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DNA and Biomolecules - Warren County Schools
DNA and Biomolecules - Warren County Schools

... DNA Structure • DNA is composed of a sugar-phosphate backbone and ladder of nitrogenous base pairs. • Adenine pairs with thymine • Cytosine pairs with guanine • There are 46 chromosomes in the nucleus of most cells (except sex cells…they have 23). This is called nuclear DNA. • One chromosome pair i ...
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MYP unit planner

... (by a templating mechanism). Know that each DNA molecule in a cell is a single chromosome.  12.11.22: Understand that a gene is a set of instructions in the DNA sequence of each organism that specifies the sequence of amino acids in polypeptides characteristic of that organism.  12.11.23: Understa ...
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Novel DNA Polymerase Increases Efficiency of Multiple PCR

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Chem 317 Exam II
Chem 317 Exam II

... information is then translated to create proteins the cell can use. Transcription process of eukaryotic cells differs from prokaryotic cells in several ways. Conversion of heterogeneous RNAs (hnRNAs) of eukaryotic cells to mature mRNA requires several post-transcriptional modifications (Modification ...
Nucleic Acids
Nucleic Acids

... Secondary Structure: DNA Double Helix • In DNA there are two strands of nucleotides that wind together in a double helix - the strands run in opposite directions - the bases are arranged in step-like pairs - the base pairs are held together by hydrogen bonding • The pairing of the bases from the tw ...
Southern Blotting
Southern Blotting

... • Such variations include: ABO blood type, Rhesus factor, and major histocompatibility complex (MHC). • RFLP are differences in homologous DNA sequences that can be detected by the presence of fragments of different lengths after digestion of the DNA. • Moreover, RFLP is a sequence of DNA that has a ...
Telomeres - OpenWetWare
Telomeres - OpenWetWare

... identity between Rpa3 and Ten1, and therefore we cannot conclude whether Ten1 contains an OB-fold domain or not. This may be a reflection of the fact that both proteins have diverged rapidly at the primary sequence level, as revealed by the alignments of Rpa3 and Ten1 sequences from fungal genomes. ...
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PCR: Polymerase Chain Reaction

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L14 Gene to Protein Fa08

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DNAandGeneticsEducDept

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Lecture 20 DNA Repair and Genetic Recombination

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... according to instructions coded in DNA. Recall that proteins have many functions, including acting as enzymes and cell membrane channels. Proteins, however, are not built directly from DNA. Ribonucleic (rie boh noo KLAY ihk) acid is also involved. Like DNA, Ribonucleic acid (RNA) is a nucleic acid—a ...
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emboj7601266-sup

... cells, and insoluble proteins were removed by centrifugation (25 min at 3000 and 12000 rpm, respectively). DNA was then removed by polyethyleneimine (PEI, Sigma) precipitation (0.3% per 120 U A260). Next, the salt concentration was lowered to 0.1 M to precipitate proteins. The protein pellet was res ...
LE 01-10b - Biology at Mott
LE 01-10b - Biology at Mott

... Flight muscles obtain energy in a usuable form from organelles called mitochondria. Molecules in the highly folded inner membrane carry out many of the steps in energy production. ...
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Genetic engineering – stepping stones

... Cells are broken open and DNA is separated. Restriction enzymes cut DNA at specific points. Fragments of DNA are put into gel. Gel electrophoresis sorts fragments according to size. Radioactive probes help to make the pattern visible. ...
Exam Review 2 - Fullfrontalanatomy.com
Exam Review 2 - Fullfrontalanatomy.com

... E) bacteriophages 79) At one point, you were just an undifferentiated, single cell. You are now made of many cells; some of these cells function as liver cells, some as muscle cells, some as red blood cells, while others play different roles. What name is given to the process that is responsible for ...
Problem Set 1A
Problem Set 1A

... Legitimate recombination is recombination between two DNA sequences that share regions of high similarity, as opposed to illegitimate recombination, which is recombination between two DNA sequences which share very little sequence similarity. Legitimate recombination is the most common. 5. Most case ...
Transcription - Kenmore Tonawanda UFSD
Transcription - Kenmore Tonawanda UFSD

... GY ...
< 1 ... 309 310 311 312 313 314 315 316 317 ... 657 >

Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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