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Stress Responses and Checkpoints for DNA Damage
Stress Responses and Checkpoints for DNA Damage

... Ataxia-telangiectasia is a rare, childhood neurological disorder that causes degeneration in the part of the brain that controls motor movements and speech. Its most unusual symptom is an acute sensitivity to ionizing radiation, such as X-rays or gamma-rays. The first signs of the disease, which inc ...
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... Synthesize a primer (17-24mer) to a predetermined segment of a cloning vector near the cloned DNA, the primer is annealed to the cloned DNA. The reactions are carried out in 4 separate tubes each with different ddNTP. In the tube w/ ddATP whose concen. is carefully controlled so ddNTP is incorporat ...
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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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