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Ch 1 - Composition of cells, DNA
Ch 1 - Composition of cells, DNA

... The order of the nitrogenous bases A, T, G & C is very important. The sequence provides cells with templates for the production of every protein in the body These proteins play many different roles in the body Each segment of DNA which determines the structure of one protein is called a gene ...
Human Mitochondrial DNA
Human Mitochondrial DNA

... Endonucleases – enzymes that cut RNA or DNA at specific sites; restriction enzymes are endonucleases that cut DNA Sticky cells – restriction fragments in which one end of the double stranded DNA is longer than the other; necessary for the formation of recombinant DNA Restriction enzyme mapping – det ...
Energy Unit SG Key
Energy Unit SG Key

Study Guide Chapter 16- Molecular basis of Inheritance
Study Guide Chapter 16- Molecular basis of Inheritance

... 3. Primase adds a short sequence of RNA nucleotides (called a primer). The primer is complementary to the DNA template strand. 4. DNA pol III adds nucleotides to 3’ end of the primer, synthesizing the new DNA strand in the 5’  3’ direction. The DNA Pol III synthesizes the new DNA strand away from t ...
Mader/Biology, 11/e – Chapter Outline
Mader/Biology, 11/e – Chapter Outline

... semiconservative, with each strand of the original double helix (parental molecule) serving as a template (mold or model) for a new strand in a daughter molecule. This process consists of: a. Unwinding: old strands of the parent DNA molecule are unwound as weak hydrogen bonds between the paired bas ...
I-Modified Nucleosides as DNA-Sugar Centered Radical Precursors
I-Modified Nucleosides as DNA-Sugar Centered Radical Precursors

... oxygen is reductively converted into superoxide as a by-product.[9] Through complex biochemical processes, superoxide may be converted into various reactive oxygen species (ROS), e.g. hydroxyl radicals (•OH), H2O2, 1O2, etc. These ROS and in particular the highly diffusible •OH are known to cause ch ...
DNA
DNA

... 4 Bases: Adenine (A), Guanine(G), Cytosine(C), Thymine(T) ...
Document
Document

... 14. What is the haploid number of chromosomes for a human sperm or egg cell? 15. What is the number of chromosomes in a body cell known as? 16. Who is responsible for discovering the structure of DNA (two names)? 17. _______________stands for deoxyribonucleic acid. 18. _______________stands for ribo ...
aps6-artifact - Clemson University
aps6-artifact - Clemson University

... o Replication proceeds in ______________ directions until each chromosome is completely copied. o The sites where separation and replication occur are called _______________ _________. ...
DNA Quantification: Comparison of UV
DNA Quantification: Comparison of UV

Who should get the Nobel prize Who are my all
Who should get the Nobel prize Who are my all

... that aphids arise from the dew which falls on plants, flies from putrid matter, mice from dirty hay, crocodiles from rotting logs at the bottom of bodies of water, and so on. After ca. 2000 years this nonsense idea went away! ...
Nucleic Acids and the RNA World
Nucleic Acids and the RNA World

Name: Date: Period:_____ Midterm Review: Study Guide # 3
Name: Date: Period:_____ Midterm Review: Study Guide # 3

... 2. Next, scan the objectives for the topic you are about to study in order to get a sense of what you should be focusing your time and energy on. 3. Start mastering each objective by answering the associated review questions right on this sheet. 4. After you have finished, use this sheet as a study ...
Analysis of DNA polymerase activity in vitro using non
Analysis of DNA polymerase activity in vitro using non

bp) and it does not contain any stop codons in the same frame as
bp) and it does not contain any stop codons in the same frame as

... DNA that otherwise would stall replicative polymerases. They differ from replicative polymerases in that they can tolerate large adducts on the bases (as they have much larger active sites that can accommodate damaged bases), they are much more error-prone (as they lack the 3' to 5' proofreading fun ...
Exam 2 review - Iowa State University
Exam 2 review - Iowa State University

... 37. A person’s STR may vary from another person’s STR because: A. The order of nucleotides B. The bases that are present C. The chromosomal location of the STR D. The number of times a sequence is repeated E. The number of coding regions 38. What is the function of the coding sequence? A. Determines ...
Deoxyribonucleic acid, or DNA, carries the hereditary information
Deoxyribonucleic acid, or DNA, carries the hereditary information

Lambda Vectors and their replication
Lambda Vectors and their replication

... • size of DNA to be introduced into the host cell • Problem: when making genomic libarary of large size (plants and mammals) only a portion of those fragments will be represented. If gene of interest is located in a large fragment, then you won’t be able to isolate that gene from the library. • Solu ...
Genetic Engineering
Genetic Engineering

... humans don't need more than 25,000 genes to function.B) the exons used to make a specific mRNA can be rearranged to form genes for new proteins.C) the sample size used to sequence the human genome was not big enough, so the number of genes estimated could be low.D) the estimate will increase as scie ...
Ch. 17 - Ltcconline.net
Ch. 17 - Ltcconline.net

... 15. Describe the process of translation (including initiation, elongation, and termination) and explain which enzymes, protein factors, and energy sources are needed for each stage. 16. Describe how a polypeptide must be modified before it becomes fully functional. 17. Compare protein synthesis in ...
In meiosis, what is the difference between metaphase 1 and
In meiosis, what is the difference between metaphase 1 and

... (first do square crossing mom’s grandparents to find out what mom is. Since grandparents are type A they could be either IAIA or IAi. We know the dad has to be IBi because they have a daughter with B in their blood and another with little”i”. We also know the mom has to be IAi instead of IAIA becaus ...
powerpoint
powerpoint

Transposons: Mobile DNA DNA
Transposons: Mobile DNA DNA

No Slide Title
No Slide Title

... • It means the tendency of a polymerase to stick with the replicating job once it starts. • When we said this polymerase is highly processive, meaning that once it starts replicating DNA, it won’t stop for a long ...
These essay/free response questions may be used on your various
These essay/free response questions may be used on your various

... * How are triglycerides and phospholipids similar? different? * How do the differences between triglycerides & phospholipids affect their metabolic functions? * How is cholesterol’s structure different from other membrane lipids? * What are glycoproteins? lipoproteins? * Illustrate the nature of a l ...
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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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