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Problem Set 1A
Problem Set 1A

... Legitimate recombination is recombination between two DNA sequences that share regions of high similarity, as opposed to illegitimate recombination, which is recombination between two DNA sequences which share very little sequence similarity. Legitimate recombination is the most common. 5. Most case ...
Planet Earth and Its Environment A 5000
Planet Earth and Its Environment A 5000

... is, the DNA unspirals, hydrogen bonds between the two strands break, and the strands separate over a short length, just in that part of the DNA that holds the gene to be used. Only one strand of DNA contains the genetic information to make a protein; rather confusingly, it is called the non-coding s ...
Document
Document

... • Use ______________________ ________________________ • Cuts DNA at specific sequences • DNA from one organism that is spliced into the DNA of another is called ________________________ DNA •Separating DNA –Use ____________________________________________________________ •Mix DNA fragments placed in ...
UNIT REVIEW_DNA to Protein Synthesis
UNIT REVIEW_DNA to Protein Synthesis

... 46. What is the purpose of translation? ____Translastion is the process of reading mRNA and converting it___ ____into a chain of amino acids that will form a protein_______ 47. Translation is the process of making _____amino acid chains/proteins___ from __the code on mRNA_______. 48. Where in a cell ...
BIOLOGY 210 FALL 2004
BIOLOGY 210 FALL 2004

... Course goals and requirements: This course is designed for students to gain a fundamental understanding of human genetics. Genetics is the study of inherited traits and their variation. We will explore all aspects of genetics, including DNA, genes, chromosomes, and genomes. We will examine genetics ...
Chapter 12: Biotechnology 1. Recombinant DNA What is
Chapter 12: Biotechnology 1. Recombinant DNA What is

... • the PCR (Polymerase Chain Reaction) technique is routinely used to generate huge numbers of identical DNA fragments • involves in vitro DNA replication to amplify desired DNA sequences ...
DNA * Deoxyribonucleic Acid
DNA * Deoxyribonucleic Acid

... and map the 3 billion nucleotide sequences of DNA in human chromosomes. Currently, scientists are using this information to identify where genes are located, control mechanisms of genes and the functions of genes. ...
Cell Division
Cell Division

inv3DNA - Empidonax-hommondii10-11
inv3DNA - Empidonax-hommondii10-11

... There are two kinds of genes, recessive and dominant. Recessive genes are the least likely to occur in a cell. Dominant are the most common genes and every living thing has at least one. There are actually diseases that occur when a being has too many recessive genes. For example some recessive gene ...
Chapter08_MBP1022H
Chapter08_MBP1022H

... DNA CLONING: PLASMIDS PLASMID: A circular double-stranded DNA molecule that replicates in bacteria and is separate from the bacterial genome • engineered to contain only sequences needed to function as a DNA cloning vector: • a bacterial origin of replication (ori) • an antibiotic resistance gene ( ...
Test File
Test File

... 8. A DNA recombination intermediate before its resolution into two recombined strands is called a(n) a. Holliday junction. b. RecBCD complex. c. cross-over complex. d. attachment site. 9. Which of the following statements is false about integrase, the enzyme that catalyzes the integration of bacteri ...
Prokaryotes
Prokaryotes

... Wider range of environments Greater diversity Single, circular chromosome Best known as bacteria ...
DNA extraction from cheek cells protocol I mailed to you
DNA extraction from cheek cells protocol I mailed to you

... proteins, the DNA will separate from the proteins and unwind. Each DNA molecule consists of two strands of nucleotides twisted together in a long spiral called a double helix. DNA is made up of four different types of nucleotide: A, C, G and T. Each DNA molecule contains multiple genes. Each gene is ...
Understanding DNA Technology - Southern Beef Technology Services
Understanding DNA Technology - Southern Beef Technology Services

Chemistry & Biology
Chemistry & Biology

The Transcription Process
The Transcription Process

CST Review Sheet 2 DNA and RNA 1. The unit to the right which
CST Review Sheet 2 DNA and RNA 1. The unit to the right which

... 6. Which of the following statements correctly describes meiosis? A Cells divide only once during meiosis. B Meiosis does not occur in reproductive cells. C The cells produced at the end of meiosis are genetically identical to the parent cell. D The cells produced at the end of meiosis contain half ...
Chapter 3
Chapter 3

... codon - sequence of three nucleotides in DNA or mRNA that specifies a particular amino acid during protein synthesis; also called triplet. Of the 64 possible codons, three are stop codons, which do not specify amino acids complementary DNA (cDNA) - DNA that is synthesized in the laboratory from a me ...
pathogens2
pathogens2

... Proteases block building of Virus Proteins Integrases block integration of vDNA into host DNA  AZT prematurely ends viral DNA replication  Natural Immunities (CD-4 altered or super ...
DNA SEQUENCING DNA sequencing
DNA SEQUENCING DNA sequencing

... Chain-termination methods The key principle of the Sanger method was the use of dideoxynucleotide triphosphates (ddNTPs) as DNA chain terminators. The classical chain-termination method requires a single-stranded DNA template, a DNA primer(labelled ), a DNA polymerase, normal deoxynucleotidetriphos ...
DNA - Madison Public Schools
DNA - Madison Public Schools

... A sugar molecule that forms the backbone A phosphate group which links the sugars A nitrogen containing base (adenine, cystine, ...
Measuring forces in the DNA molecule
Measuring forces in the DNA molecule

... properties of the molecular interactions, we are better able to work with these molecules." At the moment, the lab is building a molecular rotational motor out of DNA, the components of which interlock and are held together via stacking forces. The goal is to be able to control a directed rotation v ...
MB206_fhs_Int_005c_AT_Jan09
MB206_fhs_Int_005c_AT_Jan09

Genetic mechanisms
Genetic mechanisms

DNA - s3.amazonaws.com
DNA - s3.amazonaws.com

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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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