Nucleic Acid Biotechnology Techniques
... growing bacterial colonies contain the plasmid of interest ...
... growing bacterial colonies contain the plasmid of interest ...
Name:
... Protein Synthesis #1: Transcription and Translation Practice Transcription directions: Transcribe the following DNA sequence into messenger RNA (mRNA.) It’s easiest to break the DNA sequence into triplets, and then find the mRNA codons from that point: i.e. AGA TTC CCC DNA triplets transcription UCU ...
... Protein Synthesis #1: Transcription and Translation Practice Transcription directions: Transcribe the following DNA sequence into messenger RNA (mRNA.) It’s easiest to break the DNA sequence into triplets, and then find the mRNA codons from that point: i.e. AGA TTC CCC DNA triplets transcription UCU ...
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... •A single stranded RNA will have OH groups at the 2’ positions •Note the directionality of DNA or RNA ...
... •A single stranded RNA will have OH groups at the 2’ positions •Note the directionality of DNA or RNA ...
Introduction to Organic Chemistry and Biochemistry
... Reactions that take place in cells often involve enzymes which speed up chemical reactions ( often by factors of a million or more) by lowering the energy barrier that must be overcome to go from reactants to products. For example many cells contain an enzyme called catalase (or hydrogen peroxidase) ...
... Reactions that take place in cells often involve enzymes which speed up chemical reactions ( often by factors of a million or more) by lowering the energy barrier that must be overcome to go from reactants to products. For example many cells contain an enzyme called catalase (or hydrogen peroxidase) ...
Paper Plasmids Lab
... Some of the most important techniques used in biotechnology today involve making recombinant DNA molecules. A recombinant object has been reassembled from parts taken from more than one source. Your genome is recombinant in that part of ,it came from your mother and part came from your father. Recom ...
... Some of the most important techniques used in biotechnology today involve making recombinant DNA molecules. A recombinant object has been reassembled from parts taken from more than one source. Your genome is recombinant in that part of ,it came from your mother and part came from your father. Recom ...
Recombinant Paper Plasmids:
... You have now prepared a pAMP plasmid and a pKAN plasmid. In this pare of the activity, you will use them as starting materials to make a recombinant plasmid. You will cut pAMP and pKAN with two specific enzymes, BamHI and HindIII. You will ligate together fragments that come from each plasmid, creat ...
... You have now prepared a pAMP plasmid and a pKAN plasmid. In this pare of the activity, you will use them as starting materials to make a recombinant plasmid. You will cut pAMP and pKAN with two specific enzymes, BamHI and HindIII. You will ligate together fragments that come from each plasmid, creat ...
DNA Packing
... 1. Sample is heated to separate DNA strands 2. Sample is cooled and primer binds to specific target sequence 3. Target sequence is copied with DNA polymerase ...
... 1. Sample is heated to separate DNA strands 2. Sample is cooled and primer binds to specific target sequence 3. Target sequence is copied with DNA polymerase ...
The Genetic Material
... • The bacterial chromosome is found in a region of the cell called the nucleoid (not enclosed in membrane) • Bacterial chromosomal DNA is usually a circular molecule that is a few million nucleotides in length – Escherichia coli ~ 4.6 million base pairs – Haemophilus influenzae ~ 1.8 million bas ...
... • The bacterial chromosome is found in a region of the cell called the nucleoid (not enclosed in membrane) • Bacterial chromosomal DNA is usually a circular molecule that is a few million nucleotides in length – Escherichia coli ~ 4.6 million base pairs – Haemophilus influenzae ~ 1.8 million bas ...
Chapter 14 Transposons, Plasmids, and Bacteriophage
... occurs, in case after c+ gene has been transferred but before d gene has entered recipient cell. ...
... occurs, in case after c+ gene has been transferred but before d gene has entered recipient cell. ...
ara Operon
... • Study of biological processes (example: synthesis of proteins) • Localization and regulation of gene expression • Cell movement • Cell fate during development ...
... • Study of biological processes (example: synthesis of proteins) • Localization and regulation of gene expression • Cell movement • Cell fate during development ...
History of DNA
... • 1950 Erwin Chargaff – Not all DNA has the same base composition • Varies from one species to another ...
... • 1950 Erwin Chargaff – Not all DNA has the same base composition • Varies from one species to another ...
Factors Affecting the Absorption Properties of Chromophore
... effects on Λmax and e. Spectra of DNA as a double stranded DNA differs from a single stranded DNA or after hydrolysis to free nucleuotides. ...
... effects on Λmax and e. Spectra of DNA as a double stranded DNA differs from a single stranded DNA or after hydrolysis to free nucleuotides. ...
Linkage
... Terminology in microbial genetics • Prototroph: “original” and “feed”, a wild type strain, one able to synthesize all needed compounds from a simple carbon source such as glucose. • Auxotroph: a mutant that has lost the ability to make some necessary organic compound; it must be added to the cultur ...
... Terminology in microbial genetics • Prototroph: “original” and “feed”, a wild type strain, one able to synthesize all needed compounds from a simple carbon source such as glucose. • Auxotroph: a mutant that has lost the ability to make some necessary organic compound; it must be added to the cultur ...
Katie Snape (Genetics Update)
... • Reads = the strands of DNA which are aligned with the reference sequence • Depth of coverage = number of reads covering a particular region of the exome – The deeper the coverage, the more accurate the ...
... • Reads = the strands of DNA which are aligned with the reference sequence • Depth of coverage = number of reads covering a particular region of the exome – The deeper the coverage, the more accurate the ...
Document
... Change in one or a few base pairs in a gene. These are very small changes when compared with the large-scale mutations. Point mutations can be divided into two general categories: ...
... Change in one or a few base pairs in a gene. These are very small changes when compared with the large-scale mutations. Point mutations can be divided into two general categories: ...
HO DNA Necklace Lab Report
... minimum of 10 minutes. During this time, DNA will continue to precipitate out of solution and extend like a ribbon through the entire ethanol layer. DNA yields will naturally vary within the class and not all DNA samples will extend through the entire ethanol layer. 9. Watch closely as wispy strands ...
... minimum of 10 minutes. During this time, DNA will continue to precipitate out of solution and extend like a ribbon through the entire ethanol layer. DNA yields will naturally vary within the class and not all DNA samples will extend through the entire ethanol layer. 9. Watch closely as wispy strands ...
Troubling and Terrific Technology
... restriction enzyme, the enzyme cuts it in the same place every time. This leaves the same fragments of DNA every time it is exposed to the restriction enzyme Sticky ends - restriction fragments can be staggered, leaving short stretches of single stranded DNA (sticky ends). These single stranded area ...
... restriction enzyme, the enzyme cuts it in the same place every time. This leaves the same fragments of DNA every time it is exposed to the restriction enzyme Sticky ends - restriction fragments can be staggered, leaving short stretches of single stranded DNA (sticky ends). These single stranded area ...
Replisome
The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.