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PCR amplifies any target DNA sequence. (N)
PCR amplifies any target DNA sequence. (N)

... 8. “Southern” blotting detects sequences by hybridization. 9. Microarrays detect gene expression patterns over the genome. 10. Genes can be knocked out (deleted) or replaced in prokaryotes and eukaryotes. (N) ...
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M-MuLV Reverse Transcriptase, RNase H minus
M-MuLV Reverse Transcriptase, RNase H minus

Module 7: DNA, RNA, and Proteins - Peer
Module 7: DNA, RNA, and Proteins - Peer

... 6. transcription: manufacture of proteins using mRNA and tRNA; occurs on ribosomes in the rough endoplasmic reticulum; involves the encoding of a sequence of triplets into a comple-mentary sequence of codons. 7. translation: the raw materials for the process are free amino acids in the cytoplasm or ...
Day_1_-_DNA
Day_1_-_DNA

... clamps DnaB helicase protein as a hexamer to each strand in the open DNA ...
Immobilization and stretching of DNA molecules in a
Immobilization and stretching of DNA molecules in a

... A new technique can immobilize and stretch a large number of DNA molecules for single-molecule DNA analysis applications. DNA-protein interactions drive the cellular machinery for maintaining and transcribing DNA. To study the motion and kinetics of proteins along a DNA strand at the single-molecule ...
PPT NOTES_AP Biology Chapter 17 Notes
PPT NOTES_AP Biology Chapter 17 Notes

...  They seem to facilitate the ______________ of mRNA  They __________________ mRNA from hydrolytic enzymes  They help ___________________ attach to the 5 end • Most eukaryotic genes and their RNA transcripts have long noncoding stretches of nucleotides that lie between coding regions - called int ...
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... • Main purpose of RNA: – Protein synthesis! ...
DNA
DNA

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chapter_3_2007
chapter_3_2007

... Held together by hydrogen bonds between the nitrogenous bases. The bases pair according to base pair rules. ...
mg-lecure1 - WordPress.com
mg-lecure1 - WordPress.com

... • The double helix: DNA consists of two polynucleotide strands. The sugar–phosphate groups of each polynucleotide strand are on the outside of the molecule, and the bases are in the interior. • Hydrogen bonding joins the bases of the two strands: guanine pairs with cytosine, and adenine pairs with t ...
Ch. 13 Genetic Engineering
Ch. 13 Genetic Engineering

... embryonic cell (whose DNA has recombined with a human gene) is placed into an egg whose nucleus has been removed. The “new” egg is then placed into the uterus of an animal. ...
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DNA–DNA hybridisation

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Lab 11: DNA Testing

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4.4.1 Evidence to support the theory of evolution

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Mutations - The Super Heroes of Biology
Mutations - The Super Heroes of Biology

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... concise one page paper in the British Journal Nature. The paper reported their molecular model of DNA, the double helix, which has since become the symbol of molecular biology. The beauty of the model was that its structure suggested the basic mechanism of DNA replication. Watson and Crick suggested ...
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DNA - 長庚大學生物醫學系
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... because many scientists still believe that DNA is too simple a molecule to be the genetic material. • 1949 Erwin Chargaff, a biochemist, reports that DNA composition is speciesspecific; that is, that the amount of DNA and its nitrogenous bases varies from one species to another. In addition, Chargaf ...
C17: From Gene to Protein
C17: From Gene to Protein

... mRNA codon. The most versatile bases have an (inosine) I in the third position and can bind to U, C, or A. Aminoacyl-tRNA synthetase – enzyme that joins each aa to the correct tRNA (energized by ATP). Ribosomes are made of two subunits, large and small. The subunits are made of proteins and RNA mole ...
dna testing workshop 2005
dna testing workshop 2005

... information on the attached pages. Be sure to use conventional genetic symbols to symbolize marriages, individuals affected by the trait (cancer), males, females, children, unknown individuals, etc. Using the symbol T for the normal tumor suppressor gene and t for the mutant gene, and recalling that ...
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... In molecular terms, epigenetics is concerned with the study of chromatin organisation in the eukaryotic nucleus, with respect to the regulation of gene expression during the development of plants, fungi, insects and animals through embryogenesis to adulthood, and in disease states. A major intellect ...
Recombinant DNA Technology (b)
Recombinant DNA Technology (b)

... Recombinant DNA Technology Production of a unique DNA molecule by joining together two or more DNA fragments not normally associated with each other, which can replicate in the living cell. Recombinant DNA is also called Chimeric DNA Developed by Boyer and Cohen in 1973 3 different methods of D ...
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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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