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Biochemical Analysis of the Human Mismatch Repair Proteins
Biochemical Analysis of the Human Mismatch Repair Proteins

... is the nucleoside diphosphate kinase (NDPK) activity, which catalyzes transfer of γ-phosphate between nucleoside triphosphates and diphosphates. The second one is the 3'-5' exonuclease activity, which is involved in repair of UV-induced DNA damage in yeasts. UV radiation is one of the most widesprea ...
Genetic Engineering Techniques
Genetic Engineering Techniques

File - Personal FSU Notes
File - Personal FSU Notes

... Unlike viruses, DNA is active even though it is highly compacted within the cell ...
Recombinant DNA technology engineering) involves combining genes from genes.
Recombinant DNA technology engineering) involves combining genes from genes.

... the late 1960s. •In nature, bacteria use restriction enzymes to cut up intruder DNA from phages and from other organisms into nonfunctional pieces. The bacteria first chemically modify their own DNA so that it will not be cut. •Several hundred different restriction enzymes have been discovered that ...
Exam 2
Exam 2

... Know the difference between DNA and RNA concerning the number of strands and the nucleotides associated with each nucleic acid. What is the purpose of transcription and translation? Where does each process occur (nucleus or cytoplasm)? What is RNA polymerase and what is its function? What is a codon ...
File
File

... 4) In trying to determine whether DNA or protein is the genetic material, Hershey and Chase made use of which of the following facts? A) DNA contains sulfur, whereas protein does not. B) DNA contains phosphorus, whereas protein does not. C) DNA contains nitrogen, whereas protein does not. D) DNA co ...
Chapter 10
Chapter 10

DNA polymerase - yusronsugiarto
DNA polymerase - yusronsugiarto

A rough guide to molecular biology.
A rough guide to molecular biology.

... thymine and guanine to cytosine) so that the base sequence of one strand defines that of the other. They are anti-parallel because the orientation of their 59 to 39 directions are opposite. The strands are held together by hydrogen bonds and hydrophobic interactions. This relatively weak bonding mea ...
Solving the structure of DNA
Solving the structure of DNA

... Because of this, in a complex organism, evolution will select against low fidelity DNA replication. The structure of DNA suggested a way that it could be replicated with high fidelity. Because the strands are complementary, one strand could specify the base on the opposite strand. This is actually w ...
DNA upgrade supplement WITH PICS
DNA upgrade supplement WITH PICS

... sugar molecules. This helps to describe molecules and discuss where specific bonds form. For instance the DNA sugar, deoxyribose, is easily distinguished from the RNA sugar, ribose, because deoxyribose lacks an oxygen atom at the number two position in the ring. The phosphodiester bonds Levene descr ...
video slide - BiologyAlive.com
video slide - BiologyAlive.com

... sugar-phosphate sequences called restriction the backbones at each arrow. sites – fragments with “sticky ends” ...
Reproduction in Bacteria
Reproduction in Bacteria

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DNA Evidence
DNA Evidence

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Nucleic Acids and the Genetic Code

LIMITED DNA SYNTHESIS IN THE ABSENCE OF PROTEIN
LIMITED DNA SYNTHESIS IN THE ABSENCE OF PROTEIN

... bursts of DNA synthesis. These bursts of DNA synthesis can be described as "rounds" of replication, because this term is consistent with the temporal order of the replication process. A round of replication is defined as the quantity of DNA synthesized after adding inhibitor during the S phase. From ...
Chapter 12
Chapter 12

... 3. RNA splicing Gene expression can be controlled by altering the rate of splicing in eukaryotes. Alternative splicing can produce multiple mRNAs from one gene. ...
Gene expression flash cards
Gene expression flash cards

... codes for an amino acid mRNA ...
Topic 10: Inheritance/Genetics, or Why do we resemble our
Topic 10: Inheritance/Genetics, or Why do we resemble our

... Complementary base pairing A = T (also A = U) Lines represent “hydrogen bonds” between bases (+/attraction) G=C ...
Document
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... • GFP is a visual marker • Study of biological processes (example: synthesis of proteins) • Localization and regulation of gene expression • Cell movement • Cell fate during development • Formation of different organs • Screenable marker to identify transgenic organisms ...
Chapter 11 – What is DNA and how does it work?
Chapter 11 – What is DNA and how does it work?

2017 DNA Lab Programmes Booklet
2017 DNA Lab Programmes Booklet

... Application of bioinformatics to perform nucleotide homology searches ...
Chapter 24
Chapter 24

... An enhancer activates the nearest promoter to it, and can be any distance either upstream or downstream of the promoter. A UAS (upstream activator sequence) in yeast behaves like an enhancer but works only upstream of the promoter. Similar sequence elements are found in enhancers and promoters. Enha ...
Molecular Biology 240386
Molecular Biology 240386

... -activators and repressors that bind to specific sites in DNA and regulate expression by two mechanisms 1-regulatory proteins act in concert with other proteins to modulate chromatin structure, thereby influencing the ability of general transcription factors to bind to promoters. -DNA in eukaryotic ...
Chapter 12
Chapter 12

... 1. Genes of most eukaryotes have interrupted coding sequences a) Coding sequences are exons (expressed sequences); noncoding sequences are introns (intervening sequences) b) A typical eukaryotic gene has multiple exons and introns, with variable numbers of each 2. This terminology refers to sequence ...
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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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