Transformations, Cloning
... Circle and number your colonies. This will make it easier to know which colonies you have selected and will help you if you need to look at the plate again. ...
... Circle and number your colonies. This will make it easier to know which colonies you have selected and will help you if you need to look at the plate again. ...
DNA_extraction
... one tiny corner of the DNA sample to represent that amount. The idea of this exercise is to get the students to think about the relative size of DNA. They need a microscope to see a cell, and one cell’s worth of genetic material makes up less than 1% of that volume, yet for a forensic scientist thes ...
... one tiny corner of the DNA sample to represent that amount. The idea of this exercise is to get the students to think about the relative size of DNA. They need a microscope to see a cell, and one cell’s worth of genetic material makes up less than 1% of that volume, yet for a forensic scientist thes ...
DNA Scientists Formative Assessment
... 3. Hypothesized, in 1952, that protein was the transforming agent in Griffith’s experiment. Used Sulfur and Phosphorus “markers” and discovered that DNA was injected into bacteria. Ultimately concluding that DNA was the transforming agent, not the protein coat. This confirmed what Avery discovered e ...
... 3. Hypothesized, in 1952, that protein was the transforming agent in Griffith’s experiment. Used Sulfur and Phosphorus “markers” and discovered that DNA was injected into bacteria. Ultimately concluding that DNA was the transforming agent, not the protein coat. This confirmed what Avery discovered e ...
78 DNA Cheek Cell lab - Renton School District
... physical traits and regulates body functions. Even though we have had over 50 years of research on DNA, much remains to be learned, and every answer seems to create more questions. The length of DNA per cell is about 100,000x as long as the cell itself. However, it takes up only about 10% of the cel ...
... physical traits and regulates body functions. Even though we have had over 50 years of research on DNA, much remains to be learned, and every answer seems to create more questions. The length of DNA per cell is about 100,000x as long as the cell itself. However, it takes up only about 10% of the cel ...
DNA Sequencing and Gene Analysis
... • Also uses chain terminator nucleotides: dideoxy nucleotides (ddNTPs), which lack the -OH group on the 3' carbon of the deoxyribose. When DNA polymerase inserts one of these ddNTPs into the growing DNA chain, the chain terminates, as nothing can be added to its 3' end. ...
... • Also uses chain terminator nucleotides: dideoxy nucleotides (ddNTPs), which lack the -OH group on the 3' carbon of the deoxyribose. When DNA polymerase inserts one of these ddNTPs into the growing DNA chain, the chain terminates, as nothing can be added to its 3' end. ...
The Structure and Organization of Genetic
... into proteins, composed of linear chains of amino acids (polypeptides). Since 20 different kinds of amino acids are used in this translation, there is a minimum, as well as actual, sequence of three nucleotides necessary to code for any one of these amino acids and for protein termination signals. T ...
... into proteins, composed of linear chains of amino acids (polypeptides). Since 20 different kinds of amino acids are used in this translation, there is a minimum, as well as actual, sequence of three nucleotides necessary to code for any one of these amino acids and for protein termination signals. T ...
DNA: I`m All Split Up
... of DNA.” For the second time, there will be a pairing of nucleotides on the screen. Ask, “How is the DNA code transcribed?” (When a special enzyme, RNA polymerase, encounters a coded start signal on the DNA, the doubled stranded DNA molecule is broken apart and the polymerase begins pairing RNA nuc ...
... of DNA.” For the second time, there will be a pairing of nucleotides on the screen. Ask, “How is the DNA code transcribed?” (When a special enzyme, RNA polymerase, encounters a coded start signal on the DNA, the doubled stranded DNA molecule is broken apart and the polymerase begins pairing RNA nuc ...
dna replication
... DNA replication is the process of duplicating the DNA sequence in the parent strand to produce an exact replica (daughter strand). Replication is semi-conservative: each one of the two parental strands serves as a template for the new strand synthesis; therefore, duplicated double helices contain on ...
... DNA replication is the process of duplicating the DNA sequence in the parent strand to produce an exact replica (daughter strand). Replication is semi-conservative: each one of the two parental strands serves as a template for the new strand synthesis; therefore, duplicated double helices contain on ...
DNA - cloudfront.net
... The first phase of the process involves reading off the __________________ that will be needed to make the protein. The yellow molecule is made up of a close cousin of DNA called ______________. When the RNA copy is complete, it snakes away from the _______________ and moves into the outer part of t ...
... The first phase of the process involves reading off the __________________ that will be needed to make the protein. The yellow molecule is made up of a close cousin of DNA called ______________. When the RNA copy is complete, it snakes away from the _______________ and moves into the outer part of t ...
Molecular Biology
... DNA replication is the process of duplicating the DNA sequence in the parent strand to produce an exact replica (daughter strand). Replication is semi-conservative: each one of the two parental strands serves as a template for the new strand synthesis; therefore, duplicated double helices contain on ...
... DNA replication is the process of duplicating the DNA sequence in the parent strand to produce an exact replica (daughter strand). Replication is semi-conservative: each one of the two parental strands serves as a template for the new strand synthesis; therefore, duplicated double helices contain on ...
molecular_general_theory_complete
... DNA replication is the process of duplicating the DNA sequence in the parent strand to produce an exact replica (daughter strand). Replication is semi-conservative: each one of the two parental strands serves as a template for the new strand synthesis; therefore, duplicated double helices contain on ...
... DNA replication is the process of duplicating the DNA sequence in the parent strand to produce an exact replica (daughter strand). Replication is semi-conservative: each one of the two parental strands serves as a template for the new strand synthesis; therefore, duplicated double helices contain on ...
cloning
... (1) Since processed mRNA have poly A tails they will hydrogen bond to the column matrix (2) DNA, rRNA, tRNA, and proteins will not bind to the matrix and can be rinsed through (3) The mRNA can be eluted from the column with salt of changes in pH 2. Make cDNA copy of mRNA a) Reverse transcriptase (na ...
... (1) Since processed mRNA have poly A tails they will hydrogen bond to the column matrix (2) DNA, rRNA, tRNA, and proteins will not bind to the matrix and can be rinsed through (3) The mRNA can be eluted from the column with salt of changes in pH 2. Make cDNA copy of mRNA a) Reverse transcriptase (na ...
Slide 1
... They are worried that they might be carriers for the disease. Their doctor suggests an amniocentesis to detect if their unborn child has CF or is a carrier. They feel that an amniocentesis is an invasive and risky procedure and decide that they first want to be tested themselves to see if they are c ...
... They are worried that they might be carriers for the disease. Their doctor suggests an amniocentesis to detect if their unborn child has CF or is a carrier. They feel that an amniocentesis is an invasive and risky procedure and decide that they first want to be tested themselves to see if they are c ...
Nerve activates contraction
... activators (like steroid hormones), bound to enhancers to contact the complex at the promoter. ...
... activators (like steroid hormones), bound to enhancers to contact the complex at the promoter. ...
Chapter 12: Genetic Engineering
... What will be the consequences if we develop the ability to “clone” ourselves by making identical genetic copies of our own cells? ...
... What will be the consequences if we develop the ability to “clone” ourselves by making identical genetic copies of our own cells? ...
Work Day 2
... DNA gyrase (removes supercoils) Topoisomerase IV (separates daughter strands) Ciprofloxacin binds to the DNA/enzyme complex forming a physical barrier that prevents movement of the replication fork, RNA polymerase, and DNA helicase ...
... DNA gyrase (removes supercoils) Topoisomerase IV (separates daughter strands) Ciprofloxacin binds to the DNA/enzyme complex forming a physical barrier that prevents movement of the replication fork, RNA polymerase, and DNA helicase ...
Mutations (power point)
... • Mutations are changes in the genetic material of a cell (or virus). • These include large-scale mutations in which long segments of DNA are affected (for example, translocations, duplications, and inversions). • A chemical change in just one base pair of a gene causes a point mutation. • If these ...
... • Mutations are changes in the genetic material of a cell (or virus). • These include large-scale mutations in which long segments of DNA are affected (for example, translocations, duplications, and inversions). • A chemical change in just one base pair of a gene causes a point mutation. • If these ...
BACTERIA TRANSFORMATION LAB (ACTIVITY)
... restriction enzymes. The DNA fragments are spliced together with an enzyme called ligase. Finally the engineered plasmid is taken up by a bacterial cell for replication and expression of the inserted gene. Enzymes play an important role in the formation of recombinant DNA. To excise the desired gene ...
... restriction enzymes. The DNA fragments are spliced together with an enzyme called ligase. Finally the engineered plasmid is taken up by a bacterial cell for replication and expression of the inserted gene. Enzymes play an important role in the formation of recombinant DNA. To excise the desired gene ...
Protein Synthesis: Transcription and Translation
... RNA polymerase recognizes a specific base sequence in the DNA called a promoter and binds to it. The promoter identifies the start of a gene, which strand is to be copied, and the direction that it is to be copied. Complementary bases are assembled (U instead of T). A termination code in the DNA ind ...
... RNA polymerase recognizes a specific base sequence in the DNA called a promoter and binds to it. The promoter identifies the start of a gene, which strand is to be copied, and the direction that it is to be copied. Complementary bases are assembled (U instead of T). A termination code in the DNA ind ...
Chap 11 – Regulation of Eukaryotic Gene Expression
... most genes seems to be off. A typical plant or animal cell needs to turn on and transcribe only a small percentage of its genes. ...
... most genes seems to be off. A typical plant or animal cell needs to turn on and transcribe only a small percentage of its genes. ...
March 13
... introns are self-splicing (type II): no spliceosomes or other enzymes! 2) mRNA editing:many cp mRNAs differ from the gene encoding them •an ACG is modified post-transcriptionally to a functional AUG start codon in several tobacco mRNAs; many other post-transcriptional changes have also been identifi ...
... introns are self-splicing (type II): no spliceosomes or other enzymes! 2) mRNA editing:many cp mRNAs differ from the gene encoding them •an ACG is modified post-transcriptionally to a functional AUG start codon in several tobacco mRNAs; many other post-transcriptional changes have also been identifi ...
Replisome
The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.