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From DNA to RNA
From DNA to RNA

Removal of introns CORRECT ANSWER
Removal of introns CORRECT ANSWER

Lecture 8
Lecture 8

... gene causes reduced gene expression of that gene instead of knocking it out. The residual gene activity is due to the spicing of dSpm from pre-mRNA. However, if trans-factors TNPA is present then gene activity is knocked out i.e. pre-mRNA is not formed. TNPA binding with dSpm probably causes steric ...
COMPARISON OF THREE DNA ISOLATION AND
COMPARISON OF THREE DNA ISOLATION AND

studying genomes - Laboratory of Informatics and Chemistry
studying genomes - Laboratory of Informatics and Chemistry

... • Molecular biologists adopted these enzymes as tools for manipulating DNA molecules in pre-determined ways, using them to make copies of DNA molecules, to cut DNA molecules into shorter fragments, and to join them together again in combinations that do not exist in nature. • These manipulations for ...
15 N
15 N

... varies from species to species  all 4 bases not in equal quantity  bases present in characteristic ratio ...
VNTR, STR and RFLP
VNTR, STR and RFLP

... • VNTR – variable number tandem repeats – Location in a genome where a short nucleotide is organized as a tandem repeat – These can be found on many chromosomes and often show variations in length – Each variant acts as an inherited allele allowing used for identification – Useful in genetics, biolo ...
Online Counseling Resource YCMOU ELearning Drive…
Online Counseling Resource YCMOU ELearning Drive…

...  The code is read by copying stretches of DNA into the related nucleic acid RNA, in a process called transcription.  Most of these RNA molecules are used to synthesize proteins, but others are used directly in structures such as ribosomes and spliceosomes.  Within cells, DNA is organized into str ...
Lecture 17, Mar 3
Lecture 17, Mar 3

... between each "dG dC" pair. ...
Build Your DNA and Eat It Too
Build Your DNA and Eat It Too

... Build Your DNA and Eat It Too Objective: 1. Students will be able to describe the structure of the DNA molecule. 2. Students will be able to explain the rules of base pairing. 3. Students will understand that information is stored within the DNA molecule in the form of a sequence of chemical bases, ...
Cloning Genes
Cloning Genes

... - Insert DNA into plasmid (or phage vector) -Transform recombinant DNA molecule into bacteria -Amplify DNA by culturing transformed bacteria ...
Access Slides
Access Slides

... Structure of RNAPII and interaction of the enzyme with promoter DNA. This schematic representation of the polymerase (shown in orange) emphasizes the way in which the clamp and wall domains restrict access to the active site. Subunits Rpb4 and Rpb7 form a complex (shown in blue) that can dissociate ...
Document
Document

... C33. Methyl-directed mismatch repair is aimed at eliminating mismatches that may have occurred during DNA replication. In this case, the wrong base is in the newly made strand. The binding of MutH, which occurs on a hemimethylated sequence, provides a sensing mechanism to distinguish between the unm ...
C1. A. G→A, which is a transition. B. T→G, which is a transversion. C
C1. A. G→A, which is a transition. B. T→G, which is a transversion. C

... C33. Methyl-directed mismatch repair is aimed at eliminating mismatches that may have occurred during DNA replication. In this case, the wrong base is in the newly made strand. The binding of MutH, which occurs on a hemimethylated sequence, provides a sensing mechanism to distinguish between the unm ...
Powerpoint Presentation: Gene Expression
Powerpoint Presentation: Gene Expression

...  Fairly stable  Found in ribosomes  Made as subunits in the nucleolus  rRNA provides the platform for protein synthesis ...
Slide 1
Slide 1

... Step 1: Degenerate primers designed to DBLa conserved amino ...
nucleic acid,nursing2015 ppt
nucleic acid,nursing2015 ppt

DNA: the Genetic Material Chapter 9.1
DNA: the Genetic Material Chapter 9.1

...  Heredity – Transmission of genetic traits from parent to offspring.  Trait – Attribute in an organism controlled by genes.  I could do this all day people … mwuah ha ha ha ha!  Gene – A section of a chromosome that codes for a protein or RNA molecule.  Chromosome – Structure made of DNA & Prot ...
Isolating DNA from Fruits
Isolating DNA from Fruits

... therefore contain an abundance of DNA, making polyploid organisms practical for extracting and isolating DNA. In order to isolate DNA from fruit sources, the cell walls, cell membrane, and nuclear membranes must be disintegrated or lysed, the enzymes that naturally digest DNA must be disabled, and t ...
Cells, Chromosomes, Genes
Cells, Chromosomes, Genes

... STR (PCR) Typing • Use PCR (polymerase chain reaction) to amplify DNA • Primer sequence from locus region (locus – chromosomal location of genetic marker or repeat) ...
Genetics Notes C Molecular Genetics Vocabulary • central dogma of
Genetics Notes C Molecular Genetics Vocabulary • central dogma of

... The double helix shape of DNA, together with Chargaff’s rules, led to a better understanding of DNA. DNA, as a nucleic acid, is made from nucleotide monomers, and the DNA double helix consists of two polynucleotide chains. Each nucleotide consists of a sugar (deoxyribose), a phosphate group, and a ...
DNA topology and genome organization in higher eukaryotes
DNA topology and genome organization in higher eukaryotes

Transcription and Translation
Transcription and Translation

... added or deleted to the DNA sequence. This causes a shift in what is called the reading frame. Because DNA is read three bases at a time in a ribosome, an insertion or deletion can cause the wrong amino acids to be added to the chain. This usually results in the assembly of a nonfunctional protein. ...
Genetics I Exam 5 Review Sheet - Poultry Science
Genetics I Exam 5 Review Sheet - Poultry Science

... 44. Can DNA replication and transcription proceed if a pyrimidine dimer is formed? 45. A genetic disorder of DNA repair in which the body's normal ability to remove damage caused by ultraviolet light is deficient. This leads to multiple basaliomas and other skin malignancies at a young age. In sever ...
Document
Document

... • One of the greatest achievements of modern science has been the sequencing of the human genome, which was largely completed by 2003 • DNA sequencing accomplishments – Have all depended on advances in DNA technology, starting with the invention of methods for making recombinant DNA – DNA sequencing ...
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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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