Poster
... • With drug bound to topoisomerase, it is able to interact with the DNA of the replicating cell • To achieve contact with DNA, the antibiotic forms a ParC55 closed homodimer with ParE30 (TOPRIM, metal-binding domain) monomers on each side1 • The α1 helix forms a long chain-like Fig. 4 Ciprofloxacin ...
... • With drug bound to topoisomerase, it is able to interact with the DNA of the replicating cell • To achieve contact with DNA, the antibiotic forms a ParC55 closed homodimer with ParE30 (TOPRIM, metal-binding domain) monomers on each side1 • The α1 helix forms a long chain-like Fig. 4 Ciprofloxacin ...
Exam 2 Key v3 Bio200 Win16
... can be made after this instant? Choose the single best answer. ______ An infinite number ___X_ A very large number ______ A small number (less than 10) ______ None ______ A negative number Explain your answer in 1-2 sentences, max. The already-existing mRNA can be repeatedly used to make new protein ...
... can be made after this instant? Choose the single best answer. ______ An infinite number ___X_ A very large number ______ A small number (less than 10) ______ None ______ A negative number Explain your answer in 1-2 sentences, max. The already-existing mRNA can be repeatedly used to make new protein ...
Slide 1
... * It is assumed genes are only encoded meaningfully on the 3' to 5' stand. * Therefore although the 5' to 3' strand will be eaten away, the loss will * consist of complementary base sequences which do not meaningfully encode * genes. Therefore, all we are interested in is the 3' to 5' stand being ea ...
... * It is assumed genes are only encoded meaningfully on the 3' to 5' stand. * Therefore although the 5' to 3' strand will be eaten away, the loss will * consist of complementary base sequences which do not meaningfully encode * genes. Therefore, all we are interested in is the 3' to 5' stand being ea ...
DNA extraction from spider webs | SpringerLink
... This work demonstrates that large fragments of COI (710 bp) can be amplified from a range of spider webs, joining Xu et al. (2015) and Sint et al. (2015) in the recent push toward advancing Araneae conservation genetics. However, caution should be used when using universal primers for species survey ...
... This work demonstrates that large fragments of COI (710 bp) can be amplified from a range of spider webs, joining Xu et al. (2015) and Sint et al. (2015) in the recent push toward advancing Araneae conservation genetics. However, caution should be used when using universal primers for species survey ...
Presentation
... another used a virus with radioactive phosphorus. They infected two batches of bacteria and gave the virus time to attach to the bacteria and inject their hereditary material. The protein coating detached from the original bacteria, but not from the new. The DNA stayed the same in both the new and t ...
... another used a virus with radioactive phosphorus. They infected two batches of bacteria and gave the virus time to attach to the bacteria and inject their hereditary material. The protein coating detached from the original bacteria, but not from the new. The DNA stayed the same in both the new and t ...
DNA Extraction Lab
... 19. You just acted as DNA polymerase above in #18. Describe the function of DNA polymerase: 20. Are there any differences between the two strands? _____________ 21. Are these new double-stranded pieces of DNA the same as or different than the original piece of plant DNA (shown on page 3)?___________ ...
... 19. You just acted as DNA polymerase above in #18. Describe the function of DNA polymerase: 20. Are there any differences between the two strands? _____________ 21. Are these new double-stranded pieces of DNA the same as or different than the original piece of plant DNA (shown on page 3)?___________ ...
Replication of DNA
... on the template strand, then a nucleotide with thymine is added to the newly forming strand. 4. New nucleotides are added in this way until the entire molecule has been copied. 5. Question: If the template strand has the bases ACTGCA, what new complementary strand would be produced? ...
... on the template strand, then a nucleotide with thymine is added to the newly forming strand. 4. New nucleotides are added in this way until the entire molecule has been copied. 5. Question: If the template strand has the bases ACTGCA, what new complementary strand would be produced? ...
DNA Restriction and Gel Electrophoresis This laboratory
... is mainly determined by DNA length. We suspend the DNA (or protein or RNA) in a gel, and then expose the gel to an electric field. The molecule will migrate through the gel when the electric field is applied. We can use different types of gels that have different pore sizes. In this way, we can cho ...
... is mainly determined by DNA length. We suspend the DNA (or protein or RNA) in a gel, and then expose the gel to an electric field. The molecule will migrate through the gel when the electric field is applied. We can use different types of gels that have different pore sizes. In this way, we can cho ...
Chapter 10 Notes
... a) Grew phage in different radioactive elements b) radioactive Sufur to label protein (S only in the proteins), radioactive phosphorous to label DNA (phosphorus only found in the DNA). c) use phage to infect bacteria (E. coli) d) blend to dislodge any phage material stuck to outside of cell e) centr ...
... a) Grew phage in different radioactive elements b) radioactive Sufur to label protein (S only in the proteins), radioactive phosphorous to label DNA (phosphorus only found in the DNA). c) use phage to infect bacteria (E. coli) d) blend to dislodge any phage material stuck to outside of cell e) centr ...
DegenerateInsert
... 12. Have a scalpel blade and a piece of used Xray film ready to use as blades for excision. Also, have a small spatula for manipulating the excised band and clean weigh boat on hand to receive the excised band. When these materials are ready, put on gloves and illuminate the stained gel on a long-wa ...
... 12. Have a scalpel blade and a piece of used Xray film ready to use as blades for excision. Also, have a small spatula for manipulating the excised band and clean weigh boat on hand to receive the excised band. When these materials are ready, put on gloves and illuminate the stained gel on a long-wa ...
9.3 DNA Fingerprinting
... – The probability that two people share identical numbers of repeats in several locations is ...
... – The probability that two people share identical numbers of repeats in several locations is ...
L26_ABPG2014
... ectopic site in double-stranded DNA. Inefficient nicking of the antisense strand forms the primer for full-length cDNA synthesis by the RT with completion of intron insertion by DNA repair. The mechanism on the right begins with reverse splicing into the ectopic site at a replication fork. cDNA synt ...
... ectopic site in double-stranded DNA. Inefficient nicking of the antisense strand forms the primer for full-length cDNA synthesis by the RT with completion of intron insertion by DNA repair. The mechanism on the right begins with reverse splicing into the ectopic site at a replication fork. cDNA synt ...
Molecular Biology I
... The structure of DNA (two complementary strands of DNA) explains: 1)Process of DNA Replication Each strand serves as a template for copying – satisfies model of heredity 2) Occurrence of Mutations Errors in DNA replication (insertion of a noncomplementary base during copying of the DNA) would lead ...
... The structure of DNA (two complementary strands of DNA) explains: 1)Process of DNA Replication Each strand serves as a template for copying – satisfies model of heredity 2) Occurrence of Mutations Errors in DNA replication (insertion of a noncomplementary base during copying of the DNA) would lead ...
Mammoth Reconstruction
... repeats is preserved by the computer. So, the clone-by-clone method is better suited and more accurate (Resch, 2008). Despite the use of both methods, it is important to note that they both use BAC, or bacteria artificial chromosome. Both methods produce multiple small DNA segments. These segments a ...
... repeats is preserved by the computer. So, the clone-by-clone method is better suited and more accurate (Resch, 2008). Despite the use of both methods, it is important to note that they both use BAC, or bacteria artificial chromosome. Both methods produce multiple small DNA segments. These segments a ...
Polymerase Chain Reaction (PCR) + Electrophoresis with agarose
... and cooling of the reaction for DNA melting and enzymatic replication of the DNA. Primers (short DNA fragments, 10-20bp) containing sequences complementary to the target region along with a DNA polymerase (after which the method is named) are key components to enable selective and repeated amplifica ...
... and cooling of the reaction for DNA melting and enzymatic replication of the DNA. Primers (short DNA fragments, 10-20bp) containing sequences complementary to the target region along with a DNA polymerase (after which the method is named) are key components to enable selective and repeated amplifica ...
Transcription to Translation Scavenger Hunt
... Students will have to remember what the start codon is, and also the stop codon. When they are complete they can come to you and show you their mRNA and their protein. Make sure they have both correct, give hints to students who need more help. First group with the correct answer gets candy (optiona ...
... Students will have to remember what the start codon is, and also the stop codon. When they are complete they can come to you and show you their mRNA and their protein. Make sure they have both correct, give hints to students who need more help. First group with the correct answer gets candy (optiona ...
Replisome
The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.