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... the linear DNA phenotype, just like deletion of ruvABC does. Therefore: ruvC may be directly breaking the chromosome. But note that rep recBCTS ruvC is lethal while rep recBCTS ruvABC is fine. So ruvC is lethal only when ruvAB are active. ...
Biotechnology - drzapbiology
Biotechnology - drzapbiology

... to every time it appears in the text. • Draw a vertical line after the t. • The vertical line represents a cut site. • If you were to cut at that cut site with scissors, how many text fragments would you have? Write the number in the designated spot on the note-taker. ...
CHAPTER 17 - HCC Learning Web
CHAPTER 17 - HCC Learning Web

... • During the elongation stage, amino acids are added one by one to the preceding amino acid at the C-terminus of the growing chain • Each addition involves proteins called elongation factors and occurs in three steps: codon recognition, peptide bond formation, and translocation • Translation proceed ...
Some words to think about
Some words to think about

MCDB 1041 3/9/12 Activity 6: Central Dogma Continued PART I
MCDB 1041 3/9/12 Activity 6: Central Dogma Continued PART I

... PART I: Types of mutations: • Point mutations: changes in a single base of DNA. Silent mutations: change in sequence of DNA results in no change in the amino acid sequence. Missense mutations: change in DNA sequence results in a different amino acid placed into the protein. Nonsense mutations: chang ...
Bartlett`s Lecture
Bartlett`s Lecture

Review handout
Review handout

... - splicing to eliminate introns i. introns: segments of unknown function that do not code for polypeptides ii. exons: coding sequences iii. alternative splicing: the fact that the pre-mRNA transcript can be alternatively spliced in different cell types. -CTD coordinates the processing events ...
Protein Synthesis Simulation Activity
Protein Synthesis Simulation Activity

... produced OUTSIDE of the nucleus. So how does the cell solve this problem? It sends a “messenger” from the nucleus to the ribosomes in the cytoplasm. In a process called transcription, the DNA code is transcribed (copied) into mRNA, following rules similar to DNA replication we saw earlier (see below ...
Honors Genetics: Senior Exam Review Chapter 1: Introduction to
Honors Genetics: Senior Exam Review Chapter 1: Introduction to

... Chapter 10: DNA Replication and Recombination Why must DNA replicate? Describe the process of DNA replication as a semiconservative replication process. Understand the difference between conservative and dispersive replication. How did the Messelson-Stahl experiment prove semiconservative replicatio ...
1495/Chapter 07
1495/Chapter 07

... tightly bound and stable structure. Because the bases face into the interior of the molecule, the helix must be unwound for the individual chains of nucleotides to serve as templates for the formation of new strands. The points at which the DNA helix is unwound and new strands develop are called rep ...
Giant DNA Lab Manual.
Giant DNA Lab Manual.

... in this organism; that is, if you built the gene as you did in step (11) and followed steps (12) through (15), it would produce the protein desired. 1) Give the sequence of your protein. Be sure to indicate the N and C termini. 2) Give the sequence of the mRNA that would encode your protein. Note th ...
Gel Electrophoresis of DNA
Gel Electrophoresis of DNA

... What is Gel Electrophoresis? • Gel electrophoresis refers to the separation of charged particles located in a gel when an electric current is applied • Charged particles can include DNA, amino acids, peptides, etc ...
EOC Checklist
EOC Checklist

...  Proteins that DNA coils around in order to condense into chromosomes are called _____________.  I have reviewed DNA replication. Basically, o Helicase _______________ the DNA strands, which allows the nitrogen bases to be exposed. o Then, DNA polymerase moves along the strand, adding ___________ ...
Reading Packet 5- Molecular Genetics Part 1 Chapter 16
Reading Packet 5- Molecular Genetics Part 1 Chapter 16

... 19. What are telomeres? Why do they exist in eukaryotic chromosomes? Draw your own diagram explaining the problem. ...
http://www.councilforresponsiblegenetics.org/genewatch
http://www.councilforresponsiblegenetics.org/genewatch

Drosophila Melanogaster
Drosophila Melanogaster

... (2) Digest a restricton enzyme, e.g. EcoRI (3) Ligate the digested DNA ...
Bioreg2017_Replication1_V3
Bioreg2017_Replication1_V3

Protein Synthesis
Protein Synthesis

... Definition of Protein Synthesis • Making a protein by first transcribing (making a readable copy of) a gene and then translating its genetic code into an amino acid chain ...
Ch. 12 DNA - Fort Bend ISD
Ch. 12 DNA - Fort Bend ISD

DNA & RNA - Cobb Learning
DNA & RNA - Cobb Learning

... What are genetic mutations? Mutation: Permanent change in a cell’s DNA, ranging from changes in a single base pair to deletions of large sections of chromosomes. Causes of mutations include: ...
Diapositive 1 - ac-aix
Diapositive 1 - ac-aix

... material in all the alive beings? We wants to extract from the DNA of different cellular species such as: - Plant species : the onion - Animal species eukaryote* pluricellular : saliva - Animal species eukaryote* pluricellular : liver of mouse - Animal species prokaryote** : bacterium *: cell :conta ...
What is RNA? - Biology for Life
What is RNA? - Biology for Life

... • RNA is essential for all known forms of life. • Like DNA, RNA is made up of a long chain of nucleotides. Each consists of a base, a ribose sugar, and a phosphate group. ...
Exp.9 Bacterial Transformation
Exp.9 Bacterial Transformation

DNA Extraction from Strawberry - Partnership for Biotechnology and
DNA Extraction from Strawberry - Partnership for Biotechnology and

... Plant cell membrane- phospholipid bilayer ...
Exp.9 Bacterial Transformation
Exp.9 Bacterial Transformation

...  Occurs in nature, but rarely  If the foreign DNA has an origin of replication recognized by the host cell DNA polymerases, the bacteria will replicate the foreign DNA along with their own DNA. ...
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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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