LacZ Reporter Gene Expression in 81 KOMP Heterozygous Mutants
... sections has a similar anatomical distribution and is observed less frequently than that found with whole-mounts. ...
... sections has a similar anatomical distribution and is observed less frequently than that found with whole-mounts. ...
HMG 9_9.book(ddd146.fm)
... additional mutations located also in exon 3 (R168X, R255X, R306C, P322A and 1194insT). We analysed DNA samples from both parents of all individuals with a MECP2 mutation and none of the parents’ samples showed any abnormalities by DGGE or restriction analysis, demonstrating that these are de novo mu ...
... additional mutations located also in exon 3 (R168X, R255X, R306C, P322A and 1194insT). We analysed DNA samples from both parents of all individuals with a MECP2 mutation and none of the parents’ samples showed any abnormalities by DGGE or restriction analysis, demonstrating that these are de novo mu ...
DNA methylation profiling identifies epigenetic dysregulation in
... Type 2 diabetes (T2D) has developed into a major public health concern. While previously considered as a problem primarily for western populations, the disease is rapidly gaining global importance, as today around 285 million people are affected worldwide (IDF, 2009). Lifestyle and behavioural facto ...
... Type 2 diabetes (T2D) has developed into a major public health concern. While previously considered as a problem primarily for western populations, the disease is rapidly gaining global importance, as today around 285 million people are affected worldwide (IDF, 2009). Lifestyle and behavioural facto ...
Nature Genetics: doi:10.1038/ng.3791
... average, each 1kb window has 77 bases filtered out by the UMM, corresponding to 3.7% of all sites. The efficiency of the UMM filter also manifests in the tssQTL set, where the percentage of lead variants being an indel (11%) is lower compared to the tested variant set as a whole (18%). This is expec ...
... average, each 1kb window has 77 bases filtered out by the UMM, corresponding to 3.7% of all sites. The efficiency of the UMM filter also manifests in the tssQTL set, where the percentage of lead variants being an indel (11%) is lower compared to the tested variant set as a whole (18%). This is expec ...
Extended Life-Span and Stress Resistance in Drosophila
... • Most long-lived daf-2 and InR mutants are also dwarfs with low fertility, but some long-lived InR mutants have normal size and fertility, indicating that longevity may be regulated independently of body size and fertility. • daf-2 and InR are structural homologs of a family of vertebrate receptor ...
... • Most long-lived daf-2 and InR mutants are also dwarfs with low fertility, but some long-lived InR mutants have normal size and fertility, indicating that longevity may be regulated independently of body size and fertility. • daf-2 and InR are structural homologs of a family of vertebrate receptor ...
Whole-genome expression analysis of snf swi mutants of
... The factors that determine the dependence of a gene on Snf兾Swi are not understood. Several studies have indicated that Snf兾Swi may be targeted to particular promoters by physical interactions with specific transcriptional activators or repressors (11–18). However, it seems likely that such interacti ...
... The factors that determine the dependence of a gene on Snf兾Swi are not understood. Several studies have indicated that Snf兾Swi may be targeted to particular promoters by physical interactions with specific transcriptional activators or repressors (11–18). However, it seems likely that such interacti ...
Chromatin DNA Methylayion
... Agents that inhibit DNA methyltransferase such as 5-aza-CdR stimulate tumor invasion and metastasis. ...
... Agents that inhibit DNA methyltransferase such as 5-aza-CdR stimulate tumor invasion and metastasis. ...
CHAPTER 7 TUNING THE DRAGON PROMOTER FINDER SYSTEM FOR HUMAN PROMOTER RECOGNITION
... the G+C-rich and G+C-poor groups. This separation of data and subsequent development of models for both of these promoter groups, as well as the sophisticated tuning of the models, has resulted in considerably enhanced system performance. The resulting system combines: multiple hierarchically organi ...
... the G+C-rich and G+C-poor groups. This separation of data and subsequent development of models for both of these promoter groups, as well as the sophisticated tuning of the models, has resulted in considerably enhanced system performance. The resulting system combines: multiple hierarchically organi ...
Driscoll Katee Driscoll Dr. Ely Genetics October 20, 2013 Effects of
... Optimization procedures were applied to the models in order to produce the optimal prediction model, which relied on the method of splitting the data (using some of the data as a training set to predict the HTT CAG repeat lengths for the remaining samples that comprised the other set) (Lee et al. 20 ...
... Optimization procedures were applied to the models in order to produce the optimal prediction model, which relied on the method of splitting the data (using some of the data as a training set to predict the HTT CAG repeat lengths for the remaining samples that comprised the other set) (Lee et al. 20 ...
not a plastid specific promoter but is also capable of
... and the parental 35S promoter construction (pGSFR28O, 10) were transiently expressed in SRI protoplasts. The expression level of PAT directed by pGSCMI100 is only tenfold below that of pGSFR28O (fig. 4b). This is an increase of several orders of magnitude with respect to the original psbA bar gene a ...
... and the parental 35S promoter construction (pGSFR28O, 10) were transiently expressed in SRI protoplasts. The expression level of PAT directed by pGSCMI100 is only tenfold below that of pGSFR28O (fig. 4b). This is an increase of several orders of magnitude with respect to the original psbA bar gene a ...
Characterization of Chicken MMP13 Expression and Genetic Effect
... follicles of sexually immature chicken ovaries, in the theca cell layers of all sized follicles of sexually mature chicken ovaries. Furthermore, we identified a positive element (positions –1863 to –1036) controlling chicken MMP13 transcription, and, in this region, six single nucleotide polymorphism ...
... follicles of sexually immature chicken ovaries, in the theca cell layers of all sized follicles of sexually mature chicken ovaries. Furthermore, we identified a positive element (positions –1863 to –1036) controlling chicken MMP13 transcription, and, in this region, six single nucleotide polymorphism ...
Protein expression pattern in cerebellum of Cav2.1 mutant, tottering
... products were detected in the fractions that did not include cDNA (data not shown). Histological observation of protein expression patterns in the cerebellum of tottering-6j mice We have used cresyl violet staining to check the gross morphology and cytoarchitecture. The gross morphology (data not sh ...
... products were detected in the fractions that did not include cDNA (data not shown). Histological observation of protein expression patterns in the cerebellum of tottering-6j mice We have used cresyl violet staining to check the gross morphology and cytoarchitecture. The gross morphology (data not sh ...
the Liver - Saudi Medical Journal
... Recurrent spontaneous abortion (RSA) is defined as "three or more consecutive pregnancy losses before the 20th week of gestation"(1,2). Several factors have been implicated as causative factors in the etiology of abortion, including genetic and environmental factors. However, in almost 50% of the ca ...
... Recurrent spontaneous abortion (RSA) is defined as "three or more consecutive pregnancy losses before the 20th week of gestation"(1,2). Several factors have been implicated as causative factors in the etiology of abortion, including genetic and environmental factors. However, in almost 50% of the ca ...
Six3, a murine homologue of the sine oculis gene, demarcates the
... brain cDNA library was screened at low stringency using the previously isolated Six1 cDNA as probe. Approximately 106 plaques were screened and the positive clones were further purified. The largest cDNA insert isolated from these clones was of 1.8 kb (see Materials and Methods). Sequence analysis s ...
... brain cDNA library was screened at low stringency using the previously isolated Six1 cDNA as probe. Approximately 106 plaques were screened and the positive clones were further purified. The largest cDNA insert isolated from these clones was of 1.8 kb (see Materials and Methods). Sequence analysis s ...
Construction of transformation vectors and plant
... In plants regenerated from transformed calli the constitutive expression of gus gene drove by three promoters was verified: GUS staining was confirmed in leaves, stems and roots of all the plants. But some differences were observed in the staining pattern depending on the promoter. Using A9 promoter ...
... In plants regenerated from transformed calli the constitutive expression of gus gene drove by three promoters was verified: GUS staining was confirmed in leaves, stems and roots of all the plants. But some differences were observed in the staining pattern depending on the promoter. Using A9 promoter ...
Two enhancer regions in the mouse En-2 locus
... analyzed for lacZ activity as described below. The day on which a vaginal plug was observed was designated day 0.5 of gestation. In one of the transgenic lines, Tg4.35, analysis of DNA extracted from the founder revealed that the transgene had inserted at two different sites within the genome. These ...
... analyzed for lacZ activity as described below. The day on which a vaginal plug was observed was designated day 0.5 of gestation. In one of the transgenic lines, Tg4.35, analysis of DNA extracted from the founder revealed that the transgene had inserted at two different sites within the genome. These ...
Changes in DNA-binding activity of transcription factors in the
... Our earlier studies carried out in silico demonstrated that different transcription factors have their putative binding sites in the 5’-flanking regions of bovine milk protein genes. Now we extended our study to include the experimental analysis of these transcription factors. This study on electrop ...
... Our earlier studies carried out in silico demonstrated that different transcription factors have their putative binding sites in the 5’-flanking regions of bovine milk protein genes. Now we extended our study to include the experimental analysis of these transcription factors. This study on electrop ...
Coffee, B, Zhang, F, Warren, ST and Reines, D: Acetylated histones are associated with the FMR1 gene in normal but not fragile X syndrome cells. Nature Genetics 22:98-101 (1999).
... patients whose FMR1 gene is transcriptionally silent23,24. These results are consistent with a model in which CGG-repeat expansion and methylation of FMR1 result in the recruitment of transcriptional silencing machinery to the gene, followed by loss of transcription. The inability to reactivate tran ...
... patients whose FMR1 gene is transcriptionally silent23,24. These results are consistent with a model in which CGG-repeat expansion and methylation of FMR1 result in the recruitment of transcriptional silencing machinery to the gene, followed by loss of transcription. The inability to reactivate tran ...
MetaXcan: Summary Statistics Based Gene-Level
... Over the last decade, GWAS have been successful in identifying genetic loci that robustly associate with multiple complex traits. However, the mechanistic understanding of these discoveries is still limited, hampering the translation of this knowledge into actionable targets. Studies of enrichment o ...
... Over the last decade, GWAS have been successful in identifying genetic loci that robustly associate with multiple complex traits. However, the mechanistic understanding of these discoveries is still limited, hampering the translation of this knowledge into actionable targets. Studies of enrichment o ...
Regulation of the S100B gene by α1-adrenergic - AJP
... (PKC) signaling pathway. In the present study, we examined whether the same pathway induced the S100B gene, supporting the hypothesis that S100B is a feedback negative regulator of this pathway. We transfected cultured neonatal rat cardiac myocytes with a luciferase reporter gene driven by the maxim ...
... (PKC) signaling pathway. In the present study, we examined whether the same pathway induced the S100B gene, supporting the hypothesis that S100B is a feedback negative regulator of this pathway. We transfected cultured neonatal rat cardiac myocytes with a luciferase reporter gene driven by the maxim ...
psychometric properties of the depression - Site BU
... used to examine the structure of the scale in a clinical sample. Using the entire study sample (N = 437), principal components extraction with varimax rotation was performed (orthogonal rotation was used to assist in the interpretation and description of the resulting factor loadings). Selection of ...
... used to examine the structure of the scale in a clinical sample. Using the entire study sample (N = 437), principal components extraction with varimax rotation was performed (orthogonal rotation was used to assist in the interpretation and description of the resulting factor loadings). Selection of ...
Development of an Ethologically-Relevant Chronic Stress Model of
... development of anhedonia, a core symptom of depression, assessed by the decrease in sucrose intake and preference. The chronic stress paradigm also leads to locomotor changes, anxietylike behavior and impairment in memory and learning. The aim of this project was to study the effects of the applicat ...
... development of anhedonia, a core symptom of depression, assessed by the decrease in sucrose intake and preference. The chronic stress paradigm also leads to locomotor changes, anxietylike behavior and impairment in memory and learning. The aim of this project was to study the effects of the applicat ...
Combined expression patterns of QTL
... For instance, catecholamines up (catsup) is associated with naturally occurring variation in multiple traits such as locomotor behavior and longevity (Carbone et al., 2006), Additionally, we also tested two genes that are either up-regulated (CG10383) and down-regulated (cyp6a13) early in the heat-s ...
... For instance, catecholamines up (catsup) is associated with naturally occurring variation in multiple traits such as locomotor behavior and longevity (Carbone et al., 2006), Additionally, we also tested two genes that are either up-regulated (CG10383) and down-regulated (cyp6a13) early in the heat-s ...