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regulation of cell cycle
regulation of cell cycle

... to prepare the chromosomes for replication. Rising level of S-phase promoting factor (SPF) — which includes cyclin A bound to Cdk2 — enters the nucleus and prepares the cell to duplicate its DNA (and its centrosomes). As DNA replication continues, cyclin E is destroyed, and the level of mitotic cy ...
DNA Biology and Technology
DNA Biology and Technology

18 - cloudfront.net
18 - cloudfront.net

... Reading the Sequence Researchers use a clever chemical trick to "read" DNA by determining the order of its bases. A single strand of DNA whose sequence of bases is not known is placed in a test tube. DNA polymerase, the enzyme that copies DNA, and the four nucleotide bases, A, T, G, and C, are added ...
13-2 Manipulating DNA
13-2 Manipulating DNA

... Reading the Sequence Researchers use a clever chemical trick to "read" DNA by determining the order of its bases. A single strand of DNA whose sequence of bases is not known is placed in a test tube. DNA polymerase, the enzyme that copies DNA, and the four nucleotide bases, A, T, G, and C, are added ...
chapter 16 the molecule basis of inheritance
chapter 16 the molecule basis of inheritance

... Returning to the original problem at the replication fork, the leading strand requires the formation of only a single primer as the replication fork continues to separate. For synthesis of the lagging strand, each Okazaki fragment must be primed separately.  Another DNA polymerase, DNA polymerase I ...
Structural analysis of the protein complex involved in the
Structural analysis of the protein complex involved in the

Document
Document

... • Each strand can be used to make the other strand. • Many enzymes are involved. ...
Protein Synthesis - TangHua2012-2013
Protein Synthesis - TangHua2012-2013

... E. mRNA is released (Enzymes break the Hydrogen bonds). DNA ________________ back together. mRNA is first processed (_________________________________________________________) so it can leave the nucleus then passes through the _________________________ through the nuclear pores into the cytoplasm. ...
Chargaff`s Rules - Rutgers University
Chargaff`s Rules - Rutgers University

Lesson Plan Construction Form
Lesson Plan Construction Form

... 4. Fasten your molecule together using clear tape. Do not tape across base pairs. 5. As in step 1, copy the parts for A, G, and C RNA nucleotides. Use the same colors of construction paper as in step 1. Use the fifth color of construction paper to make copies of uracil nucleotides. 6. With scissors, ...
DNA_to_Protein
DNA_to_Protein

RNA Transcription
RNA Transcription

... strand for complementary base-pairing. In this way, the growing RNA chain is extended by one nucleotide at a time in the 5’-to-3’ direction, proceeding at a rate of about 50 nucleotides per second. The substrates are nucleoside triphosphates (ATP, CTP, UTP, and GTP), but unlike the situation in DNA ...
Practice using the RNA codon * amino acid Codon Chart*
Practice using the RNA codon * amino acid Codon Chart*

... Name _____________________________________________________________ Date: ________________ Period: __________ INTRODUCTION: Protein synthesis is the process used by the body to make proteins. The first step of protein synthesis is called Transcription. It occurs in the nucleus. During transcription, ...
Chapter 7: Nucleic Acids and Protein Synthesis
Chapter 7: Nucleic Acids and Protein Synthesis

Sunlight Water Entropy
Sunlight Water Entropy

1431236491.
1431236491.

... Heavy isotope was integrated into all the bacterial DNA (Grew E. coli in 15N for several generations so that all the DNA was labeled) They shifted cells to 14N media and allowed them to replicate their DNA 1 time Sample of DNA was taken. Cells were allowed to replicate their DNA again (total of 2 ti ...
Chapter 8: Microbial Genetics
Chapter 8: Microbial Genetics

... Each cell has a nucleus, each nucleus has chromosomes And DNA, baby, that spells DNA ...
Genetic Continuity
Genetic Continuity

... 3. unpaired bases react with complimentary bases of nucleotides 4. New H bonds are formed ◦ DNA polymerase (an enzyme) catalyzes the process ...
Nucleic Acids - Fort Bend ISD / Homepage
Nucleic Acids - Fort Bend ISD / Homepage

...  Basically a chromosome is made up of DNA wrapped around proteins, coiled together to form nucleosomes that coil up into supercoils called a CHROMOSOME. ...
Genetic Engineering Notes
Genetic Engineering Notes

... Dolly, the first mammal to be cloned from adult DNA, was put down by lethal injection Feb. 14, 2003. Prior to her death, Dolly had been suffering from lung cancer and crippling arthritis. Although most Finn Dorset sheep live to be 11 to 12 years of age, postmortem examination of Dolly seemed to indi ...
www.dna-‐sports-‐performance.com
www.dna-‐sports-‐performance.com

Chapter 8: Recombinant DNA Technology 1. Tools of Recombinant
Chapter 8: Recombinant DNA Technology 1. Tools of Recombinant

... known sizes (kilobase pairs) ...
dna and protein synthesis - YISS
dna and protein synthesis - YISS

Assignment 2 with Key
Assignment 2 with Key

... (b) Histones are rich in the electropositive (basic) amino acid residues lysine and arginine. These positive charges neutralize the negative charge of chromosomal DNA, which allows the strands to become condensed and packaged within the chromosome. ...
Cross-Curricular Discussion
Cross-Curricular Discussion

... 1. What are some of the important molecular and functional differences between DNA and RNA? [DNA and RNA have extremely similar molecular structures, but DNA is double-stranded while RNA is most often single-stranded. Instead of the base thymine, which is used in DNA, RNA has uracil which has a sli ...
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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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