Biotechnology - clevengerscience
... Sequencing DNA • A PCR is run four times; each time with a different dideoxynucleotide, or “ddNTP,” which lack the 3'hydroxyl group. • Whenever a ddNTP is incorporated into a growing DNA chain, it stops chain ...
... Sequencing DNA • A PCR is run four times; each time with a different dideoxynucleotide, or “ddNTP,” which lack the 3'hydroxyl group. • Whenever a ddNTP is incorporated into a growing DNA chain, it stops chain ...
Molecular Basis of Heredity
... 1. The search for genetic material led to DNA • Once T.H. Morgan’s group showed that genes are located on chromosomes, the two constituents of chromosomes - proteins and DNA - were the candidates for the genetic material. • Until the 1940s, the great heterogeneity and specificity of function of pro ...
... 1. The search for genetic material led to DNA • Once T.H. Morgan’s group showed that genes are located on chromosomes, the two constituents of chromosomes - proteins and DNA - were the candidates for the genetic material. • Until the 1940s, the great heterogeneity and specificity of function of pro ...
Genomics
... “inheritance of acquired characteristics”, in which traits acquired by parents could be passed on to successive generations. Although usually attributed to Jean Baptiste Lamarck, it was a commonly accepted method of inheritance in the late 19th and early 20th centuries. The rise of the “modern synth ...
... “inheritance of acquired characteristics”, in which traits acquired by parents could be passed on to successive generations. Although usually attributed to Jean Baptiste Lamarck, it was a commonly accepted method of inheritance in the late 19th and early 20th centuries. The rise of the “modern synth ...
Biol120 Mock Final Examination
... 41. C. Botulinum is a rod-shaped bacterium that produces a toxin that causes botulism in humans. Most botulism cases occur when food that was improperly canned is eaten. These bacteria can only grow in the oxygen-free environment of a sealed can, and the moist food provides the bacteria with a ready ...
... 41. C. Botulinum is a rod-shaped bacterium that produces a toxin that causes botulism in humans. Most botulism cases occur when food that was improperly canned is eaten. These bacteria can only grow in the oxygen-free environment of a sealed can, and the moist food provides the bacteria with a ready ...
DNA and Proteins - Furman University
... build a complementary strand of RNA. This process of reading DNA and making RNA is called 'transcription'. This is a great word for the process, as the message written in the language of nucleic acids is copied in essentially the same language - the language of nucleic acids. This RNA may be a recip ...
... build a complementary strand of RNA. This process of reading DNA and making RNA is called 'transcription'. This is a great word for the process, as the message written in the language of nucleic acids is copied in essentially the same language - the language of nucleic acids. This RNA may be a recip ...
Chapter 9 - HCC Learning Web
... synthesis of new strands and the reassembly of double-stranded molecules. d. the use of the original double-stranded molecule as a template, without unwinding. e. none of the above. The feature of the Watson-Crick model of DNA structure that explains its ability to function in replication is a. that ...
... synthesis of new strands and the reassembly of double-stranded molecules. d. the use of the original double-stranded molecule as a template, without unwinding. e. none of the above. The feature of the Watson-Crick model of DNA structure that explains its ability to function in replication is a. that ...
(3) Ch 6 Review Game
... This is the process that identifies the unique patterns in a person’s DNA. It can be helpful in many areas, including solving crimes and ...
... This is the process that identifies the unique patterns in a person’s DNA. It can be helpful in many areas, including solving crimes and ...
Chapter 12 Rev
... synthesis of new strands and the reassembly of double-stranded molecules. d. the use of the original double-stranded molecule as a template, without unwinding. e. none of the above. The feature of the Watson-Crick model of DNA structure that explains its ability to function in replication is a. that ...
... synthesis of new strands and the reassembly of double-stranded molecules. d. the use of the original double-stranded molecule as a template, without unwinding. e. none of the above. The feature of the Watson-Crick model of DNA structure that explains its ability to function in replication is a. that ...
CHAPTER 18
... • Nucleic acid hybridization is based on the ability of two complementary DNA strands to form a double-stranded hybrid. • The Southern blot technique is based upon DNA hybridization. • The Northern blot technique is based upon RNA-DNA hybridization. • Hybridization can be used to determine the degre ...
... • Nucleic acid hybridization is based on the ability of two complementary DNA strands to form a double-stranded hybrid. • The Southern blot technique is based upon DNA hybridization. • The Northern blot technique is based upon RNA-DNA hybridization. • Hybridization can be used to determine the degre ...
PDF file - the Houpt Lab
... Therefore, phage must have genetic blue prints and uses bacteria to assemble new viruses from genetic plan. Phage viruses are composed of DNA and protein. Is the genetic material in the protein, or in the DNA? ...
... Therefore, phage must have genetic blue prints and uses bacteria to assemble new viruses from genetic plan. Phage viruses are composed of DNA and protein. Is the genetic material in the protein, or in the DNA? ...
MS Word file
... A variety of different consensus sequences may be found in the regulatory promoters. Main difference between prokaryotes and eukaryotes is in assembly of ...
... A variety of different consensus sequences may be found in the regulatory promoters. Main difference between prokaryotes and eukaryotes is in assembly of ...
information transfer in life - The Origin and Evolution of Life
... they do not accurately model how information in life changes because they do not take into account the fact that amino acid changes are caused by changes in DNA. This chapter will explore how DNA stores information, and how this information is used to build proteins. It will also explore how mutatio ...
... they do not accurately model how information in life changes because they do not take into account the fact that amino acid changes are caused by changes in DNA. This chapter will explore how DNA stores information, and how this information is used to build proteins. It will also explore how mutatio ...
Spatial ordering and abnormal optical activity of DNA liquid
... LCD particles can be achieved under hexagonal packing. However, this is not a true crystal, because there are disordered water molecules between the ds DNA molecules. The ds DNA molecules possess some disorder around their positions; they can slide and bent with respect of each other, as well as the ...
... LCD particles can be achieved under hexagonal packing. However, this is not a true crystal, because there are disordered water molecules between the ds DNA molecules. The ds DNA molecules possess some disorder around their positions; they can slide and bent with respect of each other, as well as the ...
Chapter 21
... 1. Two strands unwind by breaking the H bonds 2. Complementary nucleotides are added to each strand by DNA polymerase 3. Each new double-stranded helix is made of one new strand and one old strand (semiconservative replication) **The sequence of bases makes each individual unique ...
... 1. Two strands unwind by breaking the H bonds 2. Complementary nucleotides are added to each strand by DNA polymerase 3. Each new double-stranded helix is made of one new strand and one old strand (semiconservative replication) **The sequence of bases makes each individual unique ...
BDOL Interactive Chalkboard - Broken Arrow Public Schools
... • DNA, the genetic material of organisms, is composed of four kinds of nucleotides. A DNA molecule consists of two strands of nucleotides with sugars and phosphates on the outside and bases paired by hydrogen bonding on the inside. The paired strands form a twisted-zipper shape called a double ...
... • DNA, the genetic material of organisms, is composed of four kinds of nucleotides. A DNA molecule consists of two strands of nucleotides with sugars and phosphates on the outside and bases paired by hydrogen bonding on the inside. The paired strands form a twisted-zipper shape called a double ...
bio Chapter 11 TEST (2010)
... ____ 25. Suppose a restriction enzyme recognizes the six-base sequence AAGCTT TTCGAA in a double strand of DNA. Between which two nucleotides on each strand would the enzyme have to cut to produce a fragment with sticky ends that are four bases long? a. GC b. CT c. AA d. AG ____ 26. If two DNA sampl ...
... ____ 25. Suppose a restriction enzyme recognizes the six-base sequence AAGCTT TTCGAA in a double strand of DNA. Between which two nucleotides on each strand would the enzyme have to cut to produce a fragment with sticky ends that are four bases long? a. GC b. CT c. AA d. AG ____ 26. If two DNA sampl ...
Genetics of bacteria
... complementary. Because of complementarity, doublestranded DNA contains equimolar amounts of purines (A + G) and pyrimidines (T + C), with A equal to T and G equal to C, but the mole fraction of G + C in DNA varies widely among different bacteria. Information in nucleic acids is encoded by the ordere ...
... complementary. Because of complementarity, doublestranded DNA contains equimolar amounts of purines (A + G) and pyrimidines (T + C), with A equal to T and G equal to C, but the mole fraction of G + C in DNA varies widely among different bacteria. Information in nucleic acids is encoded by the ordere ...
1 Basic Genomics 1. How do you sequence DNA? Two methods
... 3. Laser detects fluorescence automatically as each fragment exits capillary 4. Computer software “calls bases” and processes sequence files (if sequences were processed by human at 15 min. per sample, it would take 7 people a full-time week to process 1 day’s output from an automated sequencer) The ...
... 3. Laser detects fluorescence automatically as each fragment exits capillary 4. Computer software “calls bases” and processes sequence files (if sequences were processed by human at 15 min. per sample, it would take 7 people a full-time week to process 1 day’s output from an automated sequencer) The ...
COAS_B1_Ch08 Nucleic acids
... They twist around each other to form a double helix. DNA molecules in a cell nucleus are replicated before cell division takes place. First, the two • The strands of the molecule are untwisted and unzipped. Free DNA nucleotides pair up with the exposed bases on both strands. They are then linked tog ...
... They twist around each other to form a double helix. DNA molecules in a cell nucleus are replicated before cell division takes place. First, the two • The strands of the molecule are untwisted and unzipped. Free DNA nucleotides pair up with the exposed bases on both strands. They are then linked tog ...
Restriction Enzymes
... of the phosphodiester bonds within both strands of DNA. • They require Mg+2 for activity and generate a 5 prime (5') phosphate and a 3 prime (3') hydroxyl group at the point of cleavage. ...
... of the phosphodiester bonds within both strands of DNA. • They require Mg+2 for activity and generate a 5 prime (5') phosphate and a 3 prime (3') hydroxyl group at the point of cleavage. ...
Replisome
The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.