Chapter 2 - rci.rutgers.edu
... (i) Denaturing: Two strands of DNA are unwound and separated by heating (ii) Annealing: primers - short strands of single-stranded DNA that match the sequences at either end of the target DNA, are bound to their complementary bases on the now single-stranded DNA. (iii) Polymerase: an enzyme whose jo ...
... (i) Denaturing: Two strands of DNA are unwound and separated by heating (ii) Annealing: primers - short strands of single-stranded DNA that match the sequences at either end of the target DNA, are bound to their complementary bases on the now single-stranded DNA. (iii) Polymerase: an enzyme whose jo ...
101 -- 2006
... b) Replication occurs as each base is paired with another exactly like it. c) The process is known as semiconservative replication because one old strand is conserved in the new molecule. d) The enzyme that catalyzes DNA replication is DNA polymerase. e) Complementary base pairs are held together wi ...
... b) Replication occurs as each base is paired with another exactly like it. c) The process is known as semiconservative replication because one old strand is conserved in the new molecule. d) The enzyme that catalyzes DNA replication is DNA polymerase. e) Complementary base pairs are held together wi ...
Analysis of Gene Sequences
... dTTP (dNTPs). All DNA polymerases have two fundamental properties in common. (1) New DNA is synthesized only by elongation of an existing strand at its 3’ end. (2) Synthesis requires nucleotide precursors, a free 3’ OH end, and a template strand. A general substrate for DNA polymerase looks like thi ...
... dTTP (dNTPs). All DNA polymerases have two fundamental properties in common. (1) New DNA is synthesized only by elongation of an existing strand at its 3’ end. (2) Synthesis requires nucleotide precursors, a free 3’ OH end, and a template strand. A general substrate for DNA polymerase looks like thi ...
Organic Molecules Proteins: The Workhorses of Life Carbohydrates
... • Unraveling the Past: Mitochondrial DNA ...
... • Unraveling the Past: Mitochondrial DNA ...
Bio1A Unit 1-2 Biological Molecules Notes File
... interact with other molecules or itself. • Cysteine (R = -SH) can form a disulfide bond (covalent, rare) • Other side chains will interact through hydrogen (primary) ionic bonding • Ultimate structure is typically most thermodynamically stable (best fit) • Driven by interaction with H2O envirnoment ...
... interact with other molecules or itself. • Cysteine (R = -SH) can form a disulfide bond (covalent, rare) • Other side chains will interact through hydrogen (primary) ionic bonding • Ultimate structure is typically most thermodynamically stable (best fit) • Driven by interaction with H2O envirnoment ...
Genetics - Mobile County Public Schools
... Explain the structure of eukaryotic chromosomes, including transposons, introns, and exons. Compare spermatogenesis and oogenesis using charts. Describe occurrences and effects of sex linkage, autosomal linkage, crossover, multiple alleles, and polygenes Describe the structure and function of DNA, i ...
... Explain the structure of eukaryotic chromosomes, including transposons, introns, and exons. Compare spermatogenesis and oogenesis using charts. Describe occurrences and effects of sex linkage, autosomal linkage, crossover, multiple alleles, and polygenes Describe the structure and function of DNA, i ...
VII. Molecular Biology Techniques
... Northern blots allow investigators to determine the molecular weight of an mRNA and to measure relative amounts of the mRNA present in different samples. RNA (either total RNA or just mRNA) is separated by gel electrophoresis, usually an agarose gel. Because there are so many different RNA molecules ...
... Northern blots allow investigators to determine the molecular weight of an mRNA and to measure relative amounts of the mRNA present in different samples. RNA (either total RNA or just mRNA) is separated by gel electrophoresis, usually an agarose gel. Because there are so many different RNA molecules ...
Chapter 16 - HomeworkForYou
... based on X-rays and chemistry of DNA. • Only a pyrimidine-purine pair produces the 2-nm diameter indicated by the X-ray data. • Watson built a model in which the backbones were antiparallel (their subunits run in opposite directions). • The ladder forms a full turn of the helix every ten base pairs, ...
... based on X-rays and chemistry of DNA. • Only a pyrimidine-purine pair produces the 2-nm diameter indicated by the X-ray data. • Watson built a model in which the backbones were antiparallel (their subunits run in opposite directions). • The ladder forms a full turn of the helix every ten base pairs, ...
Molecular genetics of gene expression
... restriction digestion and ligation technology. 3. Describe a novel strategy to generate a T-DNA vector that allows the expression of several genes from a single position in the genome. 4. Discuss the advantages and disadvantages of using plastid vectors for plant transformation and gene expression. ...
... restriction digestion and ligation technology. 3. Describe a novel strategy to generate a T-DNA vector that allows the expression of several genes from a single position in the genome. 4. Discuss the advantages and disadvantages of using plastid vectors for plant transformation and gene expression. ...
DNA: The Molecule Of Life
... -Avery et al. discovered that DNA was the transforming principle in bacteria in 1944 -Hershey and Chase showed that DNA is the genetic material in 1952 -James Watson and Francis crick elucidated the structure of DNA in 1953. ...
... -Avery et al. discovered that DNA was the transforming principle in bacteria in 1944 -Hershey and Chase showed that DNA is the genetic material in 1952 -James Watson and Francis crick elucidated the structure of DNA in 1953. ...
Replication is when DNA
... B. Fill in the base-pairing rule as it applies to making RNA from DNA: Bases found in DNA Complementary bases in RNA G C T A C. Follow the directions by matching up the appropriate RNA bases with the single strand of DNA (click & drag). D. When finished matching the bases on the computer screen, co ...
... B. Fill in the base-pairing rule as it applies to making RNA from DNA: Bases found in DNA Complementary bases in RNA G C T A C. Follow the directions by matching up the appropriate RNA bases with the single strand of DNA (click & drag). D. When finished matching the bases on the computer screen, co ...
BIOLOGY Cells Unit GUIDE SHEET
... 2. What are the risks and benefits of altering the genes of existing organisms? STUDENTS WILL BE ABLE TO: 1. Evaluate the use of various techniques for manipulating DNA. 2. Explain how recombinant DNA techniques are used to genetically modify organisms (genetic engineering). 3. Evaluate the risks an ...
... 2. What are the risks and benefits of altering the genes of existing organisms? STUDENTS WILL BE ABLE TO: 1. Evaluate the use of various techniques for manipulating DNA. 2. Explain how recombinant DNA techniques are used to genetically modify organisms (genetic engineering). 3. Evaluate the risks an ...
Transcription Biology Review
... • All proteins interact weakly with DNA • Proteins with projecting amino acids interact with the DNA major groove • Hydrogen bonds stabilize position of proteins on DNA • Proteins that line up several amino acid contacts bind strongly to specific DNA sequences ...
... • All proteins interact weakly with DNA • Proteins with projecting amino acids interact with the DNA major groove • Hydrogen bonds stabilize position of proteins on DNA • Proteins that line up several amino acid contacts bind strongly to specific DNA sequences ...
Carrying Information in DNA
... The code within DNA is a series of codons (triplets), which encodes for specific amino acids. Amino acids then link together to form proteins. ...
... The code within DNA is a series of codons (triplets), which encodes for specific amino acids. Amino acids then link together to form proteins. ...
F plasmid
... 2. Replication of bacterial genome requires several enzymes: - Replication origin (oriC), a specific sequence in the chromosome - Helicase, unwind DNA at the origin - Primase, synthesize primers to start the process - DNA polymerase, synthesize a copy of DNA ...
... 2. Replication of bacterial genome requires several enzymes: - Replication origin (oriC), a specific sequence in the chromosome - Helicase, unwind DNA at the origin - Primase, synthesize primers to start the process - DNA polymerase, synthesize a copy of DNA ...
AP Biology: Unit 3A Homework
... 9. Make a list of the enzymes involved in replication and their roles. 10. Why does the DNA have to add nucleotides in the 5’ to 3’ direction? 11. What is the difference between the leading and lagging strands? 12. Describe the steps of DNA replication as shown in Figure 16.17, page 317. 13. What is ...
... 9. Make a list of the enzymes involved in replication and their roles. 10. Why does the DNA have to add nucleotides in the 5’ to 3’ direction? 11. What is the difference between the leading and lagging strands? 12. Describe the steps of DNA replication as shown in Figure 16.17, page 317. 13. What is ...
DNA ppt
... replicated exactly. • Each strand can be used to make the other strand. • Many enzymes are involved. ...
... replicated exactly. • Each strand can be used to make the other strand. • Many enzymes are involved. ...
Replisome
The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.