GENETIC TECHNOLOGY
... Copy DNA without vectors and host cells Goal to make many copies of DNA in a defined region Uses high concentration of two primers that are complementary to sequences at the ends of the DNA region to be amplified, deoxynucleoside triphosphates (dNTPs), and a heat-stable form of DNA polymerase called ...
... Copy DNA without vectors and host cells Goal to make many copies of DNA in a defined region Uses high concentration of two primers that are complementary to sequences at the ends of the DNA region to be amplified, deoxynucleoside triphosphates (dNTPs), and a heat-stable form of DNA polymerase called ...
Biology EOC Review Pack
... Goal 3: Learner will develop an understanding of the continuity of life and the changes of organisms over time. 3.01 Analyze the molecular basis of heredity. 36) Describe the structure of DNA. Who discovered this structure? 37) Draw and label a DNA strand 2 nucleotides long. 38) Name the nitrogenous ...
... Goal 3: Learner will develop an understanding of the continuity of life and the changes of organisms over time. 3.01 Analyze the molecular basis of heredity. 36) Describe the structure of DNA. Who discovered this structure? 37) Draw and label a DNA strand 2 nucleotides long. 38) Name the nitrogenous ...
RNA and Protein Synthesis - Port Washington School District
... acids to the ribosomes where they are eventually assembled into protein chains – Each amino acid is coded for by a different triplet codon on mRNA – tRNA has an anticodon that will pair up with codon on mRNA ...
... acids to the ribosomes where they are eventually assembled into protein chains – Each amino acid is coded for by a different triplet codon on mRNA – tRNA has an anticodon that will pair up with codon on mRNA ...
Section 12-1
... TATA box – a short region of DNA about 30 base pairs long; seems to help position RNA polymerase by marking a point just before the point at which transcription begins ...
... TATA box – a short region of DNA about 30 base pairs long; seems to help position RNA polymerase by marking a point just before the point at which transcription begins ...
Isolating Hereditary Material: Frederick Griffith
... To determine the roles that the T2 bacteriophage's DNA and Figure 4 protein play in infection, Hershey and Chase decided to use radioisotopes to trace the fate of the phage's protein and DNA by taking advantage of their chemical differences. Proteins contain sulfur, but DNA does not. Conversely, DNA ...
... To determine the roles that the T2 bacteriophage's DNA and Figure 4 protein play in infection, Hershey and Chase decided to use radioisotopes to trace the fate of the phage's protein and DNA by taking advantage of their chemical differences. Proteins contain sulfur, but DNA does not. Conversely, DNA ...
Nucleotides and Nucleic Acids
... strand. Calculate the total (net) bend produced in a DNA if the center base pairs (the third of five) of two successive (dA)5 tracts are located (a) 10 base pairs apart; (b) 15 base pairs apart. Assume 10 base pairs per turn in the DNA double helix. Answer When bending elements are repeated in phase ...
... strand. Calculate the total (net) bend produced in a DNA if the center base pairs (the third of five) of two successive (dA)5 tracts are located (a) 10 base pairs apart; (b) 15 base pairs apart. Assume 10 base pairs per turn in the DNA double helix. Answer When bending elements are repeated in phase ...
8000 - International Commission on Missing Persons
... findings of ICMP’s forensic work related to Srebrenica event. To date, a total of 20 individuals have been tried at the ICTY for crimes related to Srebrenica over the course of 12 cases. Of these, 14 individuals were convicted and one was acquitted. Three cases are ongoing: one is awaiting Trial Cha ...
... findings of ICMP’s forensic work related to Srebrenica event. To date, a total of 20 individuals have been tried at the ICTY for crimes related to Srebrenica over the course of 12 cases. Of these, 14 individuals were convicted and one was acquitted. Three cases are ongoing: one is awaiting Trial Cha ...
Document
... buffer (salt, pH) for enzyme to work. Mimics cellular conditions of bacteria they come from. ...
... buffer (salt, pH) for enzyme to work. Mimics cellular conditions of bacteria they come from. ...
Document
... Termination RNA polymerase recognizes signals for chain termination. (1) Intrinsic: Termination site on template DNA consists of GC-rich sequences followed by A’s. Intramolecular hydrogen bonding causes formation of hairpin loop. ...
... Termination RNA polymerase recognizes signals for chain termination. (1) Intrinsic: Termination site on template DNA consists of GC-rich sequences followed by A’s. Intramolecular hydrogen bonding causes formation of hairpin loop. ...
Ways to detect unique sequences within mammalian DNA
... EX: humans have 3 billion base pairs with 1 million restriction fragments formed from a single restriction enzyme digest - TOO difficult to isolate a single band on a gel from this large number of fragments To characterize a specific gene use blot hybridization - see Figure 1 - WE DID THIS!! ...
... EX: humans have 3 billion base pairs with 1 million restriction fragments formed from a single restriction enzyme digest - TOO difficult to isolate a single band on a gel from this large number of fragments To characterize a specific gene use blot hybridization - see Figure 1 - WE DID THIS!! ...
Chapter 6B
... different regions of chromosomes forming distinctive bands. For Giemsa reagent, banding is affected by G + C content. Banding patterns are very important in chromosome ID and in looking for chromosomal abnormalities and mapping the locations of genes. The most detailed staining is achieved via multi ...
... different regions of chromosomes forming distinctive bands. For Giemsa reagent, banding is affected by G + C content. Banding patterns are very important in chromosome ID and in looking for chromosomal abnormalities and mapping the locations of genes. The most detailed staining is achieved via multi ...
12 RNA Activity
... Place the fluorescent tag on the probe backbone that you think will work Attach your probe backbone on top of the bacteria bases. What problems did you have to solve to attach it? Did it ...
... Place the fluorescent tag on the probe backbone that you think will work Attach your probe backbone on top of the bacteria bases. What problems did you have to solve to attach it? Did it ...
AP BIO Unit 6 - DNA History
... ‘Rungs” of the ladder are nitrogenous bases connected by hydrogen bonds AP Biology ...
... ‘Rungs” of the ladder are nitrogenous bases connected by hydrogen bonds AP Biology ...
CST Review
... 45. The gene for color vision (C) is dominant to the gene for color blindness (c) and is located on the X chromosome. If a color blind man and a woman with homozygous normal color vision have children, what are the chances that they will have a colorblind child? 46. Why do some lethal (deadly) allel ...
... 45. The gene for color vision (C) is dominant to the gene for color blindness (c) and is located on the X chromosome. If a color blind man and a woman with homozygous normal color vision have children, what are the chances that they will have a colorblind child? 46. Why do some lethal (deadly) allel ...
Chapter 17: From Gene to Protein
... phenomenon explains the fact that there are only about 45 different tRNA molecules that pair with the 61 possible codons (three codons are always stop codons). The third nucleotide of many tRNAs can pair with more than one base. Because of the redundancy of the genetic code, these wobble tRNAs still ...
... phenomenon explains the fact that there are only about 45 different tRNA molecules that pair with the 61 possible codons (three codons are always stop codons). The third nucleotide of many tRNAs can pair with more than one base. Because of the redundancy of the genetic code, these wobble tRNAs still ...
Nucleotides and Nucleic Acids DNA and RNA
... Nucleic acids are viewed is the same way Primary structure: order of bases in polynucleotide Secondary structure: 3D conformation of the backbone Tertiary structure: supercoiling of the molecule ...
... Nucleic acids are viewed is the same way Primary structure: order of bases in polynucleotide Secondary structure: 3D conformation of the backbone Tertiary structure: supercoiling of the molecule ...
Chapter 13 Vocabulary Name
... 14. promoter: control sequence on an operon where RNA polymerase attaches to the DNA (Concept 13.5) 15. operator: control sequence on an operon that acts as a switch, determining whether or not RNA polymerase can attach to the promoter (Concept 13.5) 16. repressor: protein that binds to the operator ...
... 14. promoter: control sequence on an operon where RNA polymerase attaches to the DNA (Concept 13.5) 15. operator: control sequence on an operon that acts as a switch, determining whether or not RNA polymerase can attach to the promoter (Concept 13.5) 16. repressor: protein that binds to the operator ...
Go Bananas
... pipette may help. Don’t mix the alcohol and banana solution. Add about the same amount of alcohol as banana fluid. Ice-cold 100% ethanol works best: perhaps because the temperature gradient helps the DNA to precipitate. 12 Replace the lid and GENTLY swirl the tube a few times. 13 Look at where the t ...
... pipette may help. Don’t mix the alcohol and banana solution. Add about the same amount of alcohol as banana fluid. Ice-cold 100% ethanol works best: perhaps because the temperature gradient helps the DNA to precipitate. 12 Replace the lid and GENTLY swirl the tube a few times. 13 Look at where the t ...
VGEC: Teacher Notes Go Bananas!
... pipette may help. Don’t mix the alcohol and banana solution. Add about the same amount of alcohol as banana fluid. Ice-cold 100% ethanol works best: perhaps because the temperature gradient helps the DNA to precipitate. 12 Replace the lid and GENTLY swirl the tube a few times. 13 Look at where the t ...
... pipette may help. Don’t mix the alcohol and banana solution. Add about the same amount of alcohol as banana fluid. Ice-cold 100% ethanol works best: perhaps because the temperature gradient helps the DNA to precipitate. 12 Replace the lid and GENTLY swirl the tube a few times. 13 Look at where the t ...
1. (a) When a cell divides, the genetic material can divide by mitosis
... The inheritance of the ability to produce hydrogen cyanide is controlled by two genes which are located on different chromosomes. The dominant allele of one gene, G, controls the production of enzyme G which converts a precursor to linamarin. The dominant allele of the other gene, E, controls the pr ...
... The inheritance of the ability to produce hydrogen cyanide is controlled by two genes which are located on different chromosomes. The dominant allele of one gene, G, controls the production of enzyme G which converts a precursor to linamarin. The dominant allele of the other gene, E, controls the pr ...
8.4 Transcription - School District of La Crosse
... 8.4 Transcription • Transcription makes three types of RNA. – Messenger RNA (mRNA) carries the message that will be translated to form a protein. – Ribosomal RNA (rRNA) forms part of ribosomes where proteins are made. – Transfer RNA (tRNA) brings amino acids from the cytoplasm to a ribosome. ...
... 8.4 Transcription • Transcription makes three types of RNA. – Messenger RNA (mRNA) carries the message that will be translated to form a protein. – Ribosomal RNA (rRNA) forms part of ribosomes where proteins are made. – Transfer RNA (tRNA) brings amino acids from the cytoplasm to a ribosome. ...
Replisome
The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.