Chapter Objectives: Chapter 20 Biotechnology
... Project 15. Describe how recombinant DNA technology can have medical applications such as diagnosis of genetic disease, development of gene therapy, vaccine production, and development of pharmaceutical products 16. Describe how gene manipulation has practical applications for agriculture 17. Descri ...
... Project 15. Describe how recombinant DNA technology can have medical applications such as diagnosis of genetic disease, development of gene therapy, vaccine production, and development of pharmaceutical products 16. Describe how gene manipulation has practical applications for agriculture 17. Descri ...
Recombinant DNA and gene cloning To use an unique feature(s) of
... partial sequence of the gene (based on protein sequence) activity (enzyme activity) genetic defect (temperature/chemical sensitivity) Strategy: 1) break up the DNA; 2) separated each fragement into a unique locations (library); 3) screen your gene out from the library. Tools 1) restriction endonucle ...
... partial sequence of the gene (based on protein sequence) activity (enzyme activity) genetic defect (temperature/chemical sensitivity) Strategy: 1) break up the DNA; 2) separated each fragement into a unique locations (library); 3) screen your gene out from the library. Tools 1) restriction endonucle ...
The Cell Cycle
... G1-S Checkpoint And S-Phase Considerations • Replication should not begin if DNA is damaged • DNA integrity monitored by – p53 induced genes ...
... G1-S Checkpoint And S-Phase Considerations • Replication should not begin if DNA is damaged • DNA integrity monitored by – p53 induced genes ...
• - cloudfront.net
... 22. What are the three parts of a nucleotide? When nucleotides join together what type of polymers do they form? 23. How does base pairing affect the amount of adenine, thymine, guanine, and cytosine in DNA? 24. Where and in what form does DNA exist in eukaryotes? in prokaryotes? 25. What is the res ...
... 22. What are the three parts of a nucleotide? When nucleotides join together what type of polymers do they form? 23. How does base pairing affect the amount of adenine, thymine, guanine, and cytosine in DNA? 24. Where and in what form does DNA exist in eukaryotes? in prokaryotes? 25. What is the res ...
RNA
... pentose residue. Depending on a pentose structure are distinguished ribonucleotides and deoxyribonucleotides. Nucleotides is possible to consider, on the one hand, as ethers of nucleosides (their phosphates), and on the other hand - as acids, in connection with presence in their structure of the res ...
... pentose residue. Depending on a pentose structure are distinguished ribonucleotides and deoxyribonucleotides. Nucleotides is possible to consider, on the one hand, as ethers of nucleosides (their phosphates), and on the other hand - as acids, in connection with presence in their structure of the res ...
A CAAT–Box Binding Factor Gene That Regulates Seed Development
... - The CCAAT box is one of the most wide spread promoter elements, being present in 25% of eukaryotic promoters - to date, the one protein that binds and activates the CCAAT box is nuclear factor Y (NF-Y), a trimer composed of distinct subunits: NF-YA, NF-YB and NF-YC. NF-YB-NF-YC belong to the class ...
... - The CCAAT box is one of the most wide spread promoter elements, being present in 25% of eukaryotic promoters - to date, the one protein that binds and activates the CCAAT box is nuclear factor Y (NF-Y), a trimer composed of distinct subunits: NF-YA, NF-YB and NF-YC. NF-YB-NF-YC belong to the class ...
Document
... relatively easy to extract from cells and tissues. – DNA molecules from most organisms are much too large to be analyzed, so they must first be cut into smaller pieces. – Many bacteria produce restriction enzymes that cut DNA molecules into precise pieces, called restriction fragments that are sever ...
... relatively easy to extract from cells and tissues. – DNA molecules from most organisms are much too large to be analyzed, so they must first be cut into smaller pieces. – Many bacteria produce restriction enzymes that cut DNA molecules into precise pieces, called restriction fragments that are sever ...
How many nucleotides are in 12 mRNA codons?
... The first amino acid will be wrong, but the last three will be correct. ...
... The first amino acid will be wrong, but the last three will be correct. ...
Fig. 16.19b
... because one parental strand is oriented 3’->5’ into the fork, while the other antiparallel parental strand is oriented 5’->3’ into the fork. • At the replication fork, one parental strand (3’-> 5’ into the fork), the leading strand, can be used by polymerases as a template for a continuous ...
... because one parental strand is oriented 3’->5’ into the fork, while the other antiparallel parental strand is oriented 5’->3’ into the fork. • At the replication fork, one parental strand (3’-> 5’ into the fork), the leading strand, can be used by polymerases as a template for a continuous ...
Chapter 13: The Genetic Code and Transcription
... Transcription results in the synthesis of a single-stranded RNA molecule complementary to only one of the two DNA strands. The initial step is called template binding; in it, RNA polymerase subunits recognize specific DNA sequences called promoters. The helix becomes denatured and unwound locally, ...
... Transcription results in the synthesis of a single-stranded RNA molecule complementary to only one of the two DNA strands. The initial step is called template binding; in it, RNA polymerase subunits recognize specific DNA sequences called promoters. The helix becomes denatured and unwound locally, ...
Nucleic acids and protein synthesis
... DNA double helix is unzipped by an enzyme called a helicase. Helicase breaks hydrogen bonds linking the nitrogen bases. Occurs at the replication forks of the double helix. At the replication fork; an enzyme called DNA polymerase moves along the strands, reading the nitrogen base of each nucleotid ...
... DNA double helix is unzipped by an enzyme called a helicase. Helicase breaks hydrogen bonds linking the nitrogen bases. Occurs at the replication forks of the double helix. At the replication fork; an enzyme called DNA polymerase moves along the strands, reading the nitrogen base of each nucleotid ...
No Slide Title
... HG are more complex, with made alternative splicing generating a large number of protein products. Less than 2% of the genome codes for proteins. Almost all (99.9%) DNA sequences are exactly the same in all people. The functions are unknown for over 50% of discovered genes W. Tassaneeyakul ...
... HG are more complex, with made alternative splicing generating a large number of protein products. Less than 2% of the genome codes for proteins. Almost all (99.9%) DNA sequences are exactly the same in all people. The functions are unknown for over 50% of discovered genes W. Tassaneeyakul ...
Guided Exploration- (RI3) Learning Goal Three: Explain how DNA is
... The workers have assistants fetch the correct supplies in the kingdom. Then they read the instructions, and put the supplies together just like the instructions said. When the workers were finished, they had a beautiful castle before them and were happy that they had done such a good job. DNA, Trans ...
... The workers have assistants fetch the correct supplies in the kingdom. Then they read the instructions, and put the supplies together just like the instructions said. When the workers were finished, they had a beautiful castle before them and were happy that they had done such a good job. DNA, Trans ...
Honors Biology Midterm Study Guide Chapter 1 and 2: The Science
... 2. DNA replication: what is it, when does it occur in the cell cycle, why does it occur, how does it occur? Replicate the strand of DNA above: ____________________________________________________ 3. Transcription and translation: how is genetic information encoded in DNA transcribed (copied) as ...
... 2. DNA replication: what is it, when does it occur in the cell cycle, why does it occur, how does it occur? Replicate the strand of DNA above: ____________________________________________________ 3. Transcription and translation: how is genetic information encoded in DNA transcribed (copied) as ...
DNA Packaging and Ch..
... An organism’s task in managing its DNA: 1.) Efficient packaging and storage, to fit into very small spaces (2.3 million times smaller) 2.) Requires “de-packaging” of DNA to access correct genes at the correct time (gene expression). 3.) Accurate DNA replication during the Sphase of the cell-cycle. ...
... An organism’s task in managing its DNA: 1.) Efficient packaging and storage, to fit into very small spaces (2.3 million times smaller) 2.) Requires “de-packaging” of DNA to access correct genes at the correct time (gene expression). 3.) Accurate DNA replication during the Sphase of the cell-cycle. ...
JF lect 5 12
... 1910 – Morgan – the “white” eye color gene of Drosophila is located on the X-chromosome - there are many other X-linked genes ...
... 1910 – Morgan – the “white” eye color gene of Drosophila is located on the X-chromosome - there are many other X-linked genes ...
Chap 8-11, pt 2 Mendel through Biotechnology
... profile murder case in which PCR and DNA fingerprinting play a prominent but unpersuasive role. It did bring the technology into the ...
... profile murder case in which PCR and DNA fingerprinting play a prominent but unpersuasive role. It did bring the technology into the ...
Genetics checklist
... Describe gene and chromosomal mutations in the DNA sequence. Explain how gene and chromosomal mutations may or may not result in a phenotypic change. Explain the basic processes of transcription and/or translation, and their roles in the expression of genes. Explain that the basic components of DNA ...
... Describe gene and chromosomal mutations in the DNA sequence. Explain how gene and chromosomal mutations may or may not result in a phenotypic change. Explain the basic processes of transcription and/or translation, and their roles in the expression of genes. Explain that the basic components of DNA ...
CP Biology 9.2 Copying DNA PCR uses polymerase to copy DNA
... DNA polymerase is a key enzyme in DNA replication. PCR also uses DNA polymerase to make copies of DNA – but in a test tube, not in a cell. ...
... DNA polymerase is a key enzyme in DNA replication. PCR also uses DNA polymerase to make copies of DNA – but in a test tube, not in a cell. ...
Replisome
The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.