numbers of target cells, as seen in experiments about minimal residual diseases, are difficult to detect. Increasing the cell number can improve sensitivity and lead to accurate results. Without the MagNA Lyser preprocessing, the MagNA Pure mRNA HS Kit can efficient ...
Latent Periodicity of Many Genes
... Development of mathematical methods for study of symbolical sequence periodicity gets special significance nowadays. First of all it is concerned with the successful determination of DNA sequences from various genomes and accumulation of a great number of amino acid sequences. Therefore there is a pr ...
... Development of mathematical methods for study of symbolical sequence periodicity gets special significance nowadays. First of all it is concerned with the successful determination of DNA sequences from various genomes and accumulation of a great number of amino acid sequences. Therefore there is a pr ...
Amino Acids - Building Blocks of Proteins
... recognized that the structure of a finch’s beak was related to the food it ate. This fundamental structure-function relationship is also true at all levels below the Potassium macro level, including proteins and other structures at the molecular Ion level. For two examples of proteins and their func ...
... recognized that the structure of a finch’s beak was related to the food it ate. This fundamental structure-function relationship is also true at all levels below the Potassium macro level, including proteins and other structures at the molecular Ion level. For two examples of proteins and their func ...
Authors` pre-proof version - University of Connecticut
... aaRS are universally distributed within the three domains (with the exception of some aaRS requiring tRNA-dependent amino acid synthesis, and domain-specific class I and class II LysRS families (Ibba et al. 1997), many, if not most of these groups have undergone horizontal gene transfer across all t ...
... aaRS are universally distributed within the three domains (with the exception of some aaRS requiring tRNA-dependent amino acid synthesis, and domain-specific class I and class II LysRS families (Ibba et al. 1997), many, if not most of these groups have undergone horizontal gene transfer across all t ...
DNA
... DNA recombination The process that two DNA molecules from different source join together by covalent bond to form a new DNA molecule is called DNA recombination. Recombinant DNA DNA recombination technique By the application of some tool enzymes, the target gene and vector are ligated together, the ...
... DNA recombination The process that two DNA molecules from different source join together by covalent bond to form a new DNA molecule is called DNA recombination. Recombinant DNA DNA recombination technique By the application of some tool enzymes, the target gene and vector are ligated together, the ...
Subunit Isoform of X,K-ATPase in Human Skeletal Muscle
... bility of the detected  m protein without deglycosylation corresponds to an apparent molecular mass of 57 kDa (Fig. 3, lane 4). Treatment with either Endo H or PNGase F shifted the band mobility indicating that N-glycans on human skeletal muscle  m are sensitive to these glycosidases (Fig. 3, lane ...
... bility of the detected  m protein without deglycosylation corresponds to an apparent molecular mass of 57 kDa (Fig. 3, lane 4). Treatment with either Endo H or PNGase F shifted the band mobility indicating that N-glycans on human skeletal muscle  m are sensitive to these glycosidases (Fig. 3, lane ...
- Wiley Online Library
... to light decreased transcript levels in ME cells (Lai et al., 2002). Interestingly, cis-elements that affect BS-specific expression of ME are different for F. bidentis and a close C4 relative, F. trinervia. In F. bidentis, the endogenous NADPME gene may require elements within the coding region at t ...
... to light decreased transcript levels in ME cells (Lai et al., 2002). Interestingly, cis-elements that affect BS-specific expression of ME are different for F. bidentis and a close C4 relative, F. trinervia. In F. bidentis, the endogenous NADPME gene may require elements within the coding region at t ...
Comparison of modeling options for the mRNA Life cycle
... transcription process, which produces copies of the genetic information in the form of mRNA molecules. The mRNA molecules are then transcribed into proteins, which are the machines that carry out all the functions in a living organism. The level of the single proteins is what determines the phenotyp ...
... transcription process, which produces copies of the genetic information in the form of mRNA molecules. The mRNA molecules are then transcribed into proteins, which are the machines that carry out all the functions in a living organism. The level of the single proteins is what determines the phenotyp ...
Proteins - e
... lock (Fischer's lock and key hypothesis). Both the enzyme and the substrate were seen as rigid structures, with the substrate (the key) fitting perfectly into the active site (the lock). However, this scenario does not explain how some enzymes can catalyse a reaction on a range of different substrat ...
... lock (Fischer's lock and key hypothesis). Both the enzyme and the substrate were seen as rigid structures, with the substrate (the key) fitting perfectly into the active site (the lock). However, this scenario does not explain how some enzymes can catalyse a reaction on a range of different substrat ...
Cystic Fibrosis and CFTR Gene - Atlas of Genetics and Cytogenetics
... VI-3.1. Class 1: mutations altering the production of the protein. These mutations result in the total or partial absence of the protein. This class includes the nonsense mutations and those that produce a premature stop codon (anomalies of splicing and frameshift mutations). In certain cases the m ...
... VI-3.1. Class 1: mutations altering the production of the protein. These mutations result in the total or partial absence of the protein. This class includes the nonsense mutations and those that produce a premature stop codon (anomalies of splicing and frameshift mutations). In certain cases the m ...
Directed Evolution of ATP Binding Proteins from a Zinc Finger
... A number of different display methods have been employed in combination with new scaffolds to evolve interesting and useful new proteins [9, 11, 15–19]. Phage display has been the most popular method for selecting functional molecules from new scaffolds; the following are examples of successful expe ...
... A number of different display methods have been employed in combination with new scaffolds to evolve interesting and useful new proteins [9, 11, 15–19]. Phage display has been the most popular method for selecting functional molecules from new scaffolds; the following are examples of successful expe ...
Mechanism of Translation
... C) They are not recognized by any tRNA molecules. D) Their conformations do not allow them to fit properly in the A site of the ribosome. ...
... C) They are not recognized by any tRNA molecules. D) Their conformations do not allow them to fit properly in the A site of the ribosome. ...
Methods for distinguishing between protein-coding and
... (lncRNAs), which closely resemble mRNAs in that they possess an m7Gpppn cap at the 5' end, a poly(A) tail at the 3' end, and in most-cases undergo splicing [14]. The expression of many lncRNAs has been shown to be altered in a wide range of human diseases, including cancer [15], which promotes the i ...
... (lncRNAs), which closely resemble mRNAs in that they possess an m7Gpppn cap at the 5' end, a poly(A) tail at the 3' end, and in most-cases undergo splicing [14]. The expression of many lncRNAs has been shown to be altered in a wide range of human diseases, including cancer [15], which promotes the i ...
Nomenclature of the ARID family of DNA
... ARID1A and ARID1B are 80% identical within the ARID and approximately 50% identical across their full-length amino acid sequences, although ARID1A has additional glutamine-rich regions and several LXXLL motifs (presumptive nuclear hormone receptor-binding sites) that are not precisely conserved in A ...
... ARID1A and ARID1B are 80% identical within the ARID and approximately 50% identical across their full-length amino acid sequences, although ARID1A has additional glutamine-rich regions and several LXXLL motifs (presumptive nuclear hormone receptor-binding sites) that are not precisely conserved in A ...
PowerPoint (click here)
... At least some of the residues that are important for ubiquitin ligase activity (452, 455, and 457) are conserved in the mystery protein. The one residue that we checked that was not conserved (374) seems to be less important for activity. These results are consistent with the possibility that the my ...
... At least some of the residues that are important for ubiquitin ligase activity (452, 455, and 457) are conserved in the mystery protein. The one residue that we checked that was not conserved (374) seems to be less important for activity. These results are consistent with the possibility that the my ...
Paper
... Li et al. (Research Articles, 1 July 2011, p. 53; published online 19 May 2011) reported more than 10,000 mismatches between messenger RNA and DNA sequences from the same individuals, which they attributed to previously unrecognized mechanisms of gene regulation. We found that at least 88% of these ...
... Li et al. (Research Articles, 1 July 2011, p. 53; published online 19 May 2011) reported more than 10,000 mismatches between messenger RNA and DNA sequences from the same individuals, which they attributed to previously unrecognized mechanisms of gene regulation. We found that at least 88% of these ...
Functional genomics analysis of foliar condensed tannin and
... SCOTT A. HARDING,1 HONGYING JIANG,1,2 MIJEONG LEE JEONG,1,3 FANNY L. CASADO,1,4 HAN-WEI LIN1 and CHUNG-JUI TSAI1,5 ...
... SCOTT A. HARDING,1 HONGYING JIANG,1,2 MIJEONG LEE JEONG,1,3 FANNY L. CASADO,1,4 HAN-WEI LIN1 and CHUNG-JUI TSAI1,5 ...
Method and system for computationally identifying clusters within a
... are mixed With the DNA-binding proteins, the DNA-binding proteins bind to speci?c oligonucleotide duplexes that con tain base-pair sequences that the DNA-binding proteins recogniZe; or, in other Words, a particular type of DNA binding protein binds to those oligonucleotide duplexes that ...
... are mixed With the DNA-binding proteins, the DNA-binding proteins bind to speci?c oligonucleotide duplexes that con tain base-pair sequences that the DNA-binding proteins recogniZe; or, in other Words, a particular type of DNA binding protein binds to those oligonucleotide duplexes that ...
Table S6: Domains present in the primary network generated from
... is thought interact with FtsZ. Thought to catalyse 2-phosphosulpholactate = sulpholactate + phosphate. Probable magnesium cofactor. Involved in the second step of coenzyme M biosynthesis. Inhibited by vanadate in Methanococcus jannaschii. Also known as the ComB family. CbiD is essential for cobalami ...
... is thought interact with FtsZ. Thought to catalyse 2-phosphosulpholactate = sulpholactate + phosphate. Probable magnesium cofactor. Involved in the second step of coenzyme M biosynthesis. Inhibited by vanadate in Methanococcus jannaschii. Also known as the ComB family. CbiD is essential for cobalami ...
Gene expression
Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses - to generate the macromolecular machinery for life.Several steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-translational modification of a protein. Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism.In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, i.e. observable trait. The genetic code stored in DNA is ""interpreted"" by gene expression, and the properties of the expression give rise to the organism's phenotype. Such phenotypes are often expressed by the synthesis of proteins that control the organism's shape, or that act as enzymes catalysing specific metabolic pathways characterising the organism.