Density Gradient Centrifugation
... the actual path taken by bio-molecules is much longer than the length of the gel allowing for better separation. (Think about the resolution obtained on a chromatographic column) As shown before, one of the clever methods to sequence DNA in seventies was to subject single stranded DNA to specific en ...
... the actual path taken by bio-molecules is much longer than the length of the gel allowing for better separation. (Think about the resolution obtained on a chromatographic column) As shown before, one of the clever methods to sequence DNA in seventies was to subject single stranded DNA to specific en ...
Genetics - wongweicong
... Journey into dna We almost see the DNA strand as we look at some nucleosomes. It’s wrapped tightly on those round things, Which are structures called protein histones. The histones, they carry a plus charge; without it the strand’s in distress. For then the DNA would try to float free, And become a ...
... Journey into dna We almost see the DNA strand as we look at some nucleosomes. It’s wrapped tightly on those round things, Which are structures called protein histones. The histones, they carry a plus charge; without it the strand’s in distress. For then the DNA would try to float free, And become a ...
DNA 101 intro
... Sequence DNA Replication • Put the following sentences into the correct order: • 3. Enzymes add free nucleotides to complement bases on both strands of DNA • 1. DNA is unwound with enzymes • 2. DNA is unzipped between complementary bases with enzymes • 4.Two new strands of DNA are made • 5. This pr ...
... Sequence DNA Replication • Put the following sentences into the correct order: • 3. Enzymes add free nucleotides to complement bases on both strands of DNA • 1. DNA is unwound with enzymes • 2. DNA is unzipped between complementary bases with enzymes • 4.Two new strands of DNA are made • 5. This pr ...
Prot-DNAInteractionsWksht
... 6) List five protein-DNA interactions observed in the Protein X complex with DNA (Kinemage 1) that are independent of the specific sequence of the operon. Conformational changes 7) Use the dynamic 3-D information in Kinemage 2 to describe Protein X’s conformational changes for apo vs liganded vs DNA ...
... 6) List five protein-DNA interactions observed in the Protein X complex with DNA (Kinemage 1) that are independent of the specific sequence of the operon. Conformational changes 7) Use the dynamic 3-D information in Kinemage 2 to describe Protein X’s conformational changes for apo vs liganded vs DNA ...
demonstating sequence-specific cleavage by a restriction enzyme
... bacteriophage P22. Using a device called a viscometer, he measured how the DNA from P22 became less viscous over time, while the H. influenzae DNA displayed no change in viscosity. This would be the assay he would use throughout the purification scheme. Smith used a variety of established methods to ...
... bacteriophage P22. Using a device called a viscometer, he measured how the DNA from P22 became less viscous over time, while the H. influenzae DNA displayed no change in viscosity. This would be the assay he would use throughout the purification scheme. Smith used a variety of established methods to ...
CHS H Bio Final Exam Review Sheet
... three types of nucleotides be? What does semi-conservative mean with respect to DNA replication? What enzyme creates the new chains of DNA during replication? List 3 differences between DNA and RNA. What are the three types of RNA discussed in class and what are their functions? What is a codon? An ...
... three types of nucleotides be? What does semi-conservative mean with respect to DNA replication? What enzyme creates the new chains of DNA during replication? List 3 differences between DNA and RNA. What are the three types of RNA discussed in class and what are their functions? What is a codon? An ...
SUNY-ESF Web
... initiating nucleotide on the sense strand of the E. coli tRNA Tyr promoter shown below.. . . 5’CAACGTAACACTTTACAGCGGCGCGTCATTTGATATGATGCGCCCCGCTTCCCGATA3’. 3’GTTGCATTGTGAAATGTCGCCGCGCAGTAAACTATACTACGCGGGGCGAAGGGCTA T-5’. . What are the main differences between the prokaryotic RNAP and eukary ...
... initiating nucleotide on the sense strand of the E. coli tRNA Tyr promoter shown below.. . . 5’CAACGTAACACTTTACAGCGGCGCGTCATTTGATATGATGCGCCCCGCTTCCCGATA3’. 3’GTTGCATTGTGAAATGTCGCCGCGCAGTAAACTATACTACGCGGGGCGAAGGGCTA T-5’. . What are the main differences between the prokaryotic RNAP and eukary ...
Biotechnology Lab
... Plasmid DNA – extrachromosomal DNA (“bonus material”) useful for experimental manipulation; circular, double-stranded ...
... Plasmid DNA – extrachromosomal DNA (“bonus material”) useful for experimental manipulation; circular, double-stranded ...
8.4 Transcription
... Where will the RNA transcript go after it separates from the DNA in step 3? • It is processed and LEAVES the nucleus, heads to the ...
... Where will the RNA transcript go after it separates from the DNA in step 3? • It is processed and LEAVES the nucleus, heads to the ...
Chapter 10
... short tandem repeat (STR)—short segment of DNA in which the same sequence of two to six base pairs is repeated many times; varying numbers of repeats found among individuals ...
... short tandem repeat (STR)—short segment of DNA in which the same sequence of two to six base pairs is repeated many times; varying numbers of repeats found among individuals ...
TRANSPOSABLE ELEMENTS IN BACTERIA Transposable
... IS elements consist of a relatively short (700-1500 bp) DNA segment flanked by a 10-40 bp inverted terminal repeat (ITR) sequence. A complete IS element codes for the protein (transposase) that catalyses the transposition event. Thus, transposition requires that the IS element carry a promoter recog ...
... IS elements consist of a relatively short (700-1500 bp) DNA segment flanked by a 10-40 bp inverted terminal repeat (ITR) sequence. A complete IS element codes for the protein (transposase) that catalyses the transposition event. Thus, transposition requires that the IS element carry a promoter recog ...
Biogenetic Engineering & Manipulating Genes
... – PCR rapidly amplifies a single DNA molecule into many billions of molecules. ...
... – PCR rapidly amplifies a single DNA molecule into many billions of molecules. ...
DNA is Composed of Complementary Strands
... • Degrade DNA in a stepwise manner by removing deoxynucleotides in 5’ 3’ (A) or 3’ 5’ direction (B) • Require a free OH • Most exonucleases are active on both single- and double-stranded DNA • Used for degrading foreign DNA and in proofreading during DNA synthesis ...
... • Degrade DNA in a stepwise manner by removing deoxynucleotides in 5’ 3’ (A) or 3’ 5’ direction (B) • Require a free OH • Most exonucleases are active on both single- and double-stranded DNA • Used for degrading foreign DNA and in proofreading during DNA synthesis ...
Design Genes with Ease Using In-Fusion® Cloning
... of poxvirus DNA polymerase (2–4). When incubated with linear duplex DNAs with identical ends in the presence of Mg2+ and low concentrations of dNTP, the 3'–5' proofreading activity of poxvirus DNA polymerase progressively removes nucleotides from the 3' end. This exposes complementary regions on the ...
... of poxvirus DNA polymerase (2–4). When incubated with linear duplex DNAs with identical ends in the presence of Mg2+ and low concentrations of dNTP, the 3'–5' proofreading activity of poxvirus DNA polymerase progressively removes nucleotides from the 3' end. This exposes complementary regions on the ...
Molecular Genetics Test
... (3.) Tryptophan (trp) (4.) Glutamic acid (glu) (5.) Asparic acid (asp) 62. The "one gene one protein " theory can be explained how genes control in cells by (1.) determining polypeptide chains (2.) interacting with proteins in the chromosomes (3.) assorting independently during meiosis (4.) inhibiti ...
... (3.) Tryptophan (trp) (4.) Glutamic acid (glu) (5.) Asparic acid (asp) 62. The "one gene one protein " theory can be explained how genes control in cells by (1.) determining polypeptide chains (2.) interacting with proteins in the chromosomes (3.) assorting independently during meiosis (4.) inhibiti ...
File - Intervention
... the four nitrogenous bases. The particular order of bases within a gene determines the product of the gene. Each organism is genetically different because of the sequence of their bases. Deoxyribonucleic acid (DNA) is the master copy of an organism’s genetic information. The information in DNA con ...
... the four nitrogenous bases. The particular order of bases within a gene determines the product of the gene. Each organism is genetically different because of the sequence of their bases. Deoxyribonucleic acid (DNA) is the master copy of an organism’s genetic information. The information in DNA con ...
Gene Cloning
... Cloning allows full characterisation of a gene including identification and analysis of regulatory sequences and mechanisms controlling spatial and temporal gene expression (i.e. when and where the gene is expressed) by; DNA sequence analysis Determination of 5' and 3' ends of the mRNA transcript Lo ...
... Cloning allows full characterisation of a gene including identification and analysis of regulatory sequences and mechanisms controlling spatial and temporal gene expression (i.e. when and where the gene is expressed) by; DNA sequence analysis Determination of 5' and 3' ends of the mRNA transcript Lo ...
TheScienceofSuperAmber
... way) is replicated in a continuous piece and is called the “leading strand.” The other half of the template would require building a DNA strand in the 3’ to 5’ direction, which is not possible, so this strand is made by synthesizing little 5’ to 3’ fragments at a time (called Okazaki fragments) and ...
... way) is replicated in a continuous piece and is called the “leading strand.” The other half of the template would require building a DNA strand in the 3’ to 5’ direction, which is not possible, so this strand is made by synthesizing little 5’ to 3’ fragments at a time (called Okazaki fragments) and ...
Transcription and Translation - Microbiology and Molecular Genetics
... build phosphodiesterase bonds ...
... build phosphodiesterase bonds ...
Replisome
The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.