Restriction Enzymes
... of the phosphodiester bonds within both strands of DNA. • They require Mg+2 for activity and generate a 5 prime (5') phosphate and a 3 prime (3') hydroxyl group at the point of cleavage. ...
... of the phosphodiester bonds within both strands of DNA. • They require Mg+2 for activity and generate a 5 prime (5') phosphate and a 3 prime (3') hydroxyl group at the point of cleavage. ...
Chapter 14 - TeacherWeb
... one DNA strand is used as backbone of the other strand a template. of nucleotides Transcription is initiated at a transcribed DNA promoter, a DNA base winds up again sequence that signals the start of a gene. Newly forming RNA transcript ...
... one DNA strand is used as backbone of the other strand a template. of nucleotides Transcription is initiated at a transcribed DNA promoter, a DNA base winds up again sequence that signals the start of a gene. Newly forming RNA transcript ...
The Genetic Code The nucleotide bases of the DNA strand
... of complementary nucleotide bases. This time, however, small molecules with t h r e e nucleotide bases have to complement the sequence on the mRNA. This RNA is called the transfer-RNA (tRNA), and it can only accomplish a complementation, when all t h r e e bases find tree adjacent, matching bases on ...
... of complementary nucleotide bases. This time, however, small molecules with t h r e e nucleotide bases have to complement the sequence on the mRNA. This RNA is called the transfer-RNA (tRNA), and it can only accomplish a complementation, when all t h r e e bases find tree adjacent, matching bases on ...
Social media policy
... The number of bases that are read at one time (that is the number of letters that will appear in each read). This differs between technologies, so optimum fragment length varies. Recessive allele A gene variant in one copy of a pair of genes that will not affect the individual. Reference genome An e ...
... The number of bases that are read at one time (that is the number of letters that will appear in each read). This differs between technologies, so optimum fragment length varies. Recessive allele A gene variant in one copy of a pair of genes that will not affect the individual. Reference genome An e ...
Ligation and Transformation
... which bacterial cells contain the antibiotic resistant plasmid insert & which do not • Example: bacterium containing a plasmid with resistance to a particular antibiotic (ampicillin) will grow on medium that ...
... which bacterial cells contain the antibiotic resistant plasmid insert & which do not • Example: bacterium containing a plasmid with resistance to a particular antibiotic (ampicillin) will grow on medium that ...
![mutations[1]](http://s1.studyres.com/store/data/008317487_1-c5116f8f771ed5816060cc76bc28009f-300x300.png)
mutations[1]
... UV light can induce adjacent thymine bases in a DNA strand to pair with each other, as a bulky dimer. DNA has so-called hotspots, where mutations occur up to 100 times more frequently than the normal mutation rate. A hotspot can be at an unusual base, e.g., 5-methylcytosine. Mutation rates also ...
... UV light can induce adjacent thymine bases in a DNA strand to pair with each other, as a bulky dimer. DNA has so-called hotspots, where mutations occur up to 100 times more frequently than the normal mutation rate. A hotspot can be at an unusual base, e.g., 5-methylcytosine. Mutation rates also ...
2. You perform a Southern blot in which your probe should hybridize
... 1. Inadequate prehybridization (regions of membrane were left unblocked) 2. You touched the membrane with ungloved hands. NOTE: This would be because probe stuck to oils or other debris from your hands. It would NOT be because DNA from cells on your fingers hybridized to the probe. {Why? Even if the ...
... 1. Inadequate prehybridization (regions of membrane were left unblocked) 2. You touched the membrane with ungloved hands. NOTE: This would be because probe stuck to oils or other debris from your hands. It would NOT be because DNA from cells on your fingers hybridized to the probe. {Why? Even if the ...
Lecture#22 - Cloning DNA and the construction of clone libraries
... 1) Origin of replication -similar in function to oriC in E. coli chromosome 2) Selectable marker gene - usually an antibiotic resistance gene - e.g. ampR, tetR, kanR 3) Multiple cloning site (MCS) – many restriction enzyme sites in a short sequence. ...
... 1) Origin of replication -similar in function to oriC in E. coli chromosome 2) Selectable marker gene - usually an antibiotic resistance gene - e.g. ampR, tetR, kanR 3) Multiple cloning site (MCS) – many restriction enzyme sites in a short sequence. ...
The Body in Motion
... bacteria and the bacteria allowed to grow • This will produce many genetically identical copies of the piece of DNA. This is called cloning • A clone is a genetically identical individual or cell ...
... bacteria and the bacteria allowed to grow • This will produce many genetically identical copies of the piece of DNA. This is called cloning • A clone is a genetically identical individual or cell ...
Biotechnology and Recombinant DNA I. Tools of Biotechnology
... portion of the Ti plasmid (T-DNA) is transferred into the plant cell. • This system has been well characterized and is now used to introduce foreign DNA into plants as well as some animal cells. iv. Screening or Detection of Recombinant Molecules • May be creating a scenario not much different than ...
... portion of the Ti plasmid (T-DNA) is transferred into the plant cell. • This system has been well characterized and is now used to introduce foreign DNA into plants as well as some animal cells. iv. Screening or Detection of Recombinant Molecules • May be creating a scenario not much different than ...
BIOLOGY (Theory)
... Ans: Stanley Cohen and Herbert Boyer conducted one of the first genetic engineering experiments. They invented the technique of DNA cloning. Cohen developed a method of removing plasmids from the cell and then reinserting them in other cells. Combining this process with that of DNA splicing enabled ...
... Ans: Stanley Cohen and Herbert Boyer conducted one of the first genetic engineering experiments. They invented the technique of DNA cloning. Cohen developed a method of removing plasmids from the cell and then reinserting them in other cells. Combining this process with that of DNA splicing enabled ...
Unit 5 DNA, RNA and Protein Synthesis
... helix as a template. The double-stranded molecule of DNA separates along the hydrogen bonds. An enzyme called RNA polymerase adds in corresponding base pairs. However, instead of using Thymine to match up with Adenine, Uracil is used. For RNA, the base paring rules are A-U and G-C. At the end of thi ...
... helix as a template. The double-stranded molecule of DNA separates along the hydrogen bonds. An enzyme called RNA polymerase adds in corresponding base pairs. However, instead of using Thymine to match up with Adenine, Uracil is used. For RNA, the base paring rules are A-U and G-C. At the end of thi ...
DNA: The Molecule of Life - Calgary Christian School
... sequence called the replication origin 2) During replication, weak hydrogen bonds that hold complementary nitrogen bases together are broken (This causes the two edges to “unzip”) with a special group of enzymes called helicases (gyrase breaks the hydrogen bonds) 3) This creates two y-shaped areas ( ...
... sequence called the replication origin 2) During replication, weak hydrogen bonds that hold complementary nitrogen bases together are broken (This causes the two edges to “unzip”) with a special group of enzymes called helicases (gyrase breaks the hydrogen bonds) 3) This creates two y-shaped areas ( ...
Transcription
... growing RNA chain, sigma factor is released and reused for other initiations. Core enzyme completes the transcript (Figure 5.4). 2. Core enzyme untwists DNA helix locally, allowing a small region to denature. Newly synthesized RNA forms an RNA-DNA hybrid, but most of the transcript is displaced as t ...
... growing RNA chain, sigma factor is released and reused for other initiations. Core enzyme completes the transcript (Figure 5.4). 2. Core enzyme untwists DNA helix locally, allowing a small region to denature. Newly synthesized RNA forms an RNA-DNA hybrid, but most of the transcript is displaced as t ...
Answers to Conceptual Questions C1. Answer: First
... of DNA from a source of chromosomal DNA. Because it is a diverse collection of many different DNA pieces, the name library seems appropriate. E15. Answer: It would be necessary to use cDNA so that the gene would not carry any introns. Bacterial cells do not contain spliceosomes (which are described ...
... of DNA from a source of chromosomal DNA. Because it is a diverse collection of many different DNA pieces, the name library seems appropriate. E15. Answer: It would be necessary to use cDNA so that the gene would not carry any introns. Bacterial cells do not contain spliceosomes (which are described ...
12) Inheritance, genes and chromosomes • 13) DNA
... • Synthesis of the lagging strand occurs in small, discontinuous stretches—Okazaki fragments. • Each Okazaki fragment requires its own primer, synthesized by the primase. • DNA polymerase III adds nucleotides to the 3′ end, until reaching the primer of the previous fragment. • DNA polymerase I t ...
... • Synthesis of the lagging strand occurs in small, discontinuous stretches—Okazaki fragments. • Each Okazaki fragment requires its own primer, synthesized by the primase. • DNA polymerase III adds nucleotides to the 3′ end, until reaching the primer of the previous fragment. • DNA polymerase I t ...
DNA - Biology
... After the PCR reaction, the resulting product is analyzed in another technique called gel electrophoresis. In gel electrophoresis, the DNA product can be sorted by size and the lengths of the VNTR regions can then be determined. The apparatus for a gel electrophoresis consists of a container filled ...
... After the PCR reaction, the resulting product is analyzed in another technique called gel electrophoresis. In gel electrophoresis, the DNA product can be sorted by size and the lengths of the VNTR regions can then be determined. The apparatus for a gel electrophoresis consists of a container filled ...
What is Biology? The word biology is 1………………………. from the
... sequences of DNA known as 7……………………………. regions. These regions may be thousands of base pairs distant from the start of the gene. Contact between the activator proteins and the initiationcomplex releases the copying mechanism. The RNA polymerase 8……………………………. a small portion of the DNA helix exposin ...
... sequences of DNA known as 7……………………………. regions. These regions may be thousands of base pairs distant from the start of the gene. Contact between the activator proteins and the initiationcomplex releases the copying mechanism. The RNA polymerase 8……………………………. a small portion of the DNA helix exposin ...
Sample IHC Normal Expression Report Reason For Referral
... possibility that this individual's tumor is due to an inherited defect in another gene not involved in DNA mismatch repair. A significant fraction of clinically defined HNPCC cases (30% or more) do not have defective DNA mismatch repair as the underlying genetic basis of their disease. Additionally, ...
... possibility that this individual's tumor is due to an inherited defect in another gene not involved in DNA mismatch repair. A significant fraction of clinically defined HNPCC cases (30% or more) do not have defective DNA mismatch repair as the underlying genetic basis of their disease. Additionally, ...
Replisome
The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.