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Supplemental Figure 3. Spurious amplification of SCR1 from genomic DNA of representative A. thaliana acccessions. Equivalent amounts of genomic DNA isolated from different accessions were subjected to PCR using the same pair of SCR1 primers (the PseSCR3 and PseSCR5 primers described by Shimizu et al. 2004). Note that DNA from the C24 and Mt-0 accessions, which lack SCR1 (Table 1 and Figure 3), produce faint amplification products (asterisk), presumably due to annealing of the primers to non-SCR1 sequences. We were unable to sequence these spurious PCR products directly, probably because they consist of a heterogeneous mix of sequences. This conclusion was confirmed by sequencing individual clones obtained by TA cloning of the spurious C24 PCR products in the pGEMT plasmid, which returned sequences corresponding to various genomic regions.