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Transcription and Translation Title: The Central Dogma: By Humans
Transcription and Translation Title: The Central Dogma: By Humans

... Direct the students representing mRNA to take their mRNA code through the nuclear membrane and out of the nucleus (the DNA and RNA polymerase cannot follow). Assign additional students to represent ribosomes. These students should be equipped with codon tables so that they can translate the mRNA int ...
DNA Structure Worksheet
DNA Structure Worksheet

... There are always _____ possible genotypes that produce the dominant phenotype. To have the dominant phenotype, you can be either ___________________ ________________ or _____________________. There is only _____ possible genotype that produces the recessive phenotype. To have the recessive phenotype ...
Introduction - Cedar Crest College
Introduction - Cedar Crest College

... Many eukaryotic chromosomes have repetitive sequences called telomeres at their ends that shorten after each round of cell division. These repeats bind to special proteins that maintain the stability of the chromosome ends. ...
USS Bio Snorks
USS Bio Snorks

... 5. How did you perform translation in this activity? ...
Transcription in prokaryotes Elongation and termination
Transcription in prokaryotes Elongation and termination

... Experiments with RNA-DNA hybrids •The RNA-DNA hybrids within the elongation complexes extend from position –1 to –9,-8 with respect to the 3’end of the emerging RNA. •The processivity of transcription depends upon the DNARNA hybrid at least 9bp long. ...
DNA Structure Worksheet
DNA Structure Worksheet

... There are always _____ possible genotypes that produce the dominant phenotype. To have the dominant phenotype, you can be either ___________________ ________________ or _____________________. There is only _____ possible genotype that produces the recessive phenotype. To have the recessive phenotype ...
Monohybrid Crosses
Monohybrid Crosses

... the side with the free OH group on the end -one strand is the 5’ (5 prime)= the side with the free phosphate on the end DNA Replication: The process by which a strand of DNA is copied occurs during something called replication. In order to do this, the enzyme DNA helicase moves down a molecule of DN ...
ORLANDO BIOLOGY ~ LESSON PLANS Competencies for 21st
ORLANDO BIOLOGY ~ LESSON PLANS Competencies for 21st

...  That the information passed from parents to offspring is coded in DNA molecules.  That DNA replication is semiconservative  That the processes of transcription and translation use the information from the DNA to make specific proteins.  That control of transcription is on the gene level and is ...
Self-Assembly at nano-Scale Binary Nanoparticles Superlattices
Self-Assembly at nano-Scale Binary Nanoparticles Superlattices

... with top-down lithography to fabricate complex devices is presently a major goal in nanoscience and technology. • Bridging the gap between self-assembly techniques and modern top-down lithography offers a way to incorporate additional functionality (for example, in the form of chemical or biological ...
File - Gander biology
File - Gander biology

... 1. Transcription- a strand of DNA is used as a template to make a strand of RNA, a single stranded nucleic acid (in the nucleus of the cell) 2. Translation-the order of the nucleotides in RNA is decoded in a sequence of amino ...
Restriction Fragment Length Polymorphisms
Restriction Fragment Length Polymorphisms

... different DNA sequences and therefore produce different fragments when a particular restriction enzyme is used to cut an equivalent section of DNA. •The fragments produced are called RFLPs, ...
Alief ISD Biology STAAR EOC Review Reporting Category 2
Alief ISD Biology STAAR EOC Review Reporting Category 2

... 1. Transcription- a strand of DNA is used as a template to make a strand of RNA, a single stranded nucleic acid (in the nucleus of the cell) 2. Translation-the order of the nucleotides in RNA is decoded in a sequence of amino ...
Chapter 9 – DNA-Based Information Technologies
Chapter 9 – DNA-Based Information Technologies

... specific recognition sequence • Type II - cleave double-stranded DNA only, at or near an unmethylated recognition sequence • More than 200 type I and type II are known • Most recognize “palindromic sequences” (read the same in either direction) ...
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... 1. Modify DNA Gene for Protein Expression DNA cloning for protein expression requires modification of the gene using the nucleotide code for guidance. A. Modify the gene of interest so that it includes recognition sequences in the gene for restriction enzymes that would enable the gene to be insert ...
Biology 2
Biology 2

... change of a single nucleotide in the coding strand of DNA. We now know that the alternative alleles of many genes result from changes in single base pairs in DNA. Any change in the nucleotide sequence of DNA is called a mutation. It can involve large regions of a chromosome or just a single nucleoti ...
Monohybrid Crosses
Monohybrid Crosses

... the side with the free OH group on the end -one strand is the 5’ (5 prime)= the side with the free phosphate on the end DNA Replication: The process by which a strand of DNA is copied occurs during something called replication. In order to do this, the enzyme DNA helicase moves down a molecule of DN ...
Genetics and Recombinant DNA
Genetics and Recombinant DNA

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Introduction to Genetical

DNA Technology - wvhs.wlwv.k12.or.us
DNA Technology - wvhs.wlwv.k12.or.us

Chapter 3 Protein Synthesis
Chapter 3 Protein Synthesis

... rRNA – Ribisomal RNA: found in the ribosomes it makes up part of the ribosome structure mRNA – messenger RNA: long single strand molecule, made in the nucleus during transcription, it travels to the ribosome and provides a code to manufacture proteins tRNA – transfer RNA: cross shaped molecule carry ...
SMRT Sequencing of DNA and RNA Samples Extracted
SMRT Sequencing of DNA and RNA Samples Extracted

... Molecule, Real-Time (SMRT) Sequencing offers a unique advantage in that it allows direct analysis of FFPE samples without amplification. However, obtaining ample long-read information from FFPE samples has been a challenge due to the quality and quantity of the extracted DNA. DNA samples extracted f ...
Ligation and Transformation
Ligation and Transformation

... – in nature, they protect bacteria from intruding DNA – cut up (restrict) the viral DNA – cut only at very specific nucleotide sequences • Restriction site: recognition sequence for a particular restriction enzyme • Restriction fragments: segments of DNA cut by restriction enzymes in a reproducible ...
Ch. 13 Genetic Engineering
Ch. 13 Genetic Engineering

... which takes advantage of naturally occurring genetic variation in plants, animals, and other organisms, to pass desired traits on to the next generation of organisms ...
APNucleic Acids
APNucleic Acids

Chapter 13 Genetics and Biotechnology
Chapter 13 Genetics and Biotechnology

... Procedures often include: cleavage by a restriction enzyme, isolation of fragments, combination with exogenous DNA, cloning or PCR and identification of ...
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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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