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Eukaryotic DNA Replication
Eukaryotic DNA Replication

... Highly conserved throughout eukaryotes; archaea also possess an Mcm2-7 homologue ...
Cells
Cells

... Two nucleotide chains serve as templates for the formation of a new strand of nucleotides. Unattached nucleotides pair with the appropriate complementary nucleotide ...
DNA Technology and Genomes
DNA Technology and Genomes

... organisms or a group of cells derived from a single parent cell. Plasmid: circular DNA found in bacteria, not part of the nucleoid region Restriction Enzymes: enzymes that protect bacteria by cutting up foreign DNA from invaders DNA ligase: enzyme used to seal DNA strands together ...
Transcription and Translation
Transcription and Translation

... The instructions for protein structure are carried in the genes, which are sequences of DNA nucleotides. Three nucleotides code for an amino acid, e.g. AAA on the transcribing strand codes for phenylalanine whilst AAT codes for leucine. So, successive triplets of DNA nucleotides determine the sequen ...
Protein Synthesis
Protein Synthesis

... DNA contains the information that a cell needs to carry out all of its functions. In a way, DNA is like the cell’s encyclopedia. Suppose that you go to the library to do research for a science project. You find the information in an encyclopedia. You go to the desk to sign out the book, but the libr ...
DNA Replication Lab
DNA Replication Lab

From Gene to Protein—Transcription and Translation
From Gene to Protein—Transcription and Translation

... the figure on page 4 of your biology background and instructions handout.) Student answers may vary. Student should included: the idea that during protein synthesis the DNA “language” has to be converted in RNA “language” in order for the information it contains to be understood and carried out. Thi ...
The Genetics Revolution in the Life Sciences
The Genetics Revolution in the Life Sciences

... chapter and their relation to concepts in previous chapters. Each problem set begins with some problems based on the figures in the chapter, which embody important concepts. These are followed by problems of a more general nature. WORKING WITH THE FIGURES ...
Anticancer Drugs
Anticancer Drugs

Slide 1
Slide 1

... • RNA is the same as DNA except that the sugars in RNA have an extra oxygen and T is replaced by another pyrimidine called ...
Manipulating DNA - tools and techniques 2012
Manipulating DNA - tools and techniques 2012

...  Defined as the take up of naked DNA by cells  Occurs naturally in bacteria, yeast and some plants ...
Lecture 3
Lecture 3

... described the introduction of DNA into tobacco protoplasts but no transgenic plant was obtained. Subsequent reports described generation of transgenic tobacco and wheat but insufficient molecular evidence was provided. It appears that this method may be effective in introducing DNA into cell but not ...
video slide - Biology at Mott
video slide - Biology at Mott

... In 1953, James Watson and Francis Crick introduced an elegant double-helical model for the structure of deoxyribonucleic acid, or DNA DNA, the substance of inheritance, is the most celebrated molecule of our time Hereditary information is encoded in DNA and reproduced in all cells of the body This D ...
Lesson Title: Asthma and Genes
Lesson Title: Asthma and Genes

... Introduction: DNA fingerprinting is a fairly new technique used for identification in many species, particularly in humans in forensics. It can be used for paternity testing as well. This analysis uses DNA from a tiny amount of tissue such as skin, blood, or hair follicles. Certain sections of the D ...
BIOTECHNOLOGY - Bishop Amat Memorial High School
BIOTECHNOLOGY - Bishop Amat Memorial High School

... from bacteria (ex: EcoRI) and inserted into DNA of organism of choice! B. Restriction Enzyme specifically cuts DNA of selected organism at specific recognition sequences of nucleotides. ...
Genetic Diseases
Genetic Diseases

... more than females because they only have on X chromosome ...
Mutations WS
Mutations WS

... Mutation that enhance a protein’s ability to perform a function, or a mutation that produces a protein that perform a new function are beneficial mutations. Example: Lactose tolerance is a characteristic that gives humans with the ‘mutation’ the ability to produce an enzyme that breaks down sugars i ...
Nucleotides and Nucleic Acids
Nucleotides and Nucleic Acids

... 5hydroxyl group of the next nucleotide in the chain. These bridges are called phosphodiesters. Even though two of the four oxygen attached to the phosphorus of each bridge are tied up as phosphate esters, and one is present in phosphorus—oxygen double bond, one oxygen is free to lose a proton. It i ...
CH 13
CH 13

... from DNA to the ribosomes to make proteins •rRNA (ribosomal RNA): part of ribosomes •tRNA (transfer RNA): brings amino acids to the ribosomes ...
It`s in the genes – data storage turns to DNA
It`s in the genes – data storage turns to DNA

2001_Lewontin_In the Beginning Was the Word
2001_Lewontin_In the Beginning Was the Word

Bacterial Transformation of pGLO
Bacterial Transformation of pGLO

... • E. coli bacteria produce three enzymes (proteins) needed to digest arabinose as a food source. The genes which code for these enzymes are not expressed when arabinose is absent, but they are expressed when arabinose is present in their environment. How is this so? ...
Study Questions 2
Study Questions 2

Population Genetics
Population Genetics

... nm apart •Each chain makes one complete turn every 3.4 nm •So there are 10 bases per turn of the double helix ...
CHAPTER 11
CHAPTER 11

... are slightly different with regard to the number of base pairs per turn. Another difference is the RNA base pairing involved A to U, where DNA base pairing involves A to T. C17. Answer: The sequence in part A would be more difficult to separate because it has a higher percentage of GC base pairs com ...
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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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