Principles of Virology
... Replication is divided in two distinct steps linked to the differentiation state of the host epithelial cell: a) Basal squamous epithelial cells: Plasmid/Episome replication. It is in synchrony with the host cell chromosome in order to ensures an average of one viral genome per basal cell. b) Differ ...
... Replication is divided in two distinct steps linked to the differentiation state of the host epithelial cell: a) Basal squamous epithelial cells: Plasmid/Episome replication. It is in synchrony with the host cell chromosome in order to ensures an average of one viral genome per basal cell. b) Differ ...
DNA
... amount of negative and positive charges. This is the isoelectric point of the protein, at which it has no net charge. • For isoelectric focusing, the proteins are treated with a nonionic detergent so that the proteins unfold but retain their native ...
... amount of negative and positive charges. This is the isoelectric point of the protein, at which it has no net charge. • For isoelectric focusing, the proteins are treated with a nonionic detergent so that the proteins unfold but retain their native ...
Molecular Inheritance
... 12. Correct The two sugar-phosphate strands of a DNA molecule are joined to each other through _____. (p. 292) Your answer: hydrogen bonds between nucleotide bases Correct. The two sugar-phosphate strands of a DNA molecule are held together by hydrogen bonding between the nitrogenous bases, which ar ...
... 12. Correct The two sugar-phosphate strands of a DNA molecule are joined to each other through _____. (p. 292) Your answer: hydrogen bonds between nucleotide bases Correct. The two sugar-phosphate strands of a DNA molecule are held together by hydrogen bonding between the nitrogenous bases, which ar ...
DNA_Technology_part2
... Identification (stage 4) of bacteria containing the plasmid • Only about 0.001% of bacterial cells take up any DNA/Plasmids when the two are mixed together. • Firstly, we must identify the bacteria containing the plasmids – we do this by growing the bacteria on a medium containing an antibiotic. • ...
... Identification (stage 4) of bacteria containing the plasmid • Only about 0.001% of bacterial cells take up any DNA/Plasmids when the two are mixed together. • Firstly, we must identify the bacteria containing the plasmids – we do this by growing the bacteria on a medium containing an antibiotic. • ...
The On’s and Off’s of Gene Expression
... • Expression of the lac genes is regulated. – The genes are expressed only if lactose is in the growth medium. – The genes are not expressed if glucose is ...
... • Expression of the lac genes is regulated. – The genes are expressed only if lactose is in the growth medium. – The genes are not expressed if glucose is ...
Prof. Emmanuelle Charpentier (France) Dr. Jennifer A. Doudna (USA)
... Already in June of the year following their first meeting, the joint research group used the DNA of streptococcus pyogenes provided by Prof. Charpentier to elucidate the detailed mechanism of how the two RNA and the bacteria’s Cas protein (Cas9) cut foreign DNA. Along with the discovery, they also d ...
... Already in June of the year following their first meeting, the joint research group used the DNA of streptococcus pyogenes provided by Prof. Charpentier to elucidate the detailed mechanism of how the two RNA and the bacteria’s Cas protein (Cas9) cut foreign DNA. Along with the discovery, they also d ...
Plasmids
... In different plasmids the replications regions are linked to different ancillary genes, encoding traits that ordinarily are not essential to the bacterial host but which can, in some environments, provide a biological advantage to bacteria carrying the plasmid. Antibiotic resistance is one of these ...
... In different plasmids the replications regions are linked to different ancillary genes, encoding traits that ordinarily are not essential to the bacterial host but which can, in some environments, provide a biological advantage to bacteria carrying the plasmid. Antibiotic resistance is one of these ...
Lecture 19 Spring 2011
... Transitions—purine for purine and pyrimidine for pyrimidine substitutions, Transversions—purine for pyrimidine and pyrimidine for purine substitutions, and Frameshift mutations—additions or deletions of one or two nucleotide pairs, which alter the reading frame of the gene distal to the site of the ...
... Transitions—purine for purine and pyrimidine for pyrimidine substitutions, Transversions—purine for pyrimidine and pyrimidine for purine substitutions, and Frameshift mutations—additions or deletions of one or two nucleotide pairs, which alter the reading frame of the gene distal to the site of the ...
Document
... Southern blotting is a laboratory technique used to detect a specific DNA sequence in a blood or tissue sample. A restriction enzyme is used to cut a sample of DNA into fragments that are separated using gel electrophoresis. The DNA fragments are transferred out of the gel to the surface of a membra ...
... Southern blotting is a laboratory technique used to detect a specific DNA sequence in a blood or tissue sample. A restriction enzyme is used to cut a sample of DNA into fragments that are separated using gel electrophoresis. The DNA fragments are transferred out of the gel to the surface of a membra ...
Biol 1020: DNA
... DNA replication requires the coordinated activity of many enzymes and other proteins ...
... DNA replication requires the coordinated activity of many enzymes and other proteins ...
Unit #3 Map (2016) Unit_#3_Map_2016
... 7. Double helix: shape of a DNA molecule formed when two twisted DNA strands are coiled into a springlike structure and held together by hydrogen bonds between the bases 8. Gene: sequence of DNA that codes for a protein and thus determines a trait 9. Genotype: genetic makeup of an organism; an organ ...
... 7. Double helix: shape of a DNA molecule formed when two twisted DNA strands are coiled into a springlike structure and held together by hydrogen bonds between the bases 8. Gene: sequence of DNA that codes for a protein and thus determines a trait 9. Genotype: genetic makeup of an organism; an organ ...
Quiz 22
... 2. (a) A large number of DNA copies can be synthesized from a small amount of DNA sample. (1) A large number of DNA copies makes further investigation e.g. DNA fingerprinting possible . (1) (b) (i) Heating breaks the hydrogen bonds between DNA strands / to form two separate strands. (1) (ii) Primers ...
... 2. (a) A large number of DNA copies can be synthesized from a small amount of DNA sample. (1) A large number of DNA copies makes further investigation e.g. DNA fingerprinting possible . (1) (b) (i) Heating breaks the hydrogen bonds between DNA strands / to form two separate strands. (1) (ii) Primers ...
Replicons
... 2 Elongation - When DNA is polymerized 3 Termination - When the new strands of DNA are completed and some finishing touches may be put on the DNA Both elongation and termination may involve proof reading of the DNA ensuring that mutations are not incorporated into newly formed DNA strands ©2001 Timo ...
... 2 Elongation - When DNA is polymerized 3 Termination - When the new strands of DNA are completed and some finishing touches may be put on the DNA Both elongation and termination may involve proof reading of the DNA ensuring that mutations are not incorporated into newly formed DNA strands ©2001 Timo ...
Selective propagation of the clones
... of either strand in vitro with phage RNA polymerases. Infection of transformed bacteria (containing the phagemid) with a helper virus (e.g. derived from M13) will cause the M13 origin to be activated, and progeny viruses carrying single-stranded copies of the phagemid can be obtained. ...
... of either strand in vitro with phage RNA polymerases. Infection of transformed bacteria (containing the phagemid) with a helper virus (e.g. derived from M13) will cause the M13 origin to be activated, and progeny viruses carrying single-stranded copies of the phagemid can be obtained. ...
Slides
... §DNA polymerase II, IV, V - DNA repair enzymes known as translesion repair enzymes §Part of the SOS response - prevent cell death ...
... §DNA polymerase II, IV, V - DNA repair enzymes known as translesion repair enzymes §Part of the SOS response - prevent cell death ...
Crystal structure of actinomycin D bound to the CTG triplet repeat
... Interestingly, when the (CTG)n triplet sequence adopts a hairpin arm (as part of a cruciform) or duplex form between antiparallel CTGs, it contains many GpC binding sites for ActD alternating with T:T mispairs. Previously it has been demonstrated that the binding af®nity to a GpC site is also in¯uen ...
... Interestingly, when the (CTG)n triplet sequence adopts a hairpin arm (as part of a cruciform) or duplex form between antiparallel CTGs, it contains many GpC binding sites for ActD alternating with T:T mispairs. Previously it has been demonstrated that the binding af®nity to a GpC site is also in¯uen ...
Repeated DNA sequences - lecture 1
... First example: ribosomal RNA genes (tandemly repeated, coding). There are 3 genes for rRNA, 18s, 28s and 5.8s. The gene products are part of the ribosome. The 3 genes are organised as a tandemly repeated unit, as shown in the diagram. In humans there are 5 blocks of rRNA repeats, on the short arms o ...
... First example: ribosomal RNA genes (tandemly repeated, coding). There are 3 genes for rRNA, 18s, 28s and 5.8s. The gene products are part of the ribosome. The 3 genes are organised as a tandemly repeated unit, as shown in the diagram. In humans there are 5 blocks of rRNA repeats, on the short arms o ...
DNA Transcription and Translation
... Substitutions also can lead to genetic disorders. Ex. Sickle Cell Anemia (caused by a substitution mutation) Can change both the folding and stability of the protein ...
... Substitutions also can lead to genetic disorders. Ex. Sickle Cell Anemia (caused by a substitution mutation) Can change both the folding and stability of the protein ...
IUSTI Australia MAMEF poster
... In the present study, detection of CT DNA is mediated by a two-step process. First, CT cells are rapidly lysed and the DNA fragmented using lysing chambers composed of gold or aluminum triangles deposited on glass slides and heated using conventional microwave irradiation (Figure 2). After a centrif ...
... In the present study, detection of CT DNA is mediated by a two-step process. First, CT cells are rapidly lysed and the DNA fragmented using lysing chambers composed of gold or aluminum triangles deposited on glass slides and heated using conventional microwave irradiation (Figure 2). After a centrif ...
Course: Biology I Honors Course Code: 2000320 Quarter 2
... combine. Can also be done as a “baby making” project if teachers choose to have students acquire their own genotypes. Note, this is more time consuming and can be done post EOC. -Items referring to general dominant and recessive traits may address but will not assess the P and F1 generations. -Items ...
... combine. Can also be done as a “baby making” project if teachers choose to have students acquire their own genotypes. Note, this is more time consuming and can be done post EOC. -Items referring to general dominant and recessive traits may address but will not assess the P and F1 generations. -Items ...
Chapter 20: Biotechnology - Biology E
... Getting a cloned eukaryotic gene to function in bacterial host cells can be difficult because certain aspects of gene expression are different in eukaryotes and bacteria. To overcome differences in promoters and other DNA control sequences, scientists usually employ an expression vector, a cloning v ...
... Getting a cloned eukaryotic gene to function in bacterial host cells can be difficult because certain aspects of gene expression are different in eukaryotes and bacteria. To overcome differences in promoters and other DNA control sequences, scientists usually employ an expression vector, a cloning v ...
Unit-IV GENETIC ENGINEERING
... 1. DNA is obtained from a source, which may be as small as one cell. 2. Polymerase Chain Reaction PCR is used to make copies of portions of DNA that contains VNTRs. 3. Restriction enzymes are used to cut the VNTRs DNA into pieces so that VNTRs can be detected. 4. The pieces are separated by Electrop ...
... 1. DNA is obtained from a source, which may be as small as one cell. 2. Polymerase Chain Reaction PCR is used to make copies of portions of DNA that contains VNTRs. 3. Restriction enzymes are used to cut the VNTRs DNA into pieces so that VNTRs can be detected. 4. The pieces are separated by Electrop ...
Replisome
The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.