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Evaluation of a Novel Simple/Complex STR Multiplex for DNA
Evaluation of a Novel Simple/Complex STR Multiplex for DNA

... A novel marker system for DNA fingerprinting has been developed in Procrea's laboratories. This system presently includes seven STR markers based on Alu-tail polymorphism located on six different chromosomes. In 4 markers, the polymorphic regions consist of simple repeats. The other 3 are made of hi ...
Molecular Genetics
Molecular Genetics

... Imagine the following situation: you are about to give birth (this may be tougher for some of us than others). The brain produces the hormone oxytocin (a small protein), which causes uterine muscles to contract for childbirth. Following birth, this same hormone causes muscles in the mammary glands ...
Bio40S Review
Bio40S Review

... b) What type of point mutation would occur in TTC was changed to TTG? c) What does TTG code for? d) If TTC was changed to TTT? What does TTT code for? What type of point mutation would occur? e) If TTC was changed to ATC what does ATC code for? What type of mutation would occur? f) What type o ...
A Novel Assay for DNA-Dependent DNA Polymerase Activity
A Novel Assay for DNA-Dependent DNA Polymerase Activity

... The substrate is a heteroduplex containing an annealed region (red) for priming and a template region (blue) for elongation (A). The primer to be elongated has a ruthenium chelate (Ru) attached to the 5’ end. After elongation by the DDDP activity of the polymerase (B), the reaction is stopped with N ...
Date: Period
Date: Period

...  In eukaryotes DNA is found in nucleus on multiple linear chromosomes (a chromosome IS a strand of DNA with proteins etc. associated).  In prokaryotes DNA is not in a nucleus and is usually a single circular chromosome  Prokaryotes, viruses, and eukaryotes (yeast) can contain plasmids (small extr ...
Mr. Carpenter`s Biology RNA 10 Name
Mr. Carpenter`s Biology RNA 10 Name

... and off in response to signals from their environment; however, eukaryotes lack ________________________. o Controlling the onset of transcription  Most gene regulation in eukaryotes controls the onset of ________________________ —when RNA polymerase binds to a ________________________.  Transcrip ...
Central Dogma of Molecular Biology
Central Dogma of Molecular Biology

... CENTRAL DOGMA Nucleus DNA ...
Final exam summary 120606
Final exam summary 120606

... hypotheses are usually the suspect or that the criminal is still not found. The first hypothesis, the prosecution, is the numerator in the equation, and the defense is the denominator. The likelihood ratio then tells how much support the evidence provide, usually ranging from limited to very strong ...
DNA Extraction from Strawberries
DNA Extraction from Strawberries

... of a living thing, known as a cell, has DNA in it. In humans each of these cells have 2 copies of the DNA, but in strawberries each of these have 8 copies of the DNA (scientists call this octoploid). That means strawberries have 4 times as many copies of DNA as humans, making it 4 times easier to se ...
Test-Questions to Lab Exam 1 on the Autumn Semester of 2015
Test-Questions to Lab Exam 1 on the Autumn Semester of 2015

... 49. According to the model of double DNA helix that was suggested by Watson and Creek, it was established that one of chains would not be lost during replication and the second chain would be synthesized complementary to the first one. What way of replication is it? A. Analogous B. Identical C. Sem ...
DNA & Proteins
DNA & Proteins

Slides
Slides

... phosphate group in each residue is negatively charged at physiological pH and hence they actually repel each other. So you have some conflict here. The bases actually want to associate together but the phosphates want to be as far as possible away from each other. The net result is the twist to acco ...
DNA cloning yields multiple copies of a gene or
DNA cloning yields multiple copies of a gene or

... Once all our hummingbird DNA fragments are cloned, we have the problem of finding the DNA piece that holds our gene of interest. Explain how Nucleic Acid Hybridization will accomplish this task. [2] ...
Recombinant DNA and Genetic Engineering
Recombinant DNA and Genetic Engineering

... GE Tool Box Based on the Central Dogma and the fact that in virtually organisms the CODE is perfectly conserved almost ...
Genetic analysis is extremely powerful, but also limited in the
Genetic analysis is extremely powerful, but also limited in the

breakfast proteins
breakfast proteins

... Write out a template for the cereal chain using letters to correspond to the different colors of the cereal (ie. YOPPRRGYYOP). Tape this down somewhere in the corner of the room and section off this area with some string. Put some scrap paper and things to write with next to the template. To do and ...
Inquiry into Life Twelfth Edition
Inquiry into Life Twelfth Edition

... Enhancers and Silencers • These are position- and orientationindependent DNA elements that stimulate or depress, respectively, transcription of associated genes • Are often tissue-specific in that they rely on tissue-specific DNA-binding proteins for their activities • Some DNA elements can act eit ...
Mutations booklet MutationsAND Consequences
Mutations booklet MutationsAND Consequences

... with Tay Sachs), but in other cases this could have very minor consequences that may be harmful but not deadly (Sickle-Cell), or it could even result in a beneficial consequence (the protein is better able to do its job, or gains a new function). Single base substitution that alters a single amino a ...
Materials and Methods
Materials and Methods

Life Orientation (Grade 12 Teachers)
Life Orientation (Grade 12 Teachers)

... Building blocks of nucleic acids consisting of a sugar, a base and a phosphate The formation of an exact copy of the DNA in a cell The original strand upon which a new strand is developed The new strand that is made based on the sequence of nucleotides on the template The base that pairs off with gu ...
Summary - EUR RePub
Summary - EUR RePub

... molecule as the transcribed gene, the so-called cis-regulatory DNA elements. The other component acts in trans, i.e. it requires molecules separate from the gene containing DNA molecule, here referred to as trans-acting factors. Despite the fact that cis-regulatory DNA elements have different functi ...
chp-5 nucleic acid
chp-5 nucleic acid

DNA Technology
DNA Technology

... o _____________________________________________________ o _____________________________________________________ o _____________________________________________________ o _____________________________________________________ o ______________________________________________________ Noncoding DNA • ___ ...
doc
doc

... 1. Ask students about their answers. In discussing their answers make sure that students know that the most abundant molecule in the body is water; second is protein. The relative amounts of DNA, sugar, and fats vary depending on the person, but you could discuss which one they suspect would be most ...
Heidi Sleister
Heidi Sleister

... shows the structure of DNA (double helix, paired nitrogenous bases (G-C, A-T)). ...
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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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