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DNA, RNA, and the Flow of Genetic Information
DNA, RNA, and the Flow of Genetic Information

... DNA are derivatives of purine—adenine (A) and guanine (G)—and two of pyrimidine—cytosine (C) and thymine (T), as shown in Figure 4.4. Ribonucleic acid (RNA), like DNA, is a long unbranched polymer consisting of nucleotides joined by 39-to-59 phosphodiester linkages (see Figure 4.3). The covalent str ...
The Genetics of Bacteria and Their Viruses
The Genetics of Bacteria and Their Viruses

... jump from one position to another or from one DNA molecule to another • Bacteria contain a wide variety of transposable elements (as do all other organisms studied to date) • The smallest and simplest are insertion sequences, or IS elements, which are 1–3 kb in length and encode the transposase prot ...
DNA and Evolution
DNA and Evolution

Poster
Poster

... RNA polymerase (RNAP) is an information-processing molecular machine that copies DNA into RNA. It is a multi-subunit complex found in every living organism. Bacterial RNAP contains six subunits ( ’ 2 ). The ’ subunits form several distinct functional channels that accommodate double stranded DNA and ...
DNA profiling - Our eclass community
DNA profiling - Our eclass community

... of cutting and pasting a foreign piece of DNA into the DNA of a cell.  It brings together genetic material from multiple sources, creating new sequences of DNA.  Enables the genome to be manipulated very precisely ...
16_Lecture_Presentation
16_Lecture_Presentation

... stabilizes single-stranded DNA until it can be used as a template know this ...
CHAPTER 18
CHAPTER 18

... and –10: 5’–TATAAT–3’. Most mutations that alter the consensus sequence would be expected to decrease the rate of transcription. For example, a mutation that changed the – 35 region to 5’–GAGACA–3’ would decrease transcription. The sequence 5’–TATAAT–3’ is recognized by the transcription factor TFII ...
Conceptual Questions C1. Answer: A. G→A, which is a transition. B
Conceptual Questions C1. Answer: A. G→A, which is a transition. B

The Differential Killing of Genes by Inversions in Prokaryotic Genomes
The Differential Killing of Genes by Inversions in Prokaryotic Genomes

... strand is located on the leading strand, it is assumed that “the gene lies on the leading strand.” In prokaryotic genomes, the leading and lagging roles of DNA strands are predetermined by location of the origin of replication and the terminus of replication. Thus, a gene stays at the same position ...
Transcription - SCIS Teachers
Transcription - SCIS Teachers

Introduction to cell biology
Introduction to cell biology

... and maintain a living organism. This hereditary information is passed on from a cell to its daughter cells at cell division. In the 1940s, deoxyribonucleic acid (DNA) was identified as the likely carrier of genetic information [1]. The building blocks of the DNA molecule are called nucleotides. The ...
Recombinant DNA Answer Key
Recombinant DNA Answer Key

... Copying DNA Genetic engineers can transfer a gene from one organism to another to achieve a goal, but first, individual genes must be identified and separated from DNA. The original method (used by Douglas Prasher) involved several steps: ▶ Determine the amino acid sequence in a protein. ▶ Predict t ...
1.2.3.A DNAAnalysisF - Clayton School District
1.2.3.A DNAAnalysisF - Clayton School District

... because of this fact that DNA is so valuable in many criminal investigations. A suspect can be identified using his or her DNA profile. In 1984, a British scientist name Alec Jeffreys developed a technique utilizing the variation in DNA sequences to identify individuals. Restriction endonucleases (c ...
Recombinant DNA Biotech Summary Questions
Recombinant DNA Biotech Summary Questions

What are the methods and approaches used to identify and
What are the methods and approaches used to identify and

... Matt Emmer Jason Chai ...
Supporting information PCR amplification and DGGE analysis The
Supporting information PCR amplification and DGGE analysis The

... determined by comparison with the internal GeneScan™ 500 LIZ® Size Standard. Three ...
figure 9-9
figure 9-9

... twentieth-century biology and promises a vastly changed scientific landscape for the new century.  In international cooperative research efforts, the genomes ...
Problem Set 1 Questions
Problem Set 1 Questions

... 19. You have two different derivatives of fibroblast cultures: one grows as completely flat cells, and the other grows as round, ragged cells. When you mix flat cells with extracts prepared from live round ragged cells, all the flat cells become round and ragged. These cells continue to grow as roun ...
Summary notes for ch1-6
Summary notes for ch1-6

... - Replication of DNA by DNA polymerase and primer. - DNA is unwound and unzipped to form two template strands. - DNA polymerase can only add nucleotides to a pre-existing chain, so to begin to function a primer must be present. - A primer is a short sequence of nucleotides formed at the 3’ end of th ...
Nucleic Acids – Organic/Macromolecule #4
Nucleic Acids – Organic/Macromolecule #4

... cytosine. DNA is made up of two strands of nucleotides, hooked together in a twisted ladder shape called a double helix. The two strands are held together by complementary bases on opposite strands. Thymine has a shape that complements (fits together like a puzzle) adenine. So every nucleotide that ...
Nucleic Acids – Organic/Macromolecule #4
Nucleic Acids – Organic/Macromolecule #4

... cytosine. DNA is made up of two strands of nucleotides, hooked together in a twisted ladder shape called a double helix. The two strands are held together by complementary bases on opposite strands. Thymine has a shape that complements (fits together like a puzzle) adenine. So every nucleotide that ...
Molecular Genetics Close Notes Booklet
Molecular Genetics Close Notes Booklet

... Mutations come in 3 main varieties: Mutations generally result in a protein that does not function as well or does not function at all. In some rare cases, mutations can provide an advantage and be beneficial. These changes may give that organism a competitive advantage. ...
Recombination - CCGB | index
Recombination - CCGB | index

... Recombination • Breaking and rejoining of two parental DNA molecules to produce new DNA molecules • Reciprocal recombination: new DNA molecules carry genetic information from both parental molecules. • Gene conversion: one way transfer of information, resulting in an allele on one parental chromoso ...
Next lectures: Differential Gene expression
Next lectures: Differential Gene expression

... Site 2 Site 1 ...
Chapter 4 part I
Chapter 4 part I

... • Oligonucleotides (C and D) that are complementary to the ends of the product of the first PCR cycle are added. • Overlapping molecules are formed after denaturation and renaturation, and the recessed ends are filled. • Oligonucleotides (E and F) that overlapped the ends of the second PCR cycle pro ...
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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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