Practical molecular biology
... DNA is polar and therefore insoluble in organic solvents. Traditionally, phenol:chloroform is used to extract DNA. When phenol is mixed with the cell lysate, two phases form. DNA partitions to the (upper) aqueous phase, denatured proteins partition to the (lower) organic phase. DNA is a polar molecu ...
... DNA is polar and therefore insoluble in organic solvents. Traditionally, phenol:chloroform is used to extract DNA. When phenol is mixed with the cell lysate, two phases form. DNA partitions to the (upper) aqueous phase, denatured proteins partition to the (lower) organic phase. DNA is a polar molecu ...
Chapter 4 Section 4 – The DNA Connection
... • The sugar molecule found in RNA is different from DNA. Ribose verse ...
... • The sugar molecule found in RNA is different from DNA. Ribose verse ...
PowerPoint Slides
... The Helios Gene Gun is a new way for in vivo transformation of cells or organisms (i.e. gene therapy and genetic immunization (DNA vaccination)). This gun uses Biolistic ® particle bombardment where DNA- or RNA-coated gold particles are loaded into the gun and you pull the trigger. A low pressure he ...
... The Helios Gene Gun is a new way for in vivo transformation of cells or organisms (i.e. gene therapy and genetic immunization (DNA vaccination)). This gun uses Biolistic ® particle bombardment where DNA- or RNA-coated gold particles are loaded into the gun and you pull the trigger. A low pressure he ...
The replication of DNA
... placement of sliding camp on DNA. These enzyme couple ATP binding and hydrolysis to the placement of sliding clamp around primer template junction, every time that this junction is present in the cell. The clamp loaders also remove the slide clamp from DNA once all of the enzymes that interact with ...
... placement of sliding camp on DNA. These enzyme couple ATP binding and hydrolysis to the placement of sliding clamp around primer template junction, every time that this junction is present in the cell. The clamp loaders also remove the slide clamp from DNA once all of the enzymes that interact with ...
Recombinant Biotechnology
... • Some bacteria and viruses insert their DNA into other cells that are to act as hosts • Bacteria use restriction enzymes to cut up foreign DNA • These characteristics are useful in recombinant technology ...
... • Some bacteria and viruses insert their DNA into other cells that are to act as hosts • Bacteria use restriction enzymes to cut up foreign DNA • These characteristics are useful in recombinant technology ...
Xeroderma Pigmentosum (XP)
... • Multiple forms of DNA damage occur • These are repaired constantly by several mechanisms • Failure to repair damage leads to mutations • Often defects in damage sensing machinery or DNA repair processes can be correlated with increased incidence of diseases such as cancer ...
... • Multiple forms of DNA damage occur • These are repaired constantly by several mechanisms • Failure to repair damage leads to mutations • Often defects in damage sensing machinery or DNA repair processes can be correlated with increased incidence of diseases such as cancer ...
Forensic DNA Testing Terminology ABI 310 Genetic Analyzer – a
... DNA polymerase – an enzyme that catalyzes the synthesis of double stranded DNA. DNA sequence – the relative order of base pairs, whether in a fragment of DNA, a gene, a chromosome, or an entire genome. Double Helix – the shape that two linear strands of DNA assume when bonded together. Dye blobs – a ...
... DNA polymerase – an enzyme that catalyzes the synthesis of double stranded DNA. DNA sequence – the relative order of base pairs, whether in a fragment of DNA, a gene, a chromosome, or an entire genome. Double Helix – the shape that two linear strands of DNA assume when bonded together. Dye blobs – a ...
Unit 2.1.2a - DNA (Deoxyribonucleic acid)
... produce different types of proteins. A DNA molecule consists of two chains of nucleotides, which run anti-parallel to each other: the two chains are joined by hydrogen bonds between the bases. This forms a double helix (same shape as a twisted ladder). DNA double helix: ...
... produce different types of proteins. A DNA molecule consists of two chains of nucleotides, which run anti-parallel to each other: the two chains are joined by hydrogen bonds between the bases. This forms a double helix (same shape as a twisted ladder). DNA double helix: ...
DNA
... 1. DNA is located in the nucleus. 2. DNA replication occurs in the nucleus. 3. DNA replication occurs during the S phase of Interphase in mitosis/meiosis. 4. DNA needs to replicate in order to assure that each cell has a complete set of identical genetic information. 5. Steps to DNA replication are ...
... 1. DNA is located in the nucleus. 2. DNA replication occurs in the nucleus. 3. DNA replication occurs during the S phase of Interphase in mitosis/meiosis. 4. DNA needs to replicate in order to assure that each cell has a complete set of identical genetic information. 5. Steps to DNA replication are ...
Name __________________________________ Period _________________
... 6. _______________________ Cell grows, more mitochondria are produced 7. _______________________ The cytoplasm divides and two new cells are formed. 8. _______________________ Centromere splits, chromatids separate and begin to move apart 9. _______________________ Spindle begins to form 10. _______ ...
... 6. _______________________ Cell grows, more mitochondria are produced 7. _______________________ The cytoplasm divides and two new cells are formed. 8. _______________________ Centromere splits, chromatids separate and begin to move apart 9. _______________________ Spindle begins to form 10. _______ ...
protein synthesis notes
... Organisms traits are determined by proteins Proteins are assembled according to genes on DNA DNA can not leave the nucleus, but proteins are made in ribosomes, therefore need an intermediate messenger… RNA 2 stages: Transcription – copying DNA info to mRNA (nucleus) Translation – mRNA us ...
... Organisms traits are determined by proteins Proteins are assembled according to genes on DNA DNA can not leave the nucleus, but proteins are made in ribosomes, therefore need an intermediate messenger… RNA 2 stages: Transcription – copying DNA info to mRNA (nucleus) Translation – mRNA us ...
Activity 3: What can we learn about human evolution from anatomy
... chimps, and gorillas by making model DNA molecules for each species and then comparing their similarities. The model DNA molecules are based on actual portions of the genes for hemoglobin proteins in these species. Procedure: Each color paper clip represents one of the 4 nucleotides found in DNA. Re ...
... chimps, and gorillas by making model DNA molecules for each species and then comparing their similarities. The model DNA molecules are based on actual portions of the genes for hemoglobin proteins in these species. Procedure: Each color paper clip represents one of the 4 nucleotides found in DNA. Re ...
CH 14 EXTRA CREDIT Study Guide
... 8. In order to get PKU, what must the parents be? 9. List all the genotypes and phenotypes of blood, not counting Rh. 10. In Huntington’s disease, the person usually is Hh but sometimes HH. What % of children will inherit Huntington’s if one parent has it? 11. What causes sickle-cell? 12. What cause ...
... 8. In order to get PKU, what must the parents be? 9. List all the genotypes and phenotypes of blood, not counting Rh. 10. In Huntington’s disease, the person usually is Hh but sometimes HH. What % of children will inherit Huntington’s if one parent has it? 11. What causes sickle-cell? 12. What cause ...
Chapter 6 and 9 - Wando High School
... 12. Describe what happens during Interphase. Draw how a cell may appear during this phase. DNA is replicated Chromosomes are not yet visible Proteins and RNA are synthesized Cell is preparing for Meiosis 13. Is there an Interphase between Meiosis I and Meiosis II? No 14. Describe crossing over and w ...
... 12. Describe what happens during Interphase. Draw how a cell may appear during this phase. DNA is replicated Chromosomes are not yet visible Proteins and RNA are synthesized Cell is preparing for Meiosis 13. Is there an Interphase between Meiosis I and Meiosis II? No 14. Describe crossing over and w ...
chapt17_lecture_anim_ppt
... • Fundamentally new method for DNA sequencing – DNA is cleaved into smaller pieces – Both ends are ligated to adapters that are complementary to specific primers – DNA fragments are injected into a flow cell – Each of 7 channels contains a solid substrate with primers that complement the ligated en ...
... • Fundamentally new method for DNA sequencing – DNA is cleaved into smaller pieces – Both ends are ligated to adapters that are complementary to specific primers – DNA fragments are injected into a flow cell – Each of 7 channels contains a solid substrate with primers that complement the ligated en ...
Epigenetics of Coeliac Disease
... • Epigenetics is the first level of integration of genetic and environmental factors. • It may translate the effects of risk factors in terms of molecular events. • It is feasable with the recent development of micro arrays/Si RNA knowledge. • There is no data published to date on this topic (pubmed ...
... • Epigenetics is the first level of integration of genetic and environmental factors. • It may translate the effects of risk factors in terms of molecular events. • It is feasable with the recent development of micro arrays/Si RNA knowledge. • There is no data published to date on this topic (pubmed ...
2nd semester exam Review packet
... flowers. This is an example of __________________________________. 55. In guinea pigs, smooth coat is dominant over rough coat and black coat is dominant over white coat. Complete the following cross SsBb x ssbb. Give the phenotype ratio. ...
... flowers. This is an example of __________________________________. 55. In guinea pigs, smooth coat is dominant over rough coat and black coat is dominant over white coat. Complete the following cross SsBb x ssbb. Give the phenotype ratio. ...
Chapter 10 (Sample questions)
... A charged tRNA enters the protein-synthesizing machinery at the ribosomes by recognition of its a. codon. b. anticodon. c. wobble. d. amino acid. e. none of the above. What is the minimum number of tRNA molecules required to produce a 60-amino acid polypeptide made up of only ten different kinds of ...
... A charged tRNA enters the protein-synthesizing machinery at the ribosomes by recognition of its a. codon. b. anticodon. c. wobble. d. amino acid. e. none of the above. What is the minimum number of tRNA molecules required to produce a 60-amino acid polypeptide made up of only ten different kinds of ...
General Biology (BIO 10)
... Reactants & products of cellular respiration (equation) Know the parts of cellular respiration (glycolysis, Krebs cycle, electron transport chain) Where does each occur? Function of cellular respiration When is oxygen necessary? Electron carrier molecules (like NAD+ and FADH+) How many ATP from Glyc ...
... Reactants & products of cellular respiration (equation) Know the parts of cellular respiration (glycolysis, Krebs cycle, electron transport chain) Where does each occur? Function of cellular respiration When is oxygen necessary? Electron carrier molecules (like NAD+ and FADH+) How many ATP from Glyc ...
DNA Extraction from Wheat Germ 01/29/13 I. Watch the following
... kit) into the jar with the bottom end in the tube touching the side or bottom of the tube. Slowly spin the glass rod in one direction (clockwise) so that the DNA winds around the rod like thread on a spool. If may help to tilt the tube at a 45 degree angle. The DNA should adhere to the wooden applic ...
... kit) into the jar with the bottom end in the tube touching the side or bottom of the tube. Slowly spin the glass rod in one direction (clockwise) so that the DNA winds around the rod like thread on a spool. If may help to tilt the tube at a 45 degree angle. The DNA should adhere to the wooden applic ...
Transcription 12.06.22A lec
... means automatically thymine is on the opposite side. Just in the structures of the molecules themselves, they have exactly the right shape, along with those different phosphate and sugar groups ...
... means automatically thymine is on the opposite side. Just in the structures of the molecules themselves, they have exactly the right shape, along with those different phosphate and sugar groups ...
Replisome
The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.