Unit 6 Guided Reading Questions
... _____ 20. How are transposable elements thought to have contributed to the evolution of the genome? A. Transposable elements that insert within regulatory sequences can affect protein production. B. During transposition, transposable elements can carry a gene or group to a new position in the genome ...
... _____ 20. How are transposable elements thought to have contributed to the evolution of the genome? A. Transposable elements that insert within regulatory sequences can affect protein production. B. During transposition, transposable elements can carry a gene or group to a new position in the genome ...
Genes get around
... Plasmids Are small, circular pieces of DNA in bacterial or yeast cells that contain 3 to 300 genes. Most plasmids exist separate from the chromosome of the cell. Usually replicated when DNA is copied, but some can reproduce at other times – autonomous replication ...
... Plasmids Are small, circular pieces of DNA in bacterial or yeast cells that contain 3 to 300 genes. Most plasmids exist separate from the chromosome of the cell. Usually replicated when DNA is copied, but some can reproduce at other times – autonomous replication ...
printer-friendly version
... groups called purines (A & G) and pyrimidines (T & C). The Human Genome Project has confirmed that the DNA in a typical human cell contains over 3 billion base pairs (bp). In these 3 billion bp are 20,000 to 25,000 genes that code for proteins, which in turn code for our traits. Each gene is a speci ...
... groups called purines (A & G) and pyrimidines (T & C). The Human Genome Project has confirmed that the DNA in a typical human cell contains over 3 billion base pairs (bp). In these 3 billion bp are 20,000 to 25,000 genes that code for proteins, which in turn code for our traits. Each gene is a speci ...
Document
... • denature the two strands • Incubation occurs next at 60ºC • DNA polymerase such as Taq polymerase • ample supply of nucleotides (dNTPs) • two complementary primers • short chains of nucleotides that match up using complementarity with a very small segment of the DNA fragment • defines the starting ...
... • denature the two strands • Incubation occurs next at 60ºC • DNA polymerase such as Taq polymerase • ample supply of nucleotides (dNTPs) • two complementary primers • short chains of nucleotides that match up using complementarity with a very small segment of the DNA fragment • defines the starting ...
Chapter 11: DNA and Genes
... How DNA Replicates During replication each strand serves as a pattern to make a new DNA molecule The end result is the formation of two DNA molecules that are identical (duplicated chromosome) Steps of Replication 1. Enzyme, DNA helicase, breaks the hydrogen bonds between nucleotides, this “un ...
... How DNA Replicates During replication each strand serves as a pattern to make a new DNA molecule The end result is the formation of two DNA molecules that are identical (duplicated chromosome) Steps of Replication 1. Enzyme, DNA helicase, breaks the hydrogen bonds between nucleotides, this “un ...
Linkage map - Cloudfront.net
... • Electricity is sent through gel containing DNA fragments • DNA pieces will migrate toward bottom o Smaller pieces will “run” faster o Larger pieces will be stuck toward top ...
... • Electricity is sent through gel containing DNA fragments • DNA pieces will migrate toward bottom o Smaller pieces will “run” faster o Larger pieces will be stuck toward top ...
Annex A: Highlights of the “Biotechnology Revolution”: 1953–present 1953 Nature
... Studies by David Botstein and others found that when a restrictive enzyme is applied to DNA from different individuals, the resulting sets of fragments sometimes differ markedly from one person to the next. Such variations in DNA are called restriction fragment length polymorphisms, or RFLPs, and th ...
... Studies by David Botstein and others found that when a restrictive enzyme is applied to DNA from different individuals, the resulting sets of fragments sometimes differ markedly from one person to the next. Such variations in DNA are called restriction fragment length polymorphisms, or RFLPs, and th ...
Lab/Activity: Prot
... will be used as the template to build mRNA, one base at a time. So first design an RNA polymerase enzyme to do this mRNA synthesis job. 3. You have also been supplied with mRNA nucleotides. Build a mRNA molecule, one base at a time, from this gene by transcribing your DNA template. Don’t forget to o ...
... will be used as the template to build mRNA, one base at a time. So first design an RNA polymerase enzyme to do this mRNA synthesis job. 3. You have also been supplied with mRNA nucleotides. Build a mRNA molecule, one base at a time, from this gene by transcribing your DNA template. Don’t forget to o ...
Techniques in Molecular Biology Cloning
... Design four PCR primers with overlap between insert/insert or insert/vector Adjacent segments should have identical sequences (A and B) PCR primers should contain 5’ end that is the same as adjacent sequence and contains the 3’ complementary ends ...
... Design four PCR primers with overlap between insert/insert or insert/vector Adjacent segments should have identical sequences (A and B) PCR primers should contain 5’ end that is the same as adjacent sequence and contains the 3’ complementary ends ...
Assignment 2
... Answer: c – will remain unaffected The disease results from a mutation of on the gene A and not B. Hence II-3 which does not contain the allele for A gene is normal Q4: What two enzymes are needed to produce recombinant DNA? a. endonuclease, transcriptase b. DNA polymerase, topoisomerase c. transcri ...
... Answer: c – will remain unaffected The disease results from a mutation of on the gene A and not B. Hence II-3 which does not contain the allele for A gene is normal Q4: What two enzymes are needed to produce recombinant DNA? a. endonuclease, transcriptase b. DNA polymerase, topoisomerase c. transcri ...
Inquiry into Life Twelfth Edition
... 6.3 Transcription Initiation • Transcription initiation was assumed to end as RNA polymerase formed 1st phosphodiester bond • Carpousis and Gralla found that very small oligonucleotides (2-6 nt long) are made without RNA polymerase leaving the DNA • Abortive transcripts such as these have been foun ...
... 6.3 Transcription Initiation • Transcription initiation was assumed to end as RNA polymerase formed 1st phosphodiester bond • Carpousis and Gralla found that very small oligonucleotides (2-6 nt long) are made without RNA polymerase leaving the DNA • Abortive transcripts such as these have been foun ...
DNA Replication, Transcription, Translation: What is the Difference?
... nitrogenous bases are adenine, thymine, cytosine and guanine. The two strands of DNA are connected through the weak hydrogen bonding of the nitrogenous bases. The base pairing rules are that adenine always pairs with thymine while cytosine always pairs with guanine (Campbell and Reece, 2005). These ...
... nitrogenous bases are adenine, thymine, cytosine and guanine. The two strands of DNA are connected through the weak hydrogen bonding of the nitrogenous bases. The base pairing rules are that adenine always pairs with thymine while cytosine always pairs with guanine (Campbell and Reece, 2005). These ...
Biology 340 Molecular Biology
... --Different cells express distinct subsets of genes. --Gene expression is regulated so genes are turned on when they are needed during development and in the correct cell types. --Most genes in higher eukaryotes are regulated by controlling their transcription. General principles: 1. Transcription b ...
... --Different cells express distinct subsets of genes. --Gene expression is regulated so genes are turned on when they are needed during development and in the correct cell types. --Most genes in higher eukaryotes are regulated by controlling their transcription. General principles: 1. Transcription b ...
notes
... Apply the cDNA mixture to a microarray, a microscope slide on which copies of singlestranded DNA fragments from the organism’s genes are fixed, a different gene in each spot. The cDNA hybridizes with any complementary DNA on the microarray. Rinse off excess cDNA; scan microarray for fluorescent. ...
... Apply the cDNA mixture to a microarray, a microscope slide on which copies of singlestranded DNA fragments from the organism’s genes are fixed, a different gene in each spot. The cDNA hybridizes with any complementary DNA on the microarray. Rinse off excess cDNA; scan microarray for fluorescent. ...
DNA Technology
... to change the information it contains. By changing this information, genetic engineering changes the type or amount of proteins an organism is capable of producing, thus enabling it to make new substances or perform new functions. ...
... to change the information it contains. By changing this information, genetic engineering changes the type or amount of proteins an organism is capable of producing, thus enabling it to make new substances or perform new functions. ...
Genomes 1
... The basics of genetic engineering are quite simple. The required gene is taken from one organism and inserted into the DNA/chromosome of another to produce recombinant DNA. To do this you need: ...
... The basics of genetic engineering are quite simple. The required gene is taken from one organism and inserted into the DNA/chromosome of another to produce recombinant DNA. To do this you need: ...
Document
... 35. Cells must regulate gene expression so that genes will be ____________________ only when the proteins are needed. 36. A cluster of genes in a bacterial cell that codes for proteins with related functions is called a(n) ____________________. 37. A protein that prevents transcription by blocking t ...
... 35. Cells must regulate gene expression so that genes will be ____________________ only when the proteins are needed. 36. A cluster of genes in a bacterial cell that codes for proteins with related functions is called a(n) ____________________. 37. A protein that prevents transcription by blocking t ...
From RNA to protein
... When DNA base sequences are cited, by convention it is the sequence of the non-template (sense, coding) strand that is given, even though the RNA is transcribed from the template strand. The following Table shows highlighted sequences from the HvCBF2A gene and their interpretation. ...
... When DNA base sequences are cited, by convention it is the sequence of the non-template (sense, coding) strand that is given, even though the RNA is transcribed from the template strand. The following Table shows highlighted sequences from the HvCBF2A gene and their interpretation. ...
Chapter 17 Presentation
... Termination occurs when a stop codon on the mRNA reaches the “A-site” within the ribosome. Release factor then binds to the stop codon in the “A-site” causing the addition of water to the peptide instead of an aa. This signals the end of translation. ...
... Termination occurs when a stop codon on the mRNA reaches the “A-site” within the ribosome. Release factor then binds to the stop codon in the “A-site” causing the addition of water to the peptide instead of an aa. This signals the end of translation. ...
Replisome
The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.