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coding region of DNA. o Introns – non
coding region of DNA. o Introns – non

... o Tissue-specific transcription factors. o Repressors present in some regions and absent in others. Elongation (step 2 of transcription). o RNA polymerase breaks interactions with transcription factors and escapes the promoter region to start elongation. o RNA polymerase moves along the DNA template ...
FREE Sample Here
FREE Sample Here

... b. the statement that genes are perpetuated as nucleic acid, but function in the form of protein. c. the relationship between a nucleotide sequence of DNA and the corresponding gene sequence of DNA. d. the relationship between a three-nucleotide sequence of mRNA and the corresponding amino acid. ...
Biotechnology - Genetic Engineering
Biotechnology - Genetic Engineering

... Restriction Enzymes: These are enzymes naturally occurring in bacteria. The bacteria use these enzymes to protect themselves from viruses invading them. Restriction enzymes “cut” the DNA the virus injects into them so that the virus cannot be reproduced. Act like “molecular scissors”. Scientist use ...
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Plasmid modeling Use beads to demonstrate how a gene is

... to the insulin produced in a human pancreas. How is this possible? ...
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Protein Synthesis

... to make proteins) ...
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Translation Worksheet

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DNA Replication
DNA Replication

... found in the nucleus of every cell  DNA and proteins make up chromosomes – contain traits  sections of it make up genes ...
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lecture4(GS351)

... How do RNA polymerases know where to begin transcription and which way to go? In bacteria RNA polymerase binds specific sequences near the start site of transcription that orient the polymerase: ...
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... A research technique developed to move rapidly up or down a chromosome in search of a particular gene. It enables researchers to make large jumps over uninformative regions of DNA. Chromosome jumping is used to bypass regions difficult to clone, such as those containing repetitive DNA, that cannot b ...
Bio 6B Lecture Slides - D
Bio 6B Lecture Slides - D

... origins of replication, where the two strands are separated •  A bacterial chromosome typically has one replication origin •  A eukaryotic chromosome may have hundreds or even thousands of replication origins Origin of replication ...
GENETICS EOCT STUDY GUIDE 1. DNA Bases: Guanine RNA
GENETICS EOCT STUDY GUIDE 1. DNA Bases: Guanine RNA

... 7. Inversions in chromosomes occur when part of a chromosome breaks out and is reinserted upside down. Which of the diagrams below represents an inversion? ...
DNA - The Double Helix - BIOLOGY
DNA - The Double Helix - BIOLOGY

... Recall that the nucleus is a small spherical, dense body in a cell. It is often called the "control center" because it controls all the activities of the cell including cell reproduction, and heredity. Chromosomes are microscopic, threadlike strands composed of the chemical DNA (short for deoxyribon ...
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... (a) A hypersensitive site is a region of eukaryotic DNA that is being associated with actively transcribed DNA and is unusually sensitive to degradation by nucleases. (b) Enhancers are DNA sequences that regulate the transcription of certain genes by allowing binding of specific transactivator prote ...
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Chapter 6

session 10 - E-Learning/An-Najah National University
session 10 - E-Learning/An-Najah National University

... its two nucleotide chains (Figure 3.14). Each nucleotide strand then serves as a template, or set of instructions, for building a new nucleotide strand. Remember that nucleotides join in a complementary way: adenine (A) always bonds to thymine (T), and guanine (G) always bonds to cytosine (C). Hence ...
GENETICS EOCT STUDY GUIDE 1. DNA Bases: Guanine RNA
GENETICS EOCT STUDY GUIDE 1. DNA Bases: Guanine RNA

... 7. Inversions in chromosomes occur when part of a chromosome breaks out and is reinserted upside down. Which of the diagrams below represents an inversion? ...
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From DNA to Protein

... Science Standards (NH Science Curriculum Frameworks) S:LS3:8:3:1 Recognize that hereditary information is contained in genes, which are located in the chromosomes of each cell; and explain that inherited traits can be determined by either one or many genes, and that a single gene can influence more ...
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Biotechnology and Genetic Engineering

... A new DNA sequence created when the DNA of one organism is inserted into the DNA of another organism. This “new combination” of DNA is known as recombinant DNA. ...
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The Cell Cycle

... while the other chromatid moves to the opposite side. The cell becomes stretched out as they pull apart. ...
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Quick Guide for Forensic DNA Fingerprinting Kit BioRad Student

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... 14. Complementary base pairing is the paired relationship between purines and pyrimidines in DNA: A is hydrogen-bonded to T and G is hydrogen-bonded to C. 12.2 Replication of DNA 1. DNA replication is the process of copying a DNA molecule. Replication is semiconservative, with each strand of the ori ...
Final exam study guide
Final exam study guide

... if you know what the sequence of bases are on one strand you can predict the sequence of bases on the “complementary” strand based on these ...
DNA - Mrs. Barrett`s Biology Site
DNA - Mrs. Barrett`s Biology Site

... distinguish that DNA from other DNA.  DNA is extracted from cells e.g. blood or semen by breaking up the cell membrane.  DNA amplification can be used if the quantity of DNA is low. Increasing the quantity is done by a technique called the polymerase chain reaction (PCR).  Restriction enzymes are ...
Transcription Study Guide
Transcription Study Guide

Pfu DNA Polymerase - G
Pfu DNA Polymerase - G

... the incorporation of 10 nanomoles of deoxyribonucleotides into acid-insoluble material in 30 minutes at 70°C using herring sperm DNA as a substrate. ...
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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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